نشریه ارمغان دانش
سال نوزدهم شماره 8 (پیاپی 91، آبان 1393)

Vitamin BI (thiamine) plays an important role as a coenzyme in energy metabolism and in the maintenance of nerve cell function. The purpose of this study was to investigate the effect of short – term supplementation of thiamin pyrophosphate on the aerobic and anaerobic power in girl soccer players. The study was carried out as a double – blind cross-over clinical test. The statistical society of this research included young girl football players of Neiriz city، who regularly took part in sport activities and training. Among them، 16 people were randomly chosen to participate as samples in the execution of this project. For the initial evaluation of variables under study، two days prior to the supplementation period، the subjects، before being put in the supplemented and placebo groups underwent Bruce and Rest test. At first the Rest test was carried of and the Bruce test followed two hours later. After being assigned to supplement and placebo groups، the subjects took thiamine and placebo for a week. Twice daily، they received 100 mg. Of thiamine supplementation or placebo (at 8 am. And 4 pm.). In order to study the changes and analysis of the data، the one – way variance analysis test with repeated measurement was applied. A significant level was set of less than 0/05. A significant increase in the amount of maximal oxygen intake (47/65 ± 2/70) and the travelled distance and time till (16/21 ± 0/68) the end of the activity was observed in the supplemented group (P<0/016). Mean peak aerobic power، anaerobic power and fatigue index group (s) to supplement the 75/02 ± 490/95، 86/41 ± 385/48، 12/05 ± 29/64 respectively and compared to the between the peak and the average anaerobic power، fatigue index and between the groups. Thiamine supplementation، Could lead to further decomposition of pyruvate، Aerobic activity increases cellular metabolism and the Krebs cycle routes، Resulting in increased oxygen consumption is.

Urinary tract infections (UTIs) are the most common medical complication experienced by individuals living with SCI. Several factors are responsible for the high prevalence of UTIs in individual with SCI. Concerns regarding the overuse of antibiotics in individuals with SCI and emerge multi-drug-resistant bacteria, has prompted consideration for consumer –directed alternatives to improve urinary tract health. This study was designed to evaluation of cranberry juice on bacteriuria and pyuria and in spinal cord injured patients with neurogenic bladder in Shahrekord, Iran. This study was randomized, double-blind, placebo-controlled trial. 60 patients (51 male and 9 female) with creatinine levels below 1. 5 mg/dl and in the analysis of their urine white blood cell (WBC) counts were greater than 10 in a high-powered field (pyuria) or with a presence of bacteriuria (>= 104 cc/ml) in their urine culture selected in this study. Urine analysis and culture were carried out at before and after intervention. Samples was divided into two two groups of 30. The case patients were given a dose of 250 to 300 ml of cranberry juice cocktail with 30% concentration, daily with meals. The control group was fed the same amount of a placebo cocktail. After two weeks, first morning urine analysis and culture test were done. Data collected and analyzed using K-squared method using the SPSS software and Paired-T test technique. Urine analysis and culture before and after interventions show, Urinary PH in case and control groups did not any significant statistical difference before and after intervention (P>0. 05). A change in pyuria and bacteriuria levels in case patients was observed after the treatment which was statistically significant (P<0. 05). However, after treatment, the bacterial colony count in the case patients had dropped as a result of the cranberry juice consumption. the urine foul-smell was noticeably reduced in treated case patients (CI>95٪). Consumption of cranberries can be effective in treating SCI patients with UTI under certain conditions. The effectiveness was most profound in patients with normal GFR who did not use urinary catheter for urination. The effectiveness was especially seen in short term reduction of pyuria and bacteriuria levels and in reduction of foul-smelling of patient's urine.

introdaction & Magnesium playes an important role in the structure and cell metabolism. In this research, anti oxidant effects of magnesium sulfate against oxidative stress effects of was investigated. In this experimewtal study 36 male adults rats were placed into4 groups, with nine rats in each group. Treatment was then carried out as follows. Group I received olive oil (intraperitoneally) and distilled water (intragastrically), and served as the untreated control animal group. Group II was the hepatotoxicity group that was given a suspension of (i.p., 0.5 mL/kg b.wt, 50% in olive oil), twice a week. Groups III received dissolved in distilled water daily via an intragastric tube(.0.15M g/kg b.wt.)Groups IV were the treatment group that received dissolved in distilled water daily, via an intragastric tube 0.15 g/kg b.wt.),with (i.p., 0.5 mL/kg b.wt., 50% in olive oil) twice a week. After a 28-day treatment period, the animals were deprived of food overnight, anesthetized by exposure to diethyl ether, and then sacrificed by decapitation. Blood ws collected from the jugular vein, and serum was separated and used for liver marker assays. Levels of ALT, AST ALP and GGT were estimated using commercial kits. The liver homogenates were used for the assay of SOD. All data were expressed as means S.E.M. Statistical analysis was carried out using one-way ANOVA followed by a Tukey post hoc test. The criterion for statistical significance was p<0.05. In the -treated control group, serum ALT, AST, ALP and GGT were significantly increased as compared with the untreated control group. In contrast, the group that also received mgso4 Showed signifi cantlyless elevated levels of ALT, AST, ALP and GGT. compared to normal levels. Liver SOD activity in -treated rats was decreased significantly when compared with the the control group. Treatment with protected this enzyme activity. The results of this study showed that magnesium sulfate has improved oxidative effect of in the liver rats because of its antioxidant properties.

Studies show that amphetamines can influence the hypothalamic pituitarythyroid axis. The aim of this study was to investigate the effects of Shisheh (met-amphetamine) on serum levels of T3, T4 and TSH in male rats. In this laboratory experimental study 25 male Wistar rats were randomly divided to control, saline receiving, 2mg/kg, 4mg/kg, and 6mg/kg of Shisheh receiving groups of 5 rats in each. The injection was carried out intraperitoneally once a week for 6 weeks. After serum collection, T3, T4 and TSH levels were measured using ELFA method and the data were statically analyzed using ANOVA. Serum level of T3 and T4 was not significantly changed in rats receiving 2mg/kg of Shisheh compared with control animals. Serum level of T3 and T4 was signifintly increased in animals receiving 4mg/kg and 6mg/kg dose of Shisheh compared with the control rats (P<0.05, P<0.001, respectively). Serum TSH level was significantly increased in rats receiving mg/kg, 4mg/kg, and 6mg/kg of Shisheh compared with control animals (P<0.001). Our results indicate that Shisheh injection can enhance thyroid gland activity via acting on pituitary-thyroid axis, resulting in increased serum T3, T4 and TSH.

Because of emerging multi-drug resistance (MDR) Pseudomonas aeruginosa strains, treatment of burn patients infected by this bacterium is difficult. The aim of this study was to detect antimicrobial profile and molecular epidemiology of metallo-beta-lactamase (MBL) producer strains. In this cross-sectional investigation 270 Pseudomonas aeruginosa isolates were collected from the burn patients. Carbapenem sresistance strains were detected by phenotypic E-test method. Susceptibility profiles of metallo-β-lactamase (MβL) enzyme producing isolates of this bacterium to 11 antimicrobial drug were determined by disc diffusion method according Clinical and Laboratory Standards Institute (CLSI) guidelines. The genetic correlations between isolates were determined by Pulsed-Field Gel Electrophoresis (PFGE) method. Among 270 P. aeruginosa isolates, 60 (22.2%) strains showed resistant to meropenem (MEM) and imipenem (IMI) and were considered as metallo-β-lactamase positive. All metallo-β-lactamase positive isolates were resistant to five tested antimcrobial while their sensitivities to the three best effective antibiotics including ciprofloxacin, amikacin and ceftazidime were 1.7%, 6.7 % and 23.3%, respectively. Majority of the isolates (71.6%) showed more than 80% similarity based on the drawn dendrogram. Our results showed, the tested antimicrobials are not safe to prescribe for burn patients. According PFGE pulsotypes, a limited number of P.aeruginosa types are common in the hospital burn unit which infect the patients hospitalized in this ward.

Introduction & With respect to the outbreak probability of dangerous infections among the patients, the water sources of dentistry units were taken into consideration, in view of microbial contamination. The objective of this study was to assess of pathogenic organisms of the water used in the dentistry units of Yasuj city. In this research 120 samples of water from poir and turbine of units (before and after flushing) and two samples of urban water were collected. Sampling was performed on the first weekday (48 hours after the units were switched off) and mid week(16 hours after the units were switched off) before starting work. The samples were cultured on EMB Agar and Blood Agar and incubated at 37oC for purification for Gram positive or Gram negative bacteria and it was identifying with biochemical diagnostic test. Also the samples were counted by standard plate count. The average count of bacteria before flushing in Poir on the first weekday was 5360CFU/L and turbine was 2800 CFU/L and count of bacteria after flushing in Poir on the first weekday was 1040 CFU/L and turbine was 1020 CFU/L. While this result for midweek day: The average count of bacteria before flushing in Poir was 3220 CFU/L and turbine was 2720CFU/L and count of bacteria after flushing in Poir was 1772 CFU/L and turbine was 980 CFU/L. Several samples of before and after flushing were contaminated with E.coli, Pseudomonas, Proteus, Kelebsiella, gram positive bacilli, Streptococci, Staphylococci, Diplo cocci and Yeast. According to the result of this study the contamination rate of the unit’s water was high and it’s rate reduced after flushing. The patients saliva causes water unit contamination and it constitutes biofilm in pipe of unit. Existence of E.coli shows the contamination of water to sewage and staphylococcus explains contamination due to return of the patient’s saliva into suctions. dental units waterlines showed bacterial contamination which was eliminated after flushing.

Vaspin hormone is new Adipokine from adipose tissue whose metabolic changes can have a significant effect on adipose tissues, muscles, liver and inflammation. It is also associated. The aim of the present study is to investigate the effect of two kinds of anaerobic and aerobic exercise programs on Vaspin Level Plasma hormone changes in female Sprague Dawley rats. This is experimental study 55 rats were randomly divided into two experimental (aerobic and anaerobic) and pre-test groups. Based on the training program, the experimental group rats did aerobic and anaerobic exercises for eight weeks. 15 pre-test blood samples were taken before the exercise and 40 blood samples were taken from the experimental group (20 aerobic and 20 anaerobic rats) to measure after the exercises. paired t-test and independent t-test coefficient were used for data analysis. The results showed that there was a significant difference in vaspin levels after eight weeks of aerobic and anaerobic exercises (p=0/0001). However, no significant difference was observed between the amount of vaspin plasma levels in the aerobic and anaerobic groups (p=0/47). Considering the reduction of Vaspin hormone after both aerobic and anaerobic exercise programs, it seems that vaspin level changes are not affected by physical activities. Both types of exercise programs (aerobic and anaerobic) can be recommended as a major deterrent for various diseases due to the reduction of adipose tissues after regular exercise and the importance of vaspin reduction in the prevention of diseases related to metabolic disorders.

EnteroinvasiveEscherichia coliis one of the important agents of invasion to intestinal epithelial cells, damage and cell death which due to dysentery. The aim of this study wastoDetection of EnteroinvasiveEscherichia coli by PCR technique from Children’s Diarrheain yasuj. This cross-sectional study was performed on 200 stool samples taken from children with diarrhea in Yasuj. After initial identification of E.coli strains by culture and biochemical tests, EIEC gene such as ipaH detected by PCR technicque and antibiotic susceptibility of isolates was evaluated by using disc diffusion (CLSI) method. Out of all examined samples, 16(8%) EIEC were separated. Antibiotic susceptibility test showed that the most susceptible antibiotic is ciprofloxacin for EIEC and also most resistant antibiotic is ceftizoxime. Results showed that EIEC strains have a moderate prevalence than other studies in our study area. Therefore, for importance of this strain to producing dysentery, hospital-wide surveillance using molecular techniques hase been proposed in other regions of country.