فهرست مطالب

مجله مطالعات علوم پزشکی
سال بیست و هفتم شماره 6 (شهریور 1395)

تاریخ انتشار: 1395/06/28
تعداد عناوین: 10

فارسی
English

انتخاب همه

COMPARISION OF IMPRESS SMEARS AND TISSUE SECTION STAINED WITH DIFFERENT METHODS FOR DIAGNOSING OF PNEUMOCYSTIS CARINII

Khosrow Hazrati Tappeh *, Toohid Yahyapour, Jalil Musavi, Habib Mohammadzadeh, Kambiz Diba, Ali Rahiminejad Pages 449-457

Background and Aims
Pneumocystis carinii is an opportunist extracellular microorganism. The body can defend itself against it in natural conditions. But it can cause an infection in immunodificient conditions such as HIV, Hodgkins and other immunosuppressed diseases. The aim of this study was to apply a comparison of different staining methods such as Methenamine silver, Giemsa, Gram - Weigert, Papanicolaou and Hematoxylin eosin stains in terms of staining times, easiness, cost and availability of material and methods, detection of P. carinii, different forms and capability of this methods in detection of P. carinii in impression slides, and tissue slices of rats lungs.
Materials and Methods
In order to induce of Pneumocystis pneumonia, the Sprague Dawley rats were injected 0.5cc of dexamethasone subcutaneously twice a week over a 10-12 weeks, after induce pneumonia we removed the lungs and made impression slides and pieces of lung was put in 10% formalin to make tissue slides. After that the slides stained by Methenamine silver, Gram Weigert, papanicolaou, Giemsa and Hematoxylin eosin, and compared them in terms of staining time, easiness, cost and availability. In addition, we compared the capability of different methods to detect the microorganism in tissue preparation slices and impression slides.
Results
Giemsa and papanicolaou are capable to stain the intra cyst bodies but cant stain the cysts wall. Methenamine silver and Gram Weigert stain the cysts wall. Giemsa is fastest, cheapest and the most easy method to stain, vs. methenamine silver is expensive, difficult and takes long time to do. Methenamine silver papanicolaou, Gram Weigert can detect P. carinii in both tissue slice and smears but Hematoxylin eosin cant detect either slices or impression slides. Giemsa only detects microorganism in impression slides.
Conclusions
In these staining methods, Papanicolaou and Giemsa have more specificity than Gram Weigert to detect the P. carinii in specimens, because these staining method dont specify the wall of cysts together with yeasts, because of this the wrong diagnosis with Giemsa and Papanicolaou is less than Gram Weigert. Methenamine silver is golden standard method to detect P. carinii but it stains the yeasts like P. carinii because the yeasts are similar to cysts in shape and color when we stain with methenamine silver. Because of this we conclude that Methenamine silver is suitable and useful for demonstration of P. carinii cysts but it is better to be used together with Giemsa, because Giemsa can stain the intra cyst bodies so the diagnosis of pneumocystis pneumonia becomes easy with these methods together.
Keywords: Pneumocystis carinii, Rat, Diagnostic specimens, Different staining methods

Abstract View Paper Original: Persian
EVALUATING THE ANTI-PROLIFERATIVE EFFECT OF 2-METHYL-3-PENTYL-6-METHOXYPRODIGININE IN HEPATOCELLULAR CARCINOMA CELLS

Mohammad Reza Sam *, Reza Abdi Yenkejeh Pages 458-465

Background and Aims
Hepatocellular carcinoma remains often refractory to classic therapies. Therefore, the search for new natural compounds with minimal toxicity is of particular interest in treatment of liver cancer. In this context, it has been shown that 2-methyl-3-pentyl-6-methoxyprodiginine isolated from cell wall of Serratia marcescens has powerful growth inhibitory effects against different kinds of cancer cells. In this study, we investigated the anti-proliferative effect of prodigiosin on the human hepatocellular carcinoma HepG2 cells on a dose-response and time-course basis.
Materials and Methods
Malignant cells were treated with various concentrations of prodigiosin (100, 200, 400 and 600 nM) and incubated for 24 to 72 h. After treatments, proliferation-rates were determined by MTT assay. Following 48 h treatments with indicated concentrations of prodigiosin, cell number and apoptotic rates were also measured by hemocytometer and flow cytometer respectively.
Results
Treatment of cells with increasing concentrations of prodigiosin significantly decreased proliferation-rates in a dose-and time-dependent manner compared to untreated cells. Specifically, Following 72 h treatments with 100, 200, 400 and 600 nM prodigiosin proliferation-rates were found to be %44 ± %2, %33 ± %4, %27 ± %3 and 27% ± %5 for indicated concentrations of prodigiosin respectively. We also found that the cell numbers were decreased in a dose-dependent manner. Specifically, 48 h treatment with 600 nM prodigiosin resulted in 77% decrease in cell number compared to untreated cells. An increase in the number of apoptotic cells (late), ranging from 37 to 97.4 %, was also observed with increasing prodigiosin concentrations.
Conclusion
Prodigiosin decreases proliferation of hepatocellular carcinoma cells with induction of apoptosis in these cells. Therefore, this compound may provide a powerful growth inhibitory agent on proliferation of hepatocellular carcinoma cells.
Keywords: Hepatocellular carcinoma, Prodigiosin, Serratia marsescens, Cell proliferation, Apoptosis

Abstract View Paper Original: Persian
CHANGES IN HRCT SPIROMETRY AND CLINICAL SYMPTOM IN SCLERODERMA PATIENTS TREATMENT WITH INTRAVENOUS CYCLOPHOSPHAMIDE

Mehrzad Hajialilo *, Ali Taghizadieh, Alireza Khabbazi, Amir Ghorbanihaghjo, Mina Asadzadeh Pages 466-475

Background and Aims
Scleroderma is a systemic condition with various manifestations in different organs. Among these organs, the lungs are affected very frequently, and the involvement is progressive and significant. Various medications have been suggested and tested in patients with scleroderma and lung involvement, but their efficacy and safety profile differ and enforce a difficult final decision-making. Since cyclophosphamide is an example of this kind, this study sought to examine the therapeutic effect of this drug in patients with scleroderma and lung disease.
Materials and Methods
A total of 20 patients with scleroderma and lung parenchymal disease received intravenous cyclophosphamide (750 mg/m2) along with gradually tapering prednisolone (20-7.5 mg/day) and azathioprine (2mg/kg) after discontinuation of the former for six consecutive months. The Transition Dyspnea Index (TDI), Forced Vital Capacity (FVC), Total Lung Capacity (TLC), 17 revised Rodnan Score were examined three and six months after starting the treatment.
Results
Six months after treatment, significant changes in FVC (p = 0.55), TLC (p = 0.39), FEV1 (p=0.27), 17 revised Rodnan Score (p=0.98) was not observed. The TDI also did not show significant changes during the six months of treatment (p = 0.79). Based on the type of antibody was not seen significant difference in the amount of TDI.
Conclusion
Administration of intravenous cyclophosphamide in the treatment of scleroderma in patients with pulmonary involvement of our study did not have considerable effects on pulmonary function and clinical manifestations that were similar to other studies in this field.
Keywords: Scleroderma, Lung Disease, Cyclophosphamide

Abstract View Paper Original: Persian
The effect of induced diabetes and its treatment with zinc sulfate & vanadium on reproductive system in rat

Ali Amini, Miss Paria Parto, Namdar Yousufvand* Pages 476-485

Background and Aims
Diabetes mellitus is associated with changes in testicular tissue. Here, the effects of co-administration of zinc and vanadium sulfate on testicular damage in diabetic animals were studied.
Materials and Methods
Moderate diabetic hyperglycemia was induced by injection of streptozotocin (STZ) 40 mg/kg, ip. Five groups of animals were selected (n=5). Group I: As normal group, during the trial period did not receive any drug treatment and consumed tap water during 45 days. Group II: As control diabetic group, received STZ 40mg/kg, ip injection. During the trial period they did not receive any drug treatment and they consumed tap water. The diabetic animals (STZ 40mg/kg, ip) with 500-600 mg/dl blood glucose levels were randomly divided into three groups: Group III: As under treatment 1, used drinkable water containing 1mg/ml of vanadium sulfate Group IV: As under treatment 2, used drinkable water (0.25 mg/ml zinc sulfate) Group V: As under treatment 3, used drinkable water (1mg/ml of vanadium sulfate and 0.25 mg/ml zinc sulfate). Testicular damage were assessed using H & E staining.
Results
Zinc sulfate and vanadium sulfate and their combination have reduced glucose plasma levels in diabetic rats. Vanadium sulfate reduced spermatogenesis and seminiferous tubule diameter in diabetic rats. Seminiferous tubule diameter and spermatogenesis have increased in diabetic rats receiving a combination of oral zinc and vanadium sulfate.
Conclusion
This study demonstrated that the use of zinc sulfate reduced testicular damage and can neutralize the negative effects of vanadium sulfate on testis in diabetic rats.
Keywords: Diabetes, Glucose, Rat, Vanadium, amp, amp, Zinc, sulfate, Testis

Abstract View Paper Original: Persian
MANUFACTURE OF NANO-FIBERS ELECTROSPUN POLY-AMIDOAMINES CONTAINING DOXORUBICIN

Saman Ayoubi, Mohammad Sadegh Hashemzadeh* Pages 486-493

Background and Aims
Doxorubicin sold under the trade names Adriamycin is an anticancer drug that interferes with the growth and spread of cancer cells in the body. The purpose of this study was to produce nano-fibers, polyamide amine (PAMAM) containing Doxorubicin drug using electrospinning technique.
Materials and Methods
This cross-sectional study was conducted in the Faculty of Pharmacy in Tehran University of Medical Sciences, from 2014 to 2015. In the this study, concentration of PAMAM in ethanol, ratio of drug to polymer, Voltage and output current intensity as independent variables, and the diameter of the fibers produced as the dependent variable were selected and studied. Then using the SPSS software, equation of producing nano-fibers were evaluated and the polymer films containing drug by SEM attention of the morphologic, and the diameter of the fibers and the FTIR were studied both physically and chemically.
Results
According to the FT-IR of drug with the polymer After the electrospinning process, it was revealed that neither of these two materials lost their properties during electrospinning, and no chemical change was observed in their structure.
Conclusion
Provided that the solution concentration and the ratio of drug to polymer reduces, defective fibers will be formed, and further reduction of these two parameters will lead to formation of micro and nano particles.
Keywords: Doxorubicin, Poly, amidoamines, Nanofibers, Electrospinning

Abstract View Paper Original: Persian
STUDY OF THE DEMOGRAPHIC AND CLINICAL STATUS, MEDICAL HISTORY LIVING DONOR KIDNEY PRIOR TO DONATION AND RELATED FACTORS WITH DURATION OF HOSPITALIZATION AFTER NEPHRECTOMY IN TRANSPLANT CENTER OF IMAM KHOMEINI HOSPITAL, URMIA (1996 - 2015)

Ehsan Allah Kalteh, Shaker Salarilak *, Ali Taghizadeh Afshari, Hamid Reza Khalkhali Pages 494-502

Background and Aims
The superior results achieved with kidney transplantation from living donors have resulted in an increase in this method of transplantation. But donors need to have a major operative procedure that is associated with morbidity and mortality. Duration of hospitalization after surgery is a measure to express health consequences as well as one of the most important determinants of the cost of the transplant. Therefore, this study aimed to study the demographic status, medical history and clinical living donor kidney prior to donation and related factors the duration of hospitalization after nephrectomy in transplant center of Imam Khomeini Hospital, Urmia.
Materials and Methods
We conducted a retrospective cohort study that was conducted on 1463 living donors who were operated in transplant center of Imam Khomeini Hospital, between 21March 1996 to 19 March 2015. Associations between demographic variables and clinical findings before kidney donation and health history of patients with duration of hospitalization after kidney donation were analyzed using Mann-Whitney U-test
Results
There were 1367 (93.4%) male and 96 (6.6%) female donors. The mean donation age, systolic blood pressure, Diastolic (Nephrectomy before) and after nephrectomy duration of hospitalization were 27.92±5.34 years, 112.12 ±10.5 &71.28±8.89 mmHg and 3.14±0.96 days, respectively. The prevalence of tobacco use, hypertension, hospitalization history and drug was 61.2%, 3%, 27.4% and 5.7%, respectively. Being older than 40 years (p
Conclusion
It is recommended to minimize the duration of hospitalization for donors after surgery, using male gender younger than 40 years old.
Keywords: Kidney, Kidney transplantation, Living donors, Nephrectomy, Hospital stay

Abstract View Paper Original: Persian
Stereological study on the protective effect of Nigella sativa oil on the mice testis tissue following Para-Nonylphenol induced toxicity

Dr Malek Soleimani Mehranjani *, Dr Mohammad Ali Shariatzadeh, Arezoo Solati Pages 503-514

Background and Aims
Para-Nonylphenol (p-NP) is an estrogenic compound with toxic effects on the various organs including testis. The purpose of this investigation was to study the toxic effects of p-NP on the testis tissue of mice and evaluation of the effect of Nigella sativa oil (NSO) against this toxicity
Materials and Methods
This study was conducted on 24 adult male NMRI mice (32±3 g) that were divided into 4 groups (n=6), control, NSO (5mg/kg/day), p-NP (250mg/kg/day) and p-NP NSO, and treated orally for 34 days. Finally, mice were sacrificed, their right testis were taken out, fixed, sectioned, processed and stained using Heidenhain̕ & azan method. Testicular tissue sections were evaluated using stereological method. Lipid peroxidation index, malondialdehyde (MDA) level, was also measured in serum of mice. The data were statistically analyzed using one way ANOVA and difference means was considered significant at P
Results
A significant decrease in the diameter (P
Conclusion
NSO has a protective effect on the p-NP toxicity in the mice testis therefore it can be suggested as a raputic supplement in preventing the adverse effects of p-NP exposure in industrial zones.
Keywords: Stereology, Testis, Para, nonylphenol, Nigella sativa oil, Mice

Abstract View Paper Original: Persian

علمی مصوب

مجله مطالعات علوم پزشکی

Journal of Medical Science Studies

ماهنامه پزشکی به زبان فارسی و انگلیسی

آخرین شماره | آرشیو

ISSN: 1027-3727 eISSN: 2008-4153

صاحب امتیاز:

دانشگاه علوم پزشکی ارومیه

مدیر مسئول و سردبیر:

دکتر عیسی عبدی راد

تلفن نشریه: ۰۴۴-۳۱۹۳۷۳۳۲

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فهرست مطالب

مجله مطالعات علوم پزشکی سال بیست و هفتم شماره 6 (شهریور 1395)

مجله مطالعات علوم پزشکی

Journal of Medical Science Studies

مجله مطالعات علوم پزشکی
سال بیست و هفتم شماره 6 (شهریور 1395)