فهرست مطالب

Journal of Kerman University of Medical Sciences
Volume:25 Issue: 2, Mar-Apr 2018

  • تاریخ انتشار: 1397/01/20
  • تعداد عناوین: 10
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  • Roghieh Bayrami, Mahdi Yousefi, Hossein Ebrahimipour* Pages 101-103
  • Mohsen Khalili, Farhood Ghazi Feruozsalari *, Arezoo Naeimabadi, Jaber Atashbar, Negin Ballar, Alireza Raji- Amirhasani, Yusef Babakhani Omid, Davoud Kamali Pages 104-117
    Introduction
    Curcumin, a natural antioxidant, has anti-inflammatory and protective effect on a large number of diseases like cancers and hepatic disorders in oxidative stress conditions by collecting free oxygen radicals and increasing intracellular glutathione. The aim of this study was to determine the effects of curcumin on the level of IL-17 and IL-10 cytokines in intrahepatic and extrahepatic liver injuries
    Methods
    A total of 72 male Wistar rats were randomly divided into two (A, B) categories, each of which was divided into 4 groups. A: One group as a control-sham group received distilled water as an acetaminophen vehicle and the other three groups received acetaminophen (500mg/kg IP). The third group received curcumin, and the fourth group was administrated curcumin vehicle. B: one group underwent Bile Duct Ligation (BDL), and another group received curcumin by gavage for seven days. The third group received distilled water as a curcumin vehicle and the fourth group was considered the sham group. Animals were sacrificed 48 hours after administration of acetaminophen under anesthesia with ketamine xylazine. After that, liver tissue samples were taken for laboratory tests. Cytokines were measured by ELISA method.
    Results
    Levels of IL-17 and IL-10 in the liver tissue in groups A and BDL increased significantly, and in the Curcumin (CMN) group, decreased significantly in both in- and out-liver injury. Also, the body weight in the curcumin-treated groups showed a significant increase both in intrahepatic and extrahepatic injuries.
    Conclusion
    Our data suggest that curcumin undermines inflammation and damage to the inside and outside of the liver, but these findings need to be further investigated
    Keywords: Bile duct ligation Interleukin, 10 Interleukin, 17 Rat Curcumin Cholestasis
  • Faranak Firoozfar, Seyed Hassan Moosa-Kazemi, Javad Rafinejad, Abedin Saghafipour*, Mojgan Baniardalani, Mustapha Ahmed Yusuf, Fatemeh Hosseini, Akbar Biglarian, Gidiglo Godwin Nutifafa, Abbas Bahrami Pages 118-127
    Background
    The larvae of Lucilia sericata are efficiently and widely used in maggot therapy. The aim of this study was to investigate some environmental factors
    that influence the mass rearing of Lucilia sericata as the most suitable candidates for maggot therapy in Iran.
    Methods
    This cross-sectional study was conducted in flies breeding insectarium of Tehran University of Medical Sciences. The best temperature for embryonic period and hatching was 27 °C with relative humidity of 80% and 16:8 light-dark periods. At the insectarium, food, water and a nest for laying eggs were provided for the flies and after oviposition, the eggs were transferred to a new rearing place and identification keys were used to identify the specimen. Four factors (temperature, humidity, photoperiod and diet) were studied for the maintenance and mass rearing of Lucilia sericata larvae under laboratory condition.
    Results
    The best temperature for embryonic period was 27 °C (P
    Conclusion
    In in-vitro condition, although temperature, humidity, light-dark period and diet had an effect on the growth of L. sericata, however, temperature and relative humidity were found to have more influence in the development of L. sericata larva.
    Keywords: Lucilia sericata, temperature, Relative humidity, Photo period, Iran
  • Fatemeh Mirzaei, Samira Azimi*, Ghazal Mansouri Pages 128-136
    Background
    Women with preterm labor put in an arrest phase by tocolytic therapy, are at increased risk of recurrent preterm labor. The aim of this study is to evaluate vaginal progesterone suppositories as compared to intramuscular type in order to prevent preterm labor in women with episodes of threatened preterm labor.
    Methods
    This prospective longitudinal study was conducted on 108 pregnant
    women who were presented with symptoms of threatened preterm labor and went on tocolytic therapy in order to prevent uterine activity. Their gestational age (GA) ranged between 24-34 complete weeks and mothers were at risk of preterm delivery. After acute phase control and delivery supression, 400mg of prophylactic vaginal suppository was prescribed each day in the first intervention group and 250 mg of intra-muscular 17-alpha hydroxyprogesterone caproate was injected once a week in the second intervention group. Treatment continued in both groups up to 36 weeks of gestational age or until delivery.
    Results
    The prevalence of preterm delivery was significantly higher in patients receiving intra-muscular progesterone (55.6% vs. 37%) (P = 0.05). Moreover, the birth weight in the intramuscular progesterone group was significantly lower than the other group (2685.18 g vs. 2999.25 g) (P = 0/02). First and fifth minute Apgar score were also significantly lower in this group than the vaginal progesterone suppository group (P
    Conclusion
    The results of this study suggest that vaginal progesterone suppository decreases the rate of preterm delivery while it also improves the newborn outcome at a higher rate compared to intra-muscular 17-alpha-hydroxyprogesterone caproate.
    Keywords: Preterm delivery, Vaginal progesterone suppository, 17, alpha hydroxyprogesterone caproate
  • Mohammadreza Mirzaei*, Shima Khajouee Ravari, Gholamhosein Hasanshahi, Mehdi Mahmoodi, Rahim Heidari Barchi Nezhad, Fatemeh Asadi Pages 137-146
    Background
    OCT4B1, a variant of OCT4, is expressed in both cancer cells and tissues. This variant has a main role in the regulation of both apoptotic and stress (heat-shock proteins) pathways. The aim of this study was to investigate the effects of OCT4B1 silencing on the expression of small heat-shock proteins (sHSPs)in three human tumor cell lines.
    Methods
    AGS (gastric adenocarcinoma), 5637 (bladder tumor) and U-87MG (brain tumor) cell lines were transfected with specific OCT4B1 siRNA (test group) and scramble siRNA (control group), using siRNA and Lipofectamine. Real-Time PCR Array technique was applied and the fold changes were calculated using RT2 Profiler PCR Array Data Analysis version 3.5.
    Results
    It was revealed that HSPB1, HSPB6 and HSPE1 were down-regulated in all three studied tumor cell lines and HSPB2, HSPB7 and HSPB8 were down-regulated in two of three studied tumor cell lines (AGS and 5637). It was also revealed that HSPB3 was down-regulated in 5637 cell line and up-regulated in AGS and U87MG cell lines.
    Conclusion
    According to the results, it may be concluded that there is a direct relationship between OCT4B1 and sHSPs gene family expression. Thus, suppression of OCT4B1 may be considered in cancer therapy/research.
    Keywords: OCT4B1, Small HSPs, Tumor cell lines
  • Ghasem Yadegarfar *, Hanieh Pourmohammadbagher, Mohammad Mehdi Amin Pages 147-152
    Introduction
    Shigellosis is a contagious disease with acute symptoms. Delay in detecting the disease transmission way would lead to rapid spread among the community, like what happened in Isfahan Provincein April 2015. The study aimed at evaluating the Shigella bacteria transmission medium in the outbreak that occurred in Isfahan Province, Iran.
    Methods
    Due to evidences reported by the university health deputy, the beginning of the Isfahan shigellosis outbreak was on April 17th, and the first records of all 8600 cases were reported on April 18th. Through the interviews with Shahreza's hospitalized cases, a wedding ceremony held on April 16th with 67 guests, most of whom were affected by diarrhea. Therefore, a cross-sectional study was designed to report, overall, the Epidemic of Shigellosis and a historical cohort in order to investigate the causes of the diarrhea based on wedding ceremony data. The guest list and their phone numbers were collected from the ceremony host. Data were collected through a phone call interview.
    Data analyses were done by STATA13. The relative risk was calculated with 95% confidence interval using logistic regression.
    Results
    The response rate of the guests following up was 72% (48 of 67 guests), with 33 individuals suffering from diarrhea and 30 having eaten vegetables. Patients were from both sexes and all age groups. The relative risk of diarrhea for vegetable consumers was 4.1 (95% CI: 1.5 – 11.3, p=0.006).
    Conclusion
    Findings have provided strong evidences against Shigella contaminated vegetables as a risk factor and transmission agent for individuals who got diarrhea in Shigellosis outbreak of Isfahan Province in April 2015
    Keywords: Shigella, Epidemics, Transmission, Iran
  • Fatemeh Masoumipour, Mehdi Hassanshahian*, Tayebeh Jafarinasab Pages 153-163
    Background
    Microbial biofilms are responsible for many human infections and increase of antibiotic resistant bacteria. Therefore, finding an efficient way to prevent infection and biofilm formation of bacteria is essential. Medicinal plants are among suitable candidates to inhibit biofilm formation of bacteria. The aim of this study was to evaluate the antimicrobial effects of Trachyspermum, Thymus and Pistachio (T.T.P) combined extracts (methanolic and ethanolic) against six pathogenic bacteria in planktonic and biofilm forms.
    Methods
    The antibacterial activities of T.T.P combined extracts against planktonic form of pathogenic bacteria were evaluated by disc diffusion and macro-broth dilution methods. Also, anti-biofilm activity of the combined extracts was evaluated by microtiter plate method.
    Results
    The results of disc diffusion analysis (MBC and MIC) showed that both methanolic and ethanolic extracts of T.T.P combined extracts were efficient for inhibition of planktonic forms of bacteria, although ethanol extract was more effective compared to the methanolic extract. The T.T.P combined extracts had inhibitory effect against biofilm formation of pathogenic bacteria. Also, these extracts efficiently demolished biofilm formation and prevent metabolic activity of bacteria in biofilm structures. The concentration of each extract had a direct relationship with its inhibitory effect. The maximum inhibitory effects of T.T.P combined extracts on biofilm formation, demolish of biofilm structure and inhibition of the metabolic activity of bacteria in the biofilm were 95.3% for P. aeruginosa, 97.2% for P. aeruginosa and 81.16% for E. coli.
    Conclusion
    The results of this research demonstrated that T.T.P combined extracts can be used as an alternative component with inhibitory effect against pathogenic bacteria in planktonic and biofilm forms.
    Keywords: Antibiotic resistant, Antibacterial activity, Biofilm, Medicinal plants
  • Sepideh Ansari, Majid Soltani*, Negin Moradi, Hossein Rezai, Latifi Seyed Mahmoud, Nastaran Majdinasab Pages 164-174
    Background
    The first purpose of this study was to investigate the rate of oral and laryngeal Diadochokinesis (DDK) in Multiple Sclerosis (MS) patients in comparison with healthy people. The second goal was to determine if DDK rate has any relationship with the disease progression.
    Methods
    In this cross-sectional study, two groups were enrolled: MS patients (n=31) and healthy subjects (n=14) . The samples of DDK tasks were collected. The scores of Expanded Disability Status Scale (EDSS) and duration of disease were considered as the indices of disease progression.
    Results
    There were significant differences between the two groups in the rates of all DDK tasks (P≤0.001). Significant correlations were found between laryngeal DDK tasks and disease progression (r = - 0.488, r = -0.396, r = -0.444, r = - 0.667, P ≤ 0.027). Two oral DDK tasks were in correlation with EDSS and the disease duration (r = -0.403, r = -0.446, r =-0.521, r = -0.465, p≤0.025). There were high correlations between functional systems of EDSS (pyramidal, cerebellar and brainstem) and DDK tasks (r = - 0.448, r = -452, r = -0.458, r =-0.379, P ≤ 0.036).
    Conclusions
    In the present study, poor performance in DDK tasks provided evidence for insufficient motor control over related speech subsystems in MS. These findings suggest that DDK rate is a sensitive beneficial speech motor control assessment in MS. Moreover, DDK tasks might be introduced as additional prognostic parameters for detecting disease progression and evaluating treatment achievements.
    Keywords: Speech motor control, Multiple Sclerosis, Diadochokinesis
  • Soudeh Ghafouri-Fard, Mohammad Amin Hejazi, Davoud Afshar, Abolfazl Barzegari, Solat Eslami* Pages 175-186
    Background
    Traditional dairy products are the main sources for probiotic bacteria. This study aimed to isolate and characterize the potentially probiotic Lactobacillus strains isolated from traditional dairy products in Iran.
    Methods
    Microbial population of each dairy product was enriched and screened for acid- and bile- resistant strains. The isolates were primarily characterized by conventional phenotypic and biochemical tests. The species were finally identified using restriction analysis and 16S rDNA sequencing. In addition, the antibacterial activity of the four most common species was evaluated against seven gastrointestinal pathogens.
    Results
    From a total of nine yogurt and cheese samples, 17 acid-resistant Lactobacillus strains were isolated (survival>50% in PBS buffer, pH 2.5 for 3 h). Eight of them also showed high tolerance to bile. Also, the superiority of molecular techniques over phenotypic tests for effective identification of species was observed. Biochemical tests were useful in combination with 16S-amplified ribosomal DNA restriction analysis (ARDRA) technique to discriminate the isolates at species level.
    Conclusion
    16S–ARDRA in combination with biochemical tests is suggested as a potentially useful method for accurate identification of Lactobacillus species where the 16S-rDNA sequencing could not discriminate between two closely related species. Considering the acid and bile resistance capacity of isolated strains, traditional dairy products may be used as important sources of probiotic strains
    Keywords: Dairy products, Probiotics, Lactobacillus, Molecular techniques, 16S, ARDRA
  • Maryam Hami*, Zahra Amjadi, Natasha Dolatabadi, Fatemeh Tara Pages 187-190
    Pregnancy in end stage renal disease women who are on Hemodialysis has been considered a challenging event for both mother and fetus. Generally, outcomes of pregnancy in patients with end-stage renal disease (ESRD) have been considered to be extremely poor, and pregnancy in this group of patients is rather scarce. The frequency of conception is ranged from 0.3% per year in Belgium to 1.4% per year in Saudi Arabia. Based on data, about 50% of these conceptions are lead to surviving infant finally. Although there is higher prevalence of adverse effects of pregnancy on maternal and fetal outcome in these groups of patients, they can be managed by tight control of patient condition and intensive dialysis. Suitable pharmacological strategies also help to achieve success. We present a young woman with frequent unsuccessful conception in a long duration dialysis who finally had a successful pregnancy with a surviving infant in our center
    Keywords: Hemodialysis, Pregnancy, Chronic kidney disease