Journal of Sciences, Islamic Republic of Iran
Volume:25 Issue: 4, Autumn 2014

Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we proposed a new and very simple protocol using uncoated fused silica capillary for CE of cffDNA purified from serum of pregnant women. The proposed protocol was able to determine the presence of small fetal DNA fragments and it also confirmed the optimization of sonication performed on purified cffDNA samples.

The genetic diversity of nine wild Secale strictum accessions was studied using seed storage protein profiles and phenotypic traits. Offsprings and phenotypically superior offsprings of the nine wild accessions were also evaluated and compared with their parental accessions to assess their genetic variability based on seed storage proteins and seed germination parameters. High genetic variation was observed for both seed storage protein profiles and phenotypic traits. The protein banding data were investigated in relation to phenotypic traits and indicated no influence of polymorphic bands on quantitative traits. Seeds of superior offsprings showed less genetic variability than both wild and offsprings of S. strictum accessions suggesting that more intensive breeding practices may have resulted in a further erosion of genetic variability. Neighbor-joining cluster analysis showed that wild populations, offspring and phenotypically superior offspring accessions were separated into three groups. This suggests that founder effects and subsequent selection have had more effect on the genetic differentiation among these accessions than geographical separation. The results demonstrated that the study of genetic diversity and differentiation between the parents and their offspring using seed storage protein profiles provides important information for the breeding and conservation of S. strictum germplasm.

An improved, simple, and facile synthesis of 3,4-dihydropyrimidin-2(1H)-ones by employing three-component, one-pot condensation reaction of β-keto ester, aromatic aldehydes, and urea or N-methylurea using LaCl3/ClCH2COOH as an inexpensive and green chemistry catalyst system under solvent-free conditions described. Compared with the classicalBiginelli reaction conditions, this method has the advantages of good to excellent yields (80-99%) and short reaction time.

A mild and green method has been developed for the preparation of coumarins derivatives through the one-pot reactions. In our study,various phenols and ethyl acetoacetate compound in which we used silica-supported boron sulfonic acid [SiO2/B(SO4H)3] as a new, highly efficient and tri-functional inorganic Brønsted acid catalyst were processed under solvent-free condition and thermal. The catalysts were separated easily from reaction mixture, regenerated after a simple activation step and reused at least six times without significant loss in catalytic activity. The most significant features of this procedure are:short period of conversion and excellent yields, simple experimental procedure, environmentally benign, the elimination of corrosive liquid acids and green aspects by avoiding toxic conventional catalysts and solvents.

Cobalt oxide (Co3O4) nanoparticleshave been synthesized by hydrothermal method using mixture of cobalt(II)chloride, Triton X-100 and KOH in an autoclave at 180 °C for 6 h followed by heating at 400 ºC for 3 h in air. The product have been characterized by Fourier transform infrared (FT-IR), UV-Vis spectroscopy, powder X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Optical properties of the Co3O4nanoparticleshave revealed the presence of two band gaps, viz.2.9 and 2.4 eV. Data fromvibrating sample magnetometer (VSM) confirm the purity of the product along with single phase paramagnetic behavior.

The presence of radon in drinking water causes health risks associated with exposure from both inhalation and ingestion. Since the studies show that faults near the water resources have a critical effect on the concentration of radon, Kouhbanan active fault zone, to find water resources with high radon concentration was chosen for the following investigation. Water samples were taken from all of the 39 drinking water springs and Qanats in the Kouhbanan region, both in the summer and winter. Its length is 280Km and is aligned from Bahabad city in north-west to the Kerman city in south-east. Some evidence of the activity of this fault system are young morphotectonic structures and earthquake events along of this faults. To determine the content of sample’s radon, radon detector (RAD7) was used. In six out of water samples, radon levels was more than proposed emission 11.1kbm-3(MCL) by Environmental Protection Agency (EPA). The average annual effective dose in 2 regions is more than reference level of the European Union Council and world health organization (0. 1mSva-1) according to the average effective dose for ingestion of radon in water (0.002mSva-1) and inhalation (0.025mSva-1) by United Nations Scientific Committee on the Effective of Atomic Radiations. This study has concluded that the mean effective dose for ingestion and inhalation of radon in Kouhbanan fault zone, is greater than the amount recommended for water samples in 6 region.

A total 7 species and 13 genera of marine gastropod assemblages are reported for the first time from the Varehh Zard section (Asmari Formation), north of Pole Dokhtar, Lorestan Basin. The section under study, the Asmari Formation, lies between the Shahbazam Formation at the base and Gachsaran Formation at the top. According to the distribution of index foraminifera, the Asmari Formation is Early Miocene (Aquitanian-Burdigalian) in age. In general, gastropod assemblages have been observed in the upper part of Asmari Formation (Burdigalian). The Miocene and even Oligocene gastropod faunas, relatively similar to the Vareh Zard section, reported from Tethys and Proto- Indo- Pacific Ocean, indicate that a passage was open during this interval. In this study, Oostrombus auricularius, Anazola elavula, Conus diversiformis, Turbo thouvignoni, Campanile pseudoobeliscus, Cassis mamilliaris, Psudophasianus elatus, Paroxystele, Ampullina sp., Architectonica cf. carocollata are described with special emphasis on their distribution in the Tethys and adjacent bioprovinces. Lyra sp., Cerithium rude and Ampullospira sp. are restricted to the Proto- Indo- Pacific Ocean.

In (Golchin A. and Rezaei P., Subpullbacks and flatness properties of S-posets. Comm. Algebra. 37: 1995-2007 (2009)) study was initiated of flatness properties of right -posets over a pomonoid that can be described by surjectivity of corresponding to certain (sub)pullback diagrams and new properties such as and were discovered. In this article first of all we describe po-flatness properties of -posets over pomonoids by po-surjectivity of corresponding to certain subpullback diagrams. Then we introduce three new Conditions, and and investigate the relation between them and Conditions, ,, , and. Also we describe these properties by po-surjectivity of corresponding to certain subpullback diag In (Golchin A. and Rezaei P., Subpullbacks and flatness properties of S-posets. Comm. Algebra. 37: 1995-2007 (2009)) study was initiated of flatness properties of right -posets over a pomonoid that can be described by surjectivity of corresponding to certain (sub)pullback diagrams and new properties such as and were discovered. In this article first of all we describe po-flatness properties of -posets over pomonoids by po-surjectivity of corresponding to certain subpullback diagrams. Then we introduce three new Conditions, and and investigate the relation between them and Conditions, ,, , and. Also we describe these properties by po-surjectivity of corresponding to certain subpullback diagrams and describe Conditions, and by weak po-surjectivity of corresponding to certain subpullback diagrams. rams and describe Conditions, and by weak po-surjectivity of corresponding to certain subpullback diagrams.

Let g be a fixed element of a finite group G. We introduce the g-noncommuting graph of G whose vertex set is whole elements of the group G and two vertices x,y are adjacent whenever [x,y] g and [y,x] g. We denote this graph by. In this paper, we present some graph theoretical properties of g-noncommuting graph. Specially, we investigate about its planarity and regularity, its clique number and dominating number. We prove that if G, H are isoclinic groups with |Z (G)|=|Z (H)|, then their associated graphs are isomorphic.