فهرست مطالب rezvan attari

Chronic myeloid leukemia is one of the most common types of blood cancer that, despite recent advances in cancer treatment, still results in high patient mortality. Due to the side effects of anti-tumor drugs, natural products such as seaweed extract can be more convenient and safer in treating cancer. In this study, the anticancer activity of Sargassum tenerrimum extract was investigated by assessing the rate of apoptosis and expression of the WT1 gene of the K562 chronic myeloid leukemia cell line.

In this study, 250, 500 and 750 mg/mL of methanolic, chloroform and hydroalcoholic extracts were prepared. After the cultivation, the K562 cells were incubated with the extract for 24 hours, followed by observation under an inverted microscope to check the degree of apoptosis. After RNA extraction and cDNA synthesis, expression of WT1 gene was determined by real-time PCR. Results showed that all extracts were concentration-dependent and contributed to the induction of the apoptotic cell line K562. Depending on the concentration and type of various extracts from Sargassum tenerrimum, inhibition of gene expression of the WT1 gene was also observed.

The most effective induction of apoptosis and inhibition of gene expression was developed by 750 mg/ml hydroalcoholic extract and chloroform extract was the least effective.

These results indicate the anti-cancer potential of Sargassum tenerrimum extract on K562 cells by inducing apoptosis as well as inhibiting WT1 gene expression.

Macrobenthos play an important role in aquatic ecosystems because they mineralize, promote, and mix the oxygen flux into the sediment, which recycles the organic matter. Sampling of macrobenthos populations for this study was carried out in the supratidal, mid tidal, low tidal, and subtidal regions of the Dayyer, Ouli, and Kangan stations in the Persian Gulf during both cold and warm seasons. Water parameters such as the temperature, salinity, pH, turbidity, and electrical conductivity were assessed, and were indicative of moderate water quality. Representatives of a total of 31 taxonomic families were identified during both seasons, of which 59 were identified to genus and species. The highest average number of species was observed in the low tidal region of Kangan in the warm season at 16.6 ± 1.2 species, and the lowest number was observed in the supratidal regions of Ouli and Kangan in the cold season at 6.6±2 species. The species diversity, as calculated using the Shannon–Wiener Index (H’), showed a significant difference between sampling seasons and sampling stations, as well as pollution level at the beaches. The species diversity index in Ouli, Dayyer, and Kangan stations also differed between the seasons. In both seasons, the Kangan station showed the highest species diversity while the Dayyer station showed the lowest. These results reveal a rich species diversity of macrobenthos and good water quality at the three beaches in the Persian Gulf. Re-assessment of species diversity during an environmental impact assessment prior to urban development should be further conducted to ensure that the

community is not significantly affected and the ecosystem remains intact.