مجله دانشگاه علوم پزشکی سبزوار
سال بیست و چهارم شماره 2 (پیاپی 91، خرداد و تیر 1396)

Cyclotides are plant polypeptides characterized by their unique cyclic cysteine knot structural motif. They are present in many plants from the Violaceae, Rubiaceae and Cucurbitaceae families. But they also have variety of biological activities, including anti-HIV, antimicrobial and cytotoxic activities. Because of their exceptional stability, they have attracted interest as a potential starting point material for protein engineering and drug design.
The aim of this study is identification of genes encoding cyclotides from V. odorata, V. occulta, and V. ignobilis. We also study antimicrobial effect of cyclotides extracted from V. ignobilis. To reach this aim extraction of cyclotide was done by fractionation and solid phase extraction methods.
Findings indicate the presence of the cyclotide gene in all three plant species which studied. Examination of antimicrobial effect of partial purified cyclotide, defined S. aureus is the most susceptible bacterium among human pathogenic and X. oryzea is the most susceptible bacterium among all of studied bacteria.
In general, it seems that it can be used cyclotide peptide in most of the viola species because of their anti-peptide property in the pharmaceutical industry. Study of cyclotide sequences shows that despite of existence of conserved amino acids in the most of cyclotides, the differences in performance are due to sequence variation.

Clinical education is the most costly element of nursing programs. One of the importantbasics of nursing education comes into account. The clinical education course is important landmark in nursing education, the first professional capabilities of students to be established. This qualitative study was conducted in the emergency department .
This study is qualitative research with content analysis. The process of data collection consists of 20 semi-structured interviews and in-depth with the nurses in the emergency department (six nursing students and two nursing directors of wards, four nursing educators, eight nursing personnel) with notes field. By interview with actual experiences of nurses in clinical response, four open questions were collected. Data were analyzed with the using qualitative content analysis.
Data analysis resulted in the extraction of nine classes and three represent the experiences and perceptions of emergency roomnurses from clinical education and include the environment (Communications, safety environmental, environmental equipment), education (patient education, personnel training and training students) and performance of the director of sector (the role of management in the emergency department of education, the role of the matron of education, the role of the service provider's staff, communications, safety environmental, environmental equipment), education (patient education, personnel training and training students) and the performance of the sector (the role of management of the emergency department in education, the role and function of the mananger in education, the role and function of the service provider's staff).
Research findings based on the experiences of students, staff and educator showed some effective factors such as the patient's face to face training, to gain positive experience and some negative experiences such as lack of useable techniques that have been taught the students in the learning environment at the university like that quality in clinic, lack of equipment, and workload of personnel. Considering all the ideas, authorities will be able to identify the clinical education problems, plan appropriate training programs, and promote the quality of their clinical training.

Unstable condition of clinical situation of patients in Intensive Care Unit requires made nurses be competent in decision making. Despite the importance of clinical decision making in nursing, yet this is not the appropriate place in the profession. This study was conducted to evaluate the clinical decision making of nurses in Intensive Care Units of educational hospitals in Mashhad.
This descriptive study was conducted through stratified random on 233 nurses employed in educational hospitals of Mashhad University of Medical Sciences. PDAQ questionnaire was used to collect data. Data analysis was done using descriptive and inferential statistics (t -test, correlation and ANOVA) in SPSS V.16.
The results showed that nurses participation was in the average level at all phases of clinical decision making consist of identifying the problem (33.1±10.7), present of the solutions (34.1±10.3) and implement the solution (32.1±9.7). Nurses were acquired the highest score in the phase of present and evaluation of alternative solutions and lowest score in the phase of implement the solution.
According to results of this study, nurses’ participation was in the average level at all phases of clinical decision making. It seems that nursing education programs are not effective to enhance clinical decision making skills; therefore, the results of this study can be used to improve clinical decision making in nursing education and continuing education programs.

The economic feasibility of wastewater treatment plants requires applying some methods with ability to assess the cost-effectiveness of their systems. This study aimed to determine and compare the cost-effectiveness of two types of wastewater treatment systems which are used in Kermanshah province.
This is a descriptive-analytical study in which the information on the wastewater treatment plants were collected from water and wastewater companies and wastewater treatment plants operators, and the data were analyzed statistically using the SPSS software program. All costs, efficiency of treatment processes, and their cost-effectiveness index was calculated for existing systems and they were compared in terms of this ratio.
The results showed that, in activated sludge and natural systems, the average total efficiency, which is the result of mean removal of total suspended solids, biochemical oxygen demand and chemical oxygen demand, were respectively 76.37±18.19% and 61.69±19.02%. Also the results showed that the difference between the efficiencies of two systems was significant. The cost-effectiveness of activated sludge and natural systems were respectively obtained as 0.23 and 0.58.
According to the results, we can say that, despite the lower efficiency, natural systems are better than activated sludge systems and have better cost-effectiveness for wastewater treatment. These results indicates the natural systems is more successful in term of cost-effectiveness, and the most important factor in this success can be attributed to lower required investments and lower maintenance costs, compared to activated sludge systems.

An increasing trend in the use of ICT in the present time has caused some changes in the educational system and its methods so it has doubled the need for researches and studies to be done in this domain. So, this study was to compare three e-learning, Traditional-E learning and traditional learning in the pharmacology lesson of nursing student to move forward for changing the educational system to an effective one.
In this quasi-experimental study, 90 nursing students that have entered university during educational years of 2014 and 2015 and have passed the pharmacology lesson in their second semester were selected, based on purposive sampling methods, and agreed to participate in the study. They were then assigned to three groups that each had 30 students in it (e-learning, Traditional-E learning and traditional learning). By the end of examination they were compared on the mean results of their final tests and the impact of the three different methods were examined.
Findings: Based on the results of the mean final scores of the students at the end of test we found out that the difference between the three methods was significant. It was concluded that the Traditional-E learning method was successful for students due to an increase in their final results.
Traditional-E learning method can be used as an alternative in teaching pharmacology for nursing.

Printing industry with a high level of financial turnover is known as a main industry all around the world. Different hazardous factors, such as ergonomics, can be found in this industry. On the other hand, there are a variety of methods to assess ergonomic issues at the workplaces. Present study is aimed to analysis operational workers’ postures and to compare the results and concurrent validity of LUBA based on RULA results, in a printing and publication company in Qom, 2014.
This study considered all operational workers in a printing industry (n=80) in ten various jobs. Rapid upper limb assessment and postural loading on the upper body assessmenthave been applied to assess conditions. Gathered data was analyzed by Spearman and Kappa (P All workers were aged more than 36 years old and had 15-20 years’ work experience. Results of both methods depicted that most of postures were at the second action category and needed more studies. Correlation and agreements between postures were significant (P0.05).
Results illustrated acceptable correlation between different parts and reasonable agreement between wrists, elbow and shoulder’s postures, but neck’s posture and action categories agreements were not good. In addition, analyses illustrated that concurrent validity of LUBA is not acceptable in this industry. However, more researches are recommended.

Backgrounds and Antibiotics can’t be removedefficiently by conventional wastewater treatment.Nano Zero Valent Iron (nZVI) has high efficiency of degradation of contaminants such as heavy metal, nitro aromatic compounds and insecticides. Ozonation is one of the most well known methods for remove of chemichal-resistant materials like antibiotics of water and wastewater
This study is experimental. nZVI and ozonation were used to remove of penicillin G. for determination removal efficiency of penicillin, COD and TOC, factors: initial pH, dose of nano particle, initial penicillin dose, reaction time with nano particle and ozone were investigated.
Removal efficiency of penicillin G, COD, TOC in stage of reaction with nZVI were 96.35%, 73.13% and 47.45%, respectivly with reaction time: 60 min, initial penicillin concentration: 100 mg/L, COD: 1429 mg/L, TOC: 510 mg/L, pH=7, nano particle iron concentration: 0.25 g/L. In the stage of reaction with ozone, removal efficiency of penicillin G, COD and TOC were 99.5%, 96.35% and 92.65%, respectivly with reaction time: 13 min, pH=9, initial penicillin 20 mg/l, COD: 356 mg/L, TOC: 136 mg/L, volumetric flow rate of ozone: 5 L/min.
The best removal efficiency in stage of nano particles reaction was in pH=7, initial nZVI concentration 0.25 g/L, initial penicillin concentration 100 mg/L and time about 60 min. The best removal efficiency in stage of ozonation was in pH=7 and time about 9 min.

A 14 month old infant was referred to a vaseei Hospital from badkhoran village (a rural area of Sabzewar city) due to hematemesis and melena from 3 days ago. On arrival he was pale and ill looking with active bleeding from the mouth. The bleeding persisted in spite of active management with gastric lavage, intravenous omperazol, Vit K injection, FFP and blood transfusion. Physical examination and Emergency lab dates were negative for special etiologic findings. The patient re examined and a leech attached to the posterior pharyngeal wall was detected. The bleeding stopped soon after the removal of the leech and the patient was discharged from the hospital in good condition.