فهرست مطالب mahdi rahpeyma

Angiogenesis is the formation of new blood capillaries, and it is important for physiological processes such as growth and development and pathological conditions such as tumor growth and metastasis. Angiogenesis inhibition is considered very useful in the prevention and treatment of cancer. Matrix metalloproteinases (MMPs) are a family approximately consists of 28 zinc-dependent content endopeptidase which degrade the extracellular matrix (ECM) and play an important role on angiogenesis development and tumor metastasis. Therefore, inhibition of MMPs prevents the angiogenesis. The expression and levels of MMP-2 and MMP-9 is increased in many human tumors, including ovarian, breast and prostate tumors. In this study, cytotoxic effects of (Z)-3-((5-(benzylthio)-4H-1,2,4-triazol-3-yl)imino)-5-haloindolin-2-one derivatives 1a-1l were evaluated in MCF-7 (human breast adenocarcinoma) cell line by MTT assay. Then, the potency of the tested of some synthesized compounds was evaluated on supernatants activities and levels of MMP-2 and MMP-9 by gelatin zymography and ELISA methods. Among the tested compounds, 1j, 1l and 1k had the greatest cytotoxicity against MCF-7 cell line compared to the positive (sunitinib) and negative (DMSO) controls. Moreover, our observations indicated that the compounds 1j, 1l and 1k decreased the supernatants activities of MMP-2 and MMP-9 more than others and all of the tested compounds considerably decreased the supernatants levels of MMP-9. Finally, our findings suggest that the tested derivatives are probably able to inhibit macromolecules like MMPs which have essential role in angiogenesis pathway.

Opioid compound and methamphetamine are commonly used in drug abuse; these can disrupt the normal function of cellular and molecular systems, leading to several events such as oxidative stress, aging, apoptosis, and necrosis. Malondialdehyde (MDA) is the most important biomarker for evaluation of oxidative stress and determination of lipid peroxidation. In this study, 42 drug abusers and 22 healthy persons participated as case and control groups, respectively. MDA in volunteer sera was determined by high-performance liquid chromatography (HPLC) with fluorescence detection after pre-column derivatization using thiobarbituric acid. The analysis was performed on a ODS column by spectrofluorometer detection, operated at excitation of 515 nm and emission of 535 nm. A mixture of phosphate buffer (0.05 M, pH 6.8), containing potassium monobasic phosphate and methanol (60:40, v/v) at a flow rate of 1 ml/min, was used as the mobile phase. The retention time of MDA-TBA was 3.2 min. Our findings showed that the MDA level increased in the opioid and methamphetamine abusers when compared to the control group (P