جستجوی مقالات مرتبط با کلیدواژه « Dunaliella » در نشریات گروه « شیلات »

Biotechnology seeks to find effective stimuli to increase the rate of growth and the content of various biochemical compounds such as lipids and pigments in algae. Temperature is one of the most important physical factors that has a significant impact on the growth and activity of aquatic organisms. Dunaliella is an algae that is categorized as chlorophyll in algae without cell walls, moving, and salty. Dunaliella two-tagged algae are recognized as the most resistant photosynthetic eukaryotic organism. Most microscopic algae produce high levels of lipids under stress conditions. Changing environmental conditions, in addition to affecting the amount of growth and fat production, can also affect the quality of the microalgae fat. In this research Dunaliella algae were cultured in Whalen culture medium after sampling from Uromiye Lake and purification. Experiments were performed with 4 treatments and 6 replicates completely randomized. For the optimal growth of Dunaliella algae, the lightness of 3000 lux was achieved with 12 hours of lightning and 12 hours of darkness and Whalen's culture medium. Counts of algae cells, daily temperature and pH measurements, and fat content in different growth phases. Data were analyzed using SPSS software. According to the results, the growth of algae decreased with increasing temperature and the highest growth was observed at 20 ° C. Also, we found that the highest amount of fat storage was in the droplet at 25 ° C and in the constant phase.

In order to identify the species of green algae Dunaliella , the sampling from coastal waters in Qeshm Island was carried out and algal samples were grown in Johnson medium under sterile conditions. Algal samples were purified via cultivation as single colony on the solid medium containing agar and then single colonies transferred to liquid medium. To identify the genus and species of Dunaliella , taking some morphological characteristics, including the presence or absence of cell wall, flagella, eye spot and shape and cell size, and algae were identified by identification keys of genus and species. For study of the algae response to salinity, algal samples exposed to different concentrations NaCl, and the amount of glycerol produced and the growth rate of the cells was measured. Finally in this study, four species of D u n a li e ll a m a r iti m a , D . Mi n u ti s i m a , D. quartolecta and D . Vi r i d i s based on morphological characteristics (above mented) and also physiological characteristics, such as glycerol production under salinity (1 to 4 M salt), the optimal growth on the 1 or 2 molar NaCl, the color changes of algae from green to orange- red or did not change color of algae at high salt concentrations (4 mM NaCl), were identified