مجله دانشگاه علوم پزشکی بابل
سال بیستم شماره 10 (پیاپی 133، مهر1397)

Hematopoietic stem cells are one of the new therapeutic options for treating skin ulcers. Recently, a new perspective has been developed to better utilize stem cells using nanofiber scaffolds. The present study was conducted to investigate the effect of polycaprolactone/gelatin (PCL/GT) nanofiber scaffold on the therapeutic function of hematopoietic stem cells. In this experimental study, 15 male BALB / c mice were divided into three groups of five, including the control group, the group receiving stem cells in the wound site (cell group) and the group receiving cell + PCL nanofiber scaffold. PCL/GT nanofiber scaffold was prepared by electrotherapy. After hematoxylin and eosin staining, the parameters of epidermal repair and hair follicle formation in the wound site were evaluated by fluorescence microscope and Image J and SPSS programs.
FINDINGS: On day 28 after transplantation, the highest and lowest epidermal thicknesses were observed in the cell + scaffold group and control group, which were 10.5±0.3 and 27.3±0.9 μm, respectively, which was significant (p<0.05). Moreover, the highest and lowest number of hair follicles were observed in the cell + scaffold group and control group, respectively; 5.2±0.2 and 4.2±0.3. The difference between the two groups was significant (p<0.01) Polycaprolactone / gelatin (PCL/GT) nanofiber scaffold significantly increases the therapeutic function of hematopoietic stem cells in the wound site

Various drugs such as propofol or midazolam with or without fentanyl are used for colonoscopy. In this study, we compared the effect of sedative and the recovery time of dexmedetomidine and fentanyl during elective colonoscopy. In a double blind clinical trial, 80 patients (20-70 years) with colonoscopy were randomly divided into two equal groups. In the intervention group, dexmedetomidine was administered 1mcg / kg and in the control group, fentanyl 0.5 mg / kg before the start of colonoscopy. Propofol (20 mg) was administered as a bolus dose if needed during colonoscopy.
The sedation score based on the Ramsay sedation scale and the mean bolus dose of propofol was recorded. Recovery time and pain after colonoscopy based on Visual Analog Scale (VAS) was recorded before the discharge. The two groups did not have a significant difference in age, sex and sedation score (P> 0.05). The mean bolus dose of propofol in the fentanyl group was 72.00 ± 14.00 mg and 7.7 ± 0.24 mg in the dexmedetomidine group (P = 0.000). The recovery time in the fentanyl group was 4.38 ± 2.28 minutes and in the dexmedetomidine group was 2.63 ± 2.21 minutes (P = 0.000). Pain score after colonoscopy (VAS) in the fentanyl group was 2.30 ± 0.99 and in the dexmedetomidine group was 1.98 ± 0.70 (P = 0.039). The combination of dexmedetomidine and propofol is a more appropriate drug than fentanyl and propofol for colonoscopy due to shorter recovery

Deprivation of maternal care as a stressor causes disruption in cognitive and neurochemical activities of the brain. The aim of this study was to evaluation the effect of deprivation of maternal care on LTP induction in neurons of hippocampal CA1 region in morphine-dependent rats. This experimental study was conducted among 40 45-day-old male rats in control group, morphine-dependent group, and 3 groups of rats that were deprived of treatment for one, two and three weeks after birth for 3 hours daily. Except for the control group, the rest of the groups received 10 mg/kg body weight morphine sulfate subcutaneously every 12 hours for 10 days. On the eleventh day, the symptoms of deprivation syndrome were investigated by the Gellert-Holtzman method, and on the following day, the synaptic plasticity of neurons in CA1 region was studied.
FINDINGS: The Gellert-Holtzman score in the morphine-dependent group was 14.98±4.16 and increased to 31.79±5.12 in the group that was deprived of maternal treatment for 3 weeks (p<0.001). Although morphine dependence did not affect basic responses of CA1 region neurons and LTP induction, deprivation of maternal care reduced the range of basic responses from 1.01±0.04 in the morphine-dependent group to 0.68±0.09 mV in the group that was deprived of maternal care for 3 weeks (p<0.001) and prevented LTP induction (p<0.001) in a time-dependent manner. The results of the study showed that deprivation of maternal care undermines the postsynaptic potential of the hippocampal CA1 region following morphine administration and disrupts the synaptic plasticity of the neurons in this region

Green production of nanoparticles is an environmentally friendly method in which natural solvents are used. Green nanoparticle synthesis methods have priority over chemical and physical methods due to the reduction of cost, time and energy. The purpose of this study was to biosynthesize zinc oxide nanoparticles and evaluate the antibacterial activity of these nanoparticles. In this experimental study, the extract of Eucalyptus melliodora was combined at a ratio of 1: 1 with 0. 1 M zinc sulfate and kept at room temperature for 15 minutes. Synthesis of zinc oxide nanoparticles was confirmed by spectrophotometric methods, mean diameter of nanoparticles meaturement, X-ray diffraction and electron microscopy (SEM). Then, the MIC of zinc oxide nanoparticles was measured on standard strains of Staphylococcus aureus 1431PTCC, Bacillus cereus 1015PTCC, Escherichia coli 1399PTCC and Pseudomonas aeruginosa 1571PTCC.
FINDINGS: The absorption peak of ZnO NPs was observed at of 350 nm. The SEM showed ZnO nanoparticles was spherical shape of size ranging from 30 to 50 nm. The highest MIC was observed for Pseudomonas aeruginosa with 0. 0019 mg / ml and the lowest MIC for Bacillus cereus with 0. 62 mg / ml. Also, in comparison with ZnO nanoparticles, Eucalyptus extract and Zinc sulfate had the most antimicrobial activity for zinc oxide nanoparticles and their MIC had significant difference in the effect on the bacteria tested (p <0. 05). The results of this study showed biological nanoparticles of ZnO and aqueous extract of Eucalyptus as well as zinc sulfate have antimicrobial properties, but the most antimicrobial effects were observed by ZnO NPs

Nowadays, in order to improve the health and reduce the risks of chemical preservatives in the food industry, researchers have focused on the use of natural compounds, especially plants essential oils that have antioxidant and antimicrobial properties, due to the increased risk of bacteria Escherichia coli and Listeria monocytogenes in foods, in this study the antimicrobial and antioxidant properties of Origanum vulgar ssp. Gracile essential oil were investigated in vitro. In this experimental study, Origanum vulgar ssp. Gracile plant was prepared from Kurdistan and after drying and extraction of essential oils, components were identified using GC-MS device. Strains were prepared from the bacteriology laboratory of the Faculty of Veterinary Medicine, Tabriz University. In order to determine the minimum inhibitory and fatality concentration, various concentrations of extract were used (0.312, 0.625, 1.25, 2.5, 5 and 10 %), the minimum inhibitory and fatality concentration were determined using microdilution method. The antioxidant activity was performed by using the DPPH method and Total phenolic material was measured by Folin Ciocalteu reagent.
FINDING: Based on the results of GC-MS analysis, carvacrol (49.33%) had the most component of the extract composition. The MIC and MBC of essential oils on Escherichia coli were 1.25 and 2.5%, respectively, and 0.625 and 1.25% for Listeria monocytogenes, respectively. In addition, the antioxidant activity 12.45±0.03 μg/ml, and the content of phenol 39.13±4.13 mg of gallic acid in grams of essence was calculated. The results of this study showed that Origanum vulgar ssp. Gracile essential oil had antioxidant properties, and the essence had an inhibitory effect on both bacteria and Listeria monocytogenesis was less resistant than Escherichia coli to the essential oil

The skin squamous cell carcinoma (CSCC) is the second most common skin cancer. The correlation between severe tissue eosinophilia with optimal and undesirable prognosis, or even an ineffectiveness effect was related. The aim of the present study was to investigate the correlation between tissue eosinophilia and prognosis in patients with head and neck squamous cell carcinoma.

In this cross-sectional study, 33 patients with squamous cell carcinoma of the pathology department of Shahid Beheshti hospital of University of Babol University of Medical Sciences (age, sex, tumor site) were collected and histopathologic in the vascular and nervous invasions was studied. Numbers of eosinophils (eos.) were counted in 15 successive fields at invasive fronts of HNCSCCs (area of 15 fields=1.2mm2) at×400 magnification with optical microscope. The correlation between the mean number of eosinophils per Mm² and the severity of eosinophilia (mild and severe) and clinical-histopathologic factors was analyzed.
FINDINGS: Mean eos/mm2 was 99.1913±104.39897. Overall survival rate, disease-specific survival rate and disease-free survival rate were %72.72, %61.53% and %84.84, respectively. There was inverse significant correlation between disease-free survival with eos/mm2 (p=0.043). There were no significant correlation between other clinical-histopathologic factors with mean eos/ mm2.There were inverse significant correlations between 5-year overall survival rate and 5-year disease-free survival rate with severity of tissue eosinophilia (p=0.02 and 0.013 respectively).There were not significant correlations between 5-year disease-specific survival and other factors with severity of tissue eosinophilia.

There was inverse correlation between severity of tissue eosinophilia with survival

wear is the one of factors which can influence on the clinical performance of composites. Tooth bleaching agents can effect on these materials. The aim of this study was the comparison of the effect of different bleaching regimes of carbamide peroxide on wear of Z250 composite. in this in vitro study, 40 disk shape sample of Z250 were made with 7mm in diameter and 2mm in thickness by plastic molds. Samples divided in 4 groups randomly (n=10): In first group (control group), samples were toothbrusted (for 20000 cycles) with no carbamide peroxide (were kept in 37 °C distilled water for two weeks), in second group, samples that were tooth brusted with 10% carbamide peroxide (4 hours daily for 2 weeks). In third group samples were toothbrusted with 16% carbamide peroxide (3 hours daily for 2 weeks). Fourth group were toothbrusted with 22% carbamide peroxide (1 hour daily for 2 weeks) Weight of samples were recorded before and after wear test. The mean of weight loss (gr) of samples in control group was 0.001±0.0016, for second group was 0.0048±0.0089, for third group was 0.0013±0.0025 and for fourth group was 0.0005±0.0002. There were decrease in samples wear with increasing of peroxide concentration but this difference was not statistically significant. Based on the findings of this study, carbamide peroxide 10%,16% and % had no effect on tooth brush wear rate of Z250 composite.

Chrysin is a naturally, bioactive compound which is extracted from plants, honey and propolis. Chrysin has pharmacological activity, including antioxidant, anti-inflammatory and anticancer properties. This study was performed to compare the effect of Chrysin and its nanoparticles on MCF7 cell line. In this study, chrysin nanoparticles were prepared using chitosan, and the nanoparticles prepared dissolved in water and cytotoxic effects of concentrations of 20, 40 and 80 μM / ml and chrysin dissolved in DMSO at the same concentrations were investigated on MCF7 cells. Cell viability was measured using MTT assay after 24, 48 and 72 hours of treatment and compared with the viability of the control samples.
FINDINGS: Chrysin and Chrysin nanoparticle prevented the growth and proliferation of MCF7 cell line breast cancer. These effects depend on the concentration of chrysin and its nanoparticles and it has been shown that the nano-particles of chrysin at a concentration of 40 μM in 72 hours after effect on the cells are significant with p <0.001, while at the same concentration in 48 And 24 hours later with a significant p <0.01. The IC50 is approximately 40 μm. Chrysin nanoparticles exhibit antiproliferation activity on breast cancer cells in vitro, and its effect is greater than chrysin. The results of the experiment showed that it is statistically significant at 40 μM compared to chrysin

Candida albicans infection is a problem of growing clinical importance, particularly in immunocompromised populations. This study aimed to investigate the inhibitory effect of carvacrol on the hyphae formation and expression of C. albicans HWP1. This cross-sectional study was done over a 6-month period in 2016-2017. Vaginal, mouth and skin surface swabs were obtained from immunocompromised patients. Colonizing clinical isolates of C. albicans were identified and drug susceptible isolates detected using WHONET software. The susceptibility test for carvacrol (range 6.25–300 μg/ml) were carried out with a broth microdilution according to the CLSI guidelines against drug susceptible C. albicans. The time kill assay of carvacrol (range 2 × MIC to ¼ × MIC) was determined. Hyphae inhibition was evaluated by light microscopy. We determined the expression levels of HWP1 implicated in hyphae formation of C. albicans ATCC 14053 cells by quantitative RT-PCR.
FINDINGS: Ten colonizing clinical isolates of drug susceptible C. albicans were identified. Carvacrol was inhibited the growth of all drugs susceptible isolates of C. albicans (MIC range, 25-200 µg/ml). Time kill curve assay demonstrated that carvacrol could significantly inhibit the growth of C. albicans (p≤0.05). Carvacrol efficiently prevented hyphae formation in drug susceptible C. albicans. The expression levels of HWP1 gene were down-regulated by 1.82- and 1.62-fold at concentrations of 2 × MIC and 1 × MIC of carvacrol, respectively. These results suggest that carvacrol could provide an improved and safe clinical approach in treating Candida infections by prevention of hyphae formation

Tabarestan (current Mazandaran) is considered one of the scientific and medical centers in the world of science and the Islamic world in northern Iran and the south of the Caspian Sea. The emergence of scholars in this area shows the centrality of science in this amazing geography. In this research, we investigate the life, scientific experiences and works of Abolhassan Ahmad bin Mohammad Tabari Taranji.