Researcher Bulletin of Medical Sciences
Volume:20 Issue: 6, 2016

Probiotics are beneficial organisms that have therapeutic effect within microbial flora. The aim of this research was to determine the antagonistic properties of Lactobacillus plantarum, fermentum and rhamnosus isolated from healthy baby stools on growth of Acinetobacter baumannii and Pseudomonas aeruginosa of nosocomial origin.
In total 105 samples of nosocomial infection were collected and processed for bacterial isolation, identification and antimicrobial susceptibility testing, by means of standard biochemical tests and following standard bacteriological techniques disk diffusion method. The antagonistic effect of lactic acid bacteria carried out by Well method.
From 105 isolates, 29 were identified as non-fermentative including 17 Acinetobacter baumannii and 12 Pseudomonas aeruginosa respectively. Most of Acinetobacter baumannii isolates showed high resistance to most tested antibiotics except ampicillin sulbactam. Pseudomonas aeruginosa isolates showed resistance to ampicillin sulbactum and gentamycin and sensitive to meropenem. The isolated lactobacilli such as Lactobacillus plantarum, fermentum and rhamnosus had substantial antagonistic effects against tested pathogenic bacteria causing nosocomial infections.
‍ The results of this study showed that Lactobacillus plantarum, fermentum and rhamnosus had considerable effect on preventing the growth of fermentative and non-fermentative pathogenic bacteria.

In this study, we aimed to investigate whether a diet rich in high-sulforaphane broccoli sprouts powder (BSP) could attenuate metabolic abnormalities induced by high-fructose corn syrup (HFCS).
Forty-eight male wistar rats with similar average body weight (253±23g) were randomly allocated into four groups: Baseline control, control (C) (normal diet), fructose (F) (normal diet water containing 30% fructose), and fructose BSP (F) (diet with 5% BSP water containing 30% fructose), and followed for 8 weeks. Biochemical measurements including fasting serum triglyceride levels, total cholesterol, HDL-C, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, and aspartate aminotransferase were assessed at baseline, and the fourth and eighth weeks.
The mean change of body weight was 31.5%, 31.4% and 18.3% in the C, F, and F groups, respectively; the mean weight gain of group F was lower than those of the other groups (p=0.009). A significant increasing trend was observed in serum total cholesterol, triglycerides and LDL-C levels in group F during the intervention (p‍ High-sulforaphane broccoli sprouts could have favorable effects against metabolic disorders-induced by a high fructose diet.

Probiotics are beneficial organisms that have therapeutic effect within microbial flora. The aim of this research was to determine the antagonistic properties of Lactobacillus plantarum, fermentum and rhamnosus isolated from healthy baby stools on growth of Acinetobacter baumannii and Pseudomonas aeruginosa of nosocomial origin.
In total 105 samples of nosocomial infection were collected and processed for bacterial isolation, identification and antimicrobial susceptibility testing, by means of standard biochemical tests and following standard bacteriological techniques disk diffusion method. The antagonistic effect of lactic acid bacteria carried out by Well method.
From 105 isolates, 29 were identified as non-fermentative including 17 Acinetobacter baumannii and 12 Pseudomonas aeruginosa respectively. Most of Acinetobacter baumannii isolates showed high resistance to most tested antibiotics except ampicillin sulbactam. Pseudomonas aeruginosa isolates showed resistance to ampicillin sulbactum and gentamycin and sensitive to meropenem. The isolated lactobacilli such as Lactobacillus plantarum, fermentum and rhamnosus had substantial antagonistic effects against tested pathogenic bacteria causing nosocomial infections.
‍ The results of this study showed that Lactobacillus plantarum, fermentum and rhamnosus had considerable effect on preventing the growth of fermentative and non-fermentative pathogenic bacteria.

According to known complications associated with cesarean section and rising trend of the section up to before the implementation of health sector evolution plan, the purpose of the study was to examine the effects of the plan on changes of indications and whether the aims of plan leading to reduce cesarean or not.
This descriptive study was conducted by using available information. According to the documentation, indications for cesarean section was set on the basis of Health Deputy’s check list that includes: elective cesarean, fetal distress, previous cesarean section, cephalopelvic disproportion, malpresentation, failure to progress, multiple pregnancies, post term pregnancy, placenta previa, abruption placenta, meconium stains or due to other causes, which are unknown. Pregnant women admitted to hospital only on the basis of the indications for cesarean section and in two time periods, before and after the project implementation of the health system were studied. Information obtained were analyzed by using SPSS-18 software t-test and chi-square.
The results showed that pregnant women in 2014, indications such as previous cesarean section and unknown causes increase with p-value ‍ According to the results of the project and the total overall health system in reducing cesarean indications for cesarean section, it seems that with persistence and good performance of the scientific objectives, bringing the rates closer to international standards.

There is growing evidence that the relationship between coping ways with stress and coronary heart disease. Therefore, the aim of this study was to examine the relation between coping ways with stress and systolic and diastolic blood pressure in coronary heart disease.
Survey population, all patients were with CHD who referred to Tehran Shahid Rajaie Heart Hospital. Forty-four patients with CHD admitted to different parts of hospital were selected from among eligible patients. In this study the coping ways questionnaire Lazarus and Folkman was used and set of systolic and diastolic blood pressure (Omron). For statistical analysis, research data was used. Indicators and descriptive statistics and Pearson correlation and multiple regression analysis with stepwise method were used.
The results showed there are positive and significant correlation between emotional focused coping ways and systolic and diastolic blood pressure and also negative and significant correlation between problem focused coping ways and systolic and diastolic blood pressure and also coefficient of avoiding coping way according to the t-test indicate that among 8 of coping ways only this variable with 99% confidence can be predicted to the changes related to systolic and diastolic blood pressure.
Results showed that the increased use of emotional focused coping ways lead to increases in levels of systolic and diastolic blood pressure and increased in the use of problem focused coping ways lead to decrease in levels of systolic and diastolic blood pressure.

Long-term opioid consumption produces tolerance to its antinociceptive effect. There are some documents about nNOS in morphine tolerance but little is known about the reduction of antinociceptive effect of local anesthetic drugs in morphine tolerance. Therefore, this study was to consider the role of nNOS protein on reduction of the analgesia of anesthetic drugs in morphine tolerance.
Male wistar rats weighning 200-250 grams were used. The spinal canola was implanted. Subsequently, interathecal morphine 5μg/kg, 3 times a day, for 5 days was injected. To investigate tolerance induction, formalin test was performed at 5thday. Twenty minute after the last morphine injection at 5th day, bupivacaine 5μg/kg was injected interathecally, and then formaline test was performed. In another series of experiments, 20 minutes after last morphine injection at 5th day, bupivacaine 5μg/kg injected and then after 20 minutes, nNOS inhibitor (7-NI) 10 μg/μl was injected and after 60 minutes, formalin test was perfumed.
The results showed that repeated injection of morphine induced tolerance. Bupivacaine analgesia in morphine tolerance reduced. 7-NI the nNOS inhibitor drug could reduce the tolerance to analgesic effect of morphine along bupivacaine in compare to morphine tolerance.
The results showed that nNOS has a crucial role in reduction of analgesic effect of bupivacaine in morphine tolerance.

One of the most important and underlying marital problems in couples is marital disillusionment. The purpose of this study was to evaluate the validity and factor structure of the scale of the Marital Disillusionment Scale.
A sample of 188 teachers was selected with convenience sampling of students of Karaj. To calculate the convergent and divergent validity, attitudes toward infidelity scale, Kensas marital satisfaction scale, attachment styles & marital forgiveness were used. In order to assess the first order confirmatory factor of the marital disillusionment scale, the weighted lowest squares (WLS) and to assess the adequacy of the model to the data, the parameters of RMR, RMSEA, CFI, AGFI, GFI, c2, c2/df, and Dc2 were used.
Factor structure of the marital disillusionment scale was confirmed with using confirmatory factor analysis. Cronbach’s alpha and retest coefficients were 92.0 and 85.0 respectively. Correlation analysis showed that the marital disillusionment scale has a significant positive correlation with attitudes toward infidelity (p‍ First-order factor structure of "marital disillusionment scale" showed better fitness with the observed data. Confirmatory factor structure, reliability and validity of the marital disillusionment scale for research applications and clinical diagnoses were acceptable.

The aim of this study was determination the prevalence of Klebsiella pneumonia carbapenamase (KPC) and Metallo-β-lactamases (MBL) genes among P. aeruginosa isolates from cystic fibrosis (CF) and burn patients.
This descriptive study was carried on the 70 and 30 isolates of P. aeruginosa from wound of burn patients of Shahid Motahari hospital and sputum of CF patients of Mofid Pediatric hospital of Tehran during 2013, respectively. Antibiotic susceptibility test (AST) and minimal inhibitory concentration (MIC) by microdilution both were performed based on CLSI guidelines. Combination Disk Diffusion Test (CDDT) and Modified Hodge Test (MHT) detected MBL and KPC production, respectively. PCR and further sequencing determined frequency of IMP, VIM and KPC genes.
Resistant profile of P. aeruginosa isolates from wound of burn and sputum of CF patients to tested antibiotics by AST were as follow: 60 (85.71%) vs. 1 (3.3%) to imipenem, 58 (82.85%) vs. 11 (36.6%) to meropenem, (81.67%) vs. 13 (43.3%) to gentamicin, 56 (79.32%) vs. 8 (26.6%) to aztreonam, 56 (79.32%) vs. 5 (16.6%) ciprofloxacin, 55 (78.21%) vs. 5 (16.6%) to cefepime, 54 (77.12%) vs. 10 (33.3%) to ceftazidime, 52 (74.15%) vs. 3 (10%) to piperacillin. By CDDT, (13/30) 43.3% and (46/70) 51.42% of CF and burn P. aeruginosa isolates were MBL producer, respectively. No P. aeruginosa was identified as KPC-producer by MHT. The prevalence of IMP, VIM and KPC genes among bacterial isolates of burn patients were 7(10%), 0 (0%) and 0 (0%). None of the mentioned genes were detected among CF isolates.
Because of the high incidence of MBL P. aeruginosa producer among both burn and CF patients, MBL and carbapenemase identification is important for prevention strategies of infections.