نشریه ارمغان دانش
سال بیست و یکم شماره 4 (پیاپی 111، تیر 1395)

Eotaxin, as a pro-inflammatory adipokine is secreted from adipose tissue, which increases its secretion in obesity, and also played a key role in the pathogenesis of asthma. The aim of this study was to investigate the effects of eight weeks’ progressive resistance training on serum eotaxin, non-HDL-c, insulin resistance and some physiological variables in overweight and obese men.
In this quasi-experimental study, 20 healthy overweight and obese male (22.75±1.29 yr, 89.22±13.08 kg, 28.36 ±3.98 kg/m²) volunteered and randomly assigned into training (n =10) and control (n =10) groups. Progressive resistance training was carried out for eight weeks (4 d/wk, 60 min/d). Blood samples were taken before, and 48 hours after the last training session to evaluate serum variables. Eotaxin serum concentration were measured by sandwich ELISA method using a special kit manufacturing by Germany ZellBio company. Dependent and independent t-test and Pearson correlation was employed for data analysis. The criterion of statistical significance was p Independent t-test results showed that after eight weeks of progressive resistance training, in the experimental group compared with the control group, the eotaxin serum concentration changes were not significant (p=0.65), but the amounts of non-HDL-c (p=0.024), triglyceride (p=0.017) and total cholesterol (p=0.032) were significantly decreased. Changes in other variables, such as insulin resistance index, body weight, body fat percentage, body mass index, waist to hip ratio and maximum oxygen consumption was not significant (p>0.05). also dependent t-test results showed that after eight weeks of progressive resistance training, change all the variables in the experimental group (except LDL, HDL and insulin) were significant (p0.05.
It seems that eight weeks of progressive resistance training, cannot have a significant effect on eotaxin serum and insulin resistance, even though significantly reduced non-HDL-c, triglycerides and cholesterol as cardiovascular risk factors. Probably, it is more useful with more intensity and volume. To achieve more accurate results, more studies are needed in this area.

Medicinal herbs with natural materials can be effective and have fewer side effects than drugs with chemical composition in reducing withdrawal symptoms and may have effective treatment of addiction. In recent years, anti-inflammatory, analgesic and anti-anxiety effects of Dill have been reported. The purpose of this study was to determine the effect of Dill ethanol extract on some withdrawal syndrome behaviors such as jumping, standing, climbing in male mice addicted to morphine.
This experimental study was conducted in the first half of year 2014. In this study, 21 male NMRI mice with weighting 25 to 30 g were used. Animals were divided into three groups: Group1 as control- animals of this group were considered as receiving saline naloxone (SN), Group 2 - animals of this group were considered as receiving morphine saline naloxone (MSN), Group3 - animals of this group were considered as receiving morphine Dill ethanolic extract naloxone (MDN). At the same time with each injection of morphine was performed once injection of Dill extract. In order to induce dependency and addiction to morphine animals received increasable doses of morphine at ten times for four days(10 , 20, 40mg/kg in 1 to 3 days respectively and 40mg/kg in 4th day). In order to induce withdrawal, two hours after the last morphine injection, naloxone was injected with dose 5 mg/kg intraperitoneally. Then, number of jumping and standing on two feet and climbing the walls of the container were counted for 30 min. Data were analyzed by using statistical Graphpad prism 5 software.
The results show that injection of Dill ethanol extract reduces the number of jump (jumping) that was induced by naloxone injection significantly (p The findings suggest that the Dill ethanolic extract can decrease in the Naloxone-induced jumping number and therefore, Dill ethanolic extract may have an impact in reducing of withdrawal induction by morphine.

Esophageal cancer is the eighth most common cancer worldwide. This form of cancer is the second most prevalent cancer in Iran. Annually, esophageal cancer makes up 3.2% of all new cancer cases. Altered expression of stem cells self-renewal genes is of great importance in the tumorigenesis. The aim of this study was to compare TBX3 gene expression in tumoral samples of patients with squamous esophageal cancer and normal tissue samples of healthy individuals.
Totally, 40 FFPE tumoral and normal tissue samples were collected from Imam Khomeini hospital of Ahwaz. After extracting RNA from FFPE tissues and synthesizing cDNA, expression levels of TBX3 and GUSB (as internal control) genes were relatively quantified using qRT-PCR.
The results of the qRT-PCR analyses showed that the average relative gene expression in tumor samples was higher than normal samples. Furthermore, TBX3 gene expression exhibited greater expression in the tumor samples of the patients in the 26-50 years age range in compared to the 51-81 years age range
According to the cancer stem cell theory, many tumors originate from stem cells leading to the inhibition of apoptosis and resistance to chemotherapy. Considering the role of TBX3 gene in self-renewal of stem cells and its increased expression in patients with squamous esophageal cancer, it seems that TBX3 gene might be used as a biomarker in the diagnosis and prognosis of esophageal cancer in fu

Urinary tract infections are the most common type of nosocomial infection. Escherichia coli (E. coli) is the most common cause of urinary tract infections (UTIs) in community and clinic. The treatment of these infections is often difficult due to several virulence factors and drug resistance. Adhesions, enzymes and toxins are important virulence factors which promote colonization and pathogenicity of this organism. In this study, frequency of hemolysin and cytotoxic necrotizing factor-encoding genes and antimicrobial susceptibility were evaluated in E. coli isolates collected from urine samples in educational hospital of Qazvin.
In this cross-sectional descriptive study between 2012 and 2013, 126 E. coli isolates were collected from urine samples in educational hospital of Qazvin. All isolates were identified using standard microbiological and biochemical techniques. Antimicrobial susceptibility was further evaluated by Kirby-Baure method according to Clinical and Laboratory Standards Institute guideline. Frequency of hly and cnf genes was determined using specific primers by PCR and sequencing methods. Statistical analysis was performed for descriptive statistics including frequencies and cross tabulation of microbiological, clinical, and demographic characteristics using the computer software program SPSS version 18.
In total, 49 (38.9%) isolates showed the multidrug resistant pattern in which the highest susceptibility rate was shown to amikacin (91.3%) and imipenem (88.9%) and the resistance rate was reported against cefotaxime (83.3%) and ceftazidime (73%), respectively. PCR assay showed that 40 (31.7%) and 24 isolates (19%) were positive for the presence of hly and cnf genes, respectively. Hly and cnf-positive isolates were mostly obtained from patients admitted in internal medicine wards.
The findings of this study show multidrug resistant isolates and a considerable rate of hemolysin and cytotoxic necrotizing virulence factors in E.coli collected from urine specimens in hospitalized patients in Qazvin, emphasizing the importance of these virulence factors in infection prevention and control strategies.

Background & aim: One of the strategies to improve the therapeutic gene is targeting gene therapy. A method which can be considered, is adding code sequences peptide or protein with high tendency to target cells and secreting the therapeutic gene encodes a protein. However, evaluating the effectiveness of such changes in the targeted cell binding protein gene product with the usual therapeutic methods produced in prokaryotic system is directly impossible. The purpose of this study was to evaluate the design methods of cell adhesion proteins based on ELISA usable in-vitro in gene therapy.
In order to target the therapeutic gene Mda-7 by using genetic engineering, peptide coding sequence RGD4C with the tendency to cancerous cell surface integrin were inserted shortly after the artificial signal peptide sequence and the N-terminal coding region of the protein. Then, the modified and unmodified cDNA eukaryotic expression vector pCDNA3.1 were matched. Vectors were transfected in HEK-293 cell line. Then Mda-7 secreted expression levels were measured in cell culture by ELISA. After adjusting the protein concentration of Mda-7 and RGD.Mda-7, in cells transfected media, they were used as a source of protein. Reduce the concentration of these genes was assessed two hours after exposure to the integrin cell lines with HepG2, M21 and lacking integrin Saos-2 were also determined by ELISA. The present study was conducted three times independently. Data were analyzed using t-test.
Statistical analysis of the results suggested that the gene product of the gene product RGD.Mda-7 and Mda-7 to connect to HepG2 cells and M21 were more likely to have integrin. While binding to the cell lines of Saos-2, no significant difference were observed.
It seems the present ELISA based method was a suitabl e strategy for cell attachment assay in gene therapy research.

the aim this study was compared the executive function and difficulties in emotion regulation in addicted with upper and lower borderline personality traits.
the current study is kind of causal – comparative. The study population, was included all men substance use druge which referred to treatment center of khoy city in 2013. 80 people addicted,were selected through available sampling method and by borderline personality traits questionnaire (stb) in two groups of 40 people through drug abusers with borderline traits were high and low. Thus the wisconsin card sorting test and the scale of the difficulties in emotion regulation were performed on the two groups. Data in spss 20 program by statistical method multivariate analysis of variance (manova) and lsd test were analysed.
the results indicated that addicts with high borderline traits than to addicts with lower borderline traits in wisconsin card sorting test has lower performance and higher total number of errors[p=0/002] and error perseveration[p=0/010]. Also addicts with high borderline traits, have higher scores on the difficulties in emotion regulation vatiable [p=0/002] than to addicts with lower borderline traits.
this study showed that drug abusers with high borderline traits, the poorer performance in executive functioning and higher levels of difficulties in emotion regulation which this could be due to the influence of drug dependency on their neurological function which leading to poorer performance this indivitual than addicts with lower borderline traits.

Background and This study aimed to determine predictive factors of a needs assessment and prioritization of interventions to prevent brucellosis using the Precede - Proceed Model in Isfahan rural areas in 2015.
This study was a cross-sectional study (needs assessment by Delphi method) that conducted in 402 people 15 years and older in 20 villages of 44 villages that random selection in the rural Regions of Tiran and Karvan county in of Isfahan Province. The data collected through questionnaires and Self-made checklist based on the Precede-Proceed Model that content and face validity approved by experts and pilot projects. Data collected by the survey techniques agreement using chi-square and t tests analyzed by use of spss18 software.
Findings: This study showed that participants awareness of the possibility of transmission of brucellosis from skin and breathing dust as well as the use of personal protective equipment at the time of contact with animals is not appropriate, increase access ranchers to veterinary services and vaccination of livestock herders in rural areas Help build homes for the animals in the backcountry villages and migration out of the rural areas of the wishes of the population .This study showed that between knowledge, attitude and performance of the participants with their educational level is a significant association (p≤0.05), Also, between enabling factors, reinforcing factors and skill and practice in the prevention of brucellosis and having cattle was significant relationship. (P≤0.05)
Assess the needs before implementing any intervention in the prevention of brucellosis can help to researchers, planners and policy makers to assist in the design and implementation of interventions as well as supplying educational materials situation and priorities of influential Disease Control and Prevention consider put into targeted action that will have more impact cost-benefit.

Introduction and Radiology staffs are exposed to low doses of the occupational ionizing radiation. The long term intermittent exposure to low radiation doses may damage cells and result in various adverse biological effects. The blood cells have been found to be the most radiosensitive cells to ionizing radiation. The aim of this study was to investigate the relationship between low dose of occupational exposure to ionizing radiations and hematological parameters, including red blood cells, white blood cells and platelet counts of the radiology staffs of Yasuj hospitals.
In this case-control study, 17 radiology staffs (8 females and 9 males) working in Yasuj hospitals and 17 healthy non-radiation workers were participated. Occupational radiation histories were determined by film badge dosimeter recordings for each radiology staffs. Hematological parameters of the participated in this study were recorded by means of cell counter sysmix machine. Statistical analysis was performed by SPSS 21. The statistical analyses were done by one way analysis of variance (ANOVA), and Pearson’s correlation coefficient.
The values of total white blood cells and platelet count were significantly lower among radiology staffs compared to control but no significant difference was found between their red blood cells. No significant correlation was observed between gender, and work experience with blood parameters. The file badge records for all of the radiology staffs were under occupational dose limits.
Platelet and white blood cells parameters are found to be sensitive to low dose occupational ionizing radiation for radiology staffs. We will suggest that the radiology staffs should use their personal dosimeters while their hematopoietic system should be evaluated by blood cells count.