فصلنامه علوم پزشکی دانشگاه آزاد اسلامی
سال شانزدهم شماره 3 (پیاپی 45، پاییز 1385)

The study objective was to investigate time dependent effects of ischemic- reperfusion on cardiomyocyte apoptosis.

Male Sprague-Dawley rats (270-330 gr) were randomly divided into four groups (n=10) and anesthetized by sodium pentobarbital (50-60 mg/kg-IP). The hearts of rats in each of three treatment groups were removed and quickly mounted on a Langendorff apparatus and perfused by a modified Krebs-Henseleit solution under constant pressure at 37ºC. During the stabilization time, 30 minute regional ischemia (which was followed with 60, 90 or 120 minute reperfusion in respective T/60min, T/90 min and T/120 min reperfusion groups) was established, while in the control group the hearts were intact. Immunohistochemical detection of apoptotic cells was performed using an in-situ cell death detection (TUNEL) kit. The positive cardiomyocytes counted in five random high power fields in each sample. Data were presented as mean±SEM for each group.

In the control group, the number of apoptotic cells was 1.0±0.4, while in the treatment groups of T/60min, T/90 min and T/120 min reperfusion, the corresponding numbers were 2.0±0.5, 3.0±0.3 and 6.0±0.3, respectively. Although the difference between T/60 min & T/90 min and T/60 min & control group was not significant (p>0.05), the difference between T/60 min & T/120 min (p<0.001), T/90 min & T/120 min (p<0.01), T/90 min & control (p<0.01) and T/120 min & control (p<0.001) was significant.

We demonstrated that the duration of ischemic-reperfusion insult can affect the apoptosis changes in cardiomyocytes.

Formaldehyde is one of the chemicals which are widely used in different places. Considering the fact that the usage of this chemical can induce a reduction on the motility and viability of spermatozoa and/or make them abnormal, a precise study on testicular tissue structure, producing spermatozoa, seems to be necessary because no accurate study has previously been carried out on this issue.

In this experimental-quantitative investigation, a total number of 30 mice were divided into four experimental and one control groups. In this study, formaldehyde has been injected intraperitoneally at different doses for a period of time of 40 days. In order to provide the tissue specimens, the mice were initially anesthetized and after removing the testes and tissue preparing processes, the hematoxylin and eosin staining method was used and the sections of testicular tissue were studied under light microscope.

Comparison to the control group, disorganization in some seminiferous tubules, cellular irregularity and reduction in density of genital cells, increasing of intertubular spaces, and confusion of interstitial tissue, discoloration and vacuolization of leydig cell cytoplasm and also a thickness in basement membrane of spernatogenic epithelium were observed in the experimental groups.

The results of this study revealed that injective formaldehyde could induce some changes in testicular tissue and also it could increase the risk of infertility.