Zahedan Journal of Research in Medical Sciences
Volume:20 Issue: 5, May 2018

The present study compared attachment styles in patients with coronary artery disease (CAD) and healthy controls (non-CAD subjects).
This was a descriptive study with an ex-post facto design. The sample included 60 people (30 patients with CAD and 30 healthy controls), attending the office of an interventional cardiologist in Mashhad, selected by a convenience sampling method. They completed the adult attachment questionnaire. Data were analyzed using multivariate analysis of variance.
The results of multivariate analysis of variance revealed that there was a significant difference in attachment styles between CAD patients and healthy controls so that healthy controls in the secure attachment style and CAD patients in the insecure attachment styles had a higher score.
The findings confirm that attachment styles are more insecure in CAD patients than in healthy people. These results suggest that insecure attachment styles increase the risk of coronary heart disease.

Chemotherapy is currently used in treatment of different diseases, yet its various adverse effects has led to difficulties in its use for treating hydatid cysts. This leads to use of different non-chemical materials, such as plant extracts as alternatives to chemotherapy in order to cure hydatid cysts.
The aim of the present study was to investigate Quercus infectoria Olivier extract effects on hydatid cysts.
In this experimental study, various concentrations of Quercus infectoria Olivier extract at different exposure times were evaluated under laboratory conditions for their scolicidal effects on hydatid cysts. To this end, protoscoleces were collected from the livers of sheep affected by hydatid cysts and they were placed under exposure of various concentrations of Q. infectoria extract (10, 25, and 50 mg/mL) for various durations of 10, 20, 30, and 60 minutes. Staining with 0.1% eosin was used to specify the viability of protoscoleces. The SPSS software (version 19, Chicago) was the software used to perform the statistical analysis.
The obtained results indicate that Q. infectoria extract with the concentration of 50 mg/mL is able to kill all protoscoleces during 20 minutes. It is understood that the scolicidal effects of Q. infectoria on hydatid cysts was significant compared to the control groups (P The obtained results delineate higher scolicidal efficacy of Q. infectoria’s methanolic extracts; nevertheless, more research should be conducted to confirm the in vivo effects of Q. infectoria on curing hydatid cysts in human beings and different herbivorous animals.

Aflatoxins, a polyketide group derived from furanocoumarins, are highly toxic carcinogens produced by Aspergillus flavus and other closely related species.
In this research, the effects of Capparis spinosa, Withania somnifera, and Peganum harmala extracts on A. flavus growth and the expression of some major genes in aflatoxin biosynthetic pathway were investigated.
Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were measured and the expression of aflQ, aflO, aflS, and aflR genes was evaluated by using quantitative real-time polymerase chain reaction (qRT-PCR) technique.
Capparis spinosa extract had the lowest MIC value (200 mg/mL) and the extracts of W. somnifera and P. harmala had the highest MIC value (400 mg/mL). The extracts of W. somnifera and P. harmala had no fungicidal activity, and the C. spinosa extract had the lowest MFC value (400 mg/mL) and had the highest fungicidal effect on A. flavus. The expression of aflQ, aflO, aflS, and aflR genes was significantly decreased by the C. spinosa extract.
In general, the C. spinosa extract could have fungicidal and inhibitory effects on A. flavus and reduce expression of major genes in the aflatoxin biosynthetic pathway.

Milk forms a considerable part of the necessary materials needed for the physical and mental growth of all community members, especially children and the elderly. Since milk is a suitable culture medium for the growth of many bacteria, strict standards have been introduced for its safety. The aim of this study was to evaluate the coliform contamination of raw cow’s milk and the level of endotoxin in it.
Using random sampling, 43 samples of raw cow’s milk were collected in Isfahan, Iran. Coliforms were identified by biochemical tests. Then, the molecular method was employed for the definitive identification of the coliforms. Moreover, endotoxin levels were assessed employing the limulus amebocyte lysate (LAL) test.
Results indicated the presence of coliforms in 17 (39.45%) of the raw milk samples. Based on the molecular identification of the isolated coliforms, 10 samples were contaminated with Escherichia coli, five with Enterobacter spp., and two with Klebsiella pneumoniae. The results of the LAL test showed that 17 samples contained 0.250 units, six samples contained 0.125 units, and 20 samples contained 0.063 units of endotoxin.
There was a significant correlation between coliform contamination of raw cow’s milk and 0.250 unit/mL endotoxin (P

Human T-cell lymphotropic virus type 1 (HTLV-1) is a human retrovirus that causes two distinct diseases, adult T-cell leukemia (ATL) and a progressive and degenerative myelin disorder called myelopathy associated with HTLV-1 (tropical spastic paraparesis). Similar to human immunodeficiency virus (HIV), infections caused by HTLV-1 and -2 and retroviruses are persistent and long-lasting. Less than 5% of these infections are HTLV-related illnesses, but their treatment modalities are rare and their prognosis is poor. They are often fatal. Patients with β-thalassemia major are clearly at higher risk for HTLV-1 infection due to the need for frequent blood transfusions. The current study aimed at detecting the molecular screening for HTLV-1 infection based on fluorescent probe in patients with β-thalassemia. The current experimental study was conducted on 80 blood samples collected from patients with β-thalassemia major in Shahid Rajaee Hospital of Tonekabon, Iran. After DNA extraction by the Iranian kit, molecular biological system transfer (MBST) Cinna Gen company (Tehran, Iran), TaqMan real-time polymerase chain reaction (RT-PCR) was employed to detect DNA HTLV-1 genome and a tax gene-specific integrated with the genome of patients’ lymphocyte was obtained. Results of TaqMan RT-PCR indicated that out of 80 patients with β-thalassemia major referred to Shahid Rajai’e Hospital, two (2.5%) had HTLV-1. Compared to other molecular and serologic techniques, HTLV can be applied to detect HTLV-1 in blood banks due to its sensitivity, simplicity, higher speed, and the ability to simultaneously diagnose HTLV and lower costs than that of fluorescence molecular methods