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طب توليد مثل ايران
Iranian Journal of Reproductive Medicine
فصلنامه طب توليد مثل و باروري - ناباروري داراي رتبه علمی - پژوهشی به زبان انگليسي
سال پنجم، شماره 4 (پياپي 11)، Autumn 2007
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 | Human sperm DNA damage in the context of assisted reproductive technique
Youssry M ، Ozmen B ، Orief Y ، Zohni Kh ، Al، Hasani S Page 137 (انگليسي)
 Abstract  مشاهده متن [PDF 184KB] | | Fertilization involves direct interaction of the sperm and oocyte، fusion of the cell
membranes and union of male and female gamete genomes. The completion of this
process and subsequent embryo development depend in part on the inherent integrity of
the sperm DNA. Sperm genome quality has been emphasized for several years as
playing a major role in early embryogenesis. There is clinical evidence showing that
human sperm DNA damage may adversely affect reproductive outcomes and that
spermatozoa of infertile men possess substantially more DNA damage than do
spermatozoa of fertile men. Testing DNA integrity may help selecting spermatozoa with
intact DNA or with the least amount of DNA damage for use in assisted reproductive
techniques (ARTs). This review will focus on how sperm DNA is organized، what
causes sperm DNA damage and what impact this damage may have on reproductive
outcome.
کليدواژگان: Fertilization, Sperm DNA damage, Assisted reproductive technique (ART)
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| Non-invasive evaluation of embryo morphological plasticity by designing of new transgenic gene cassette
Rezaeian A, Kalantar SM, Farajnia S, Soleimani M, Baghi A, Aflatoonian A, Zeinali S Page 151 (انگليسي)
Abstract مشاهده متن [PDF 692KB] | | Background: Determination of transgenic embryos from non transgenic embryos sibling is an
important step in producing homozygous transgenic mice. These steps need by PCR or southern
blotting followed extraction of DNA، but both techniques require skill and consume time.
Objective: The aim of this study was simulation of high accuracy method using novel
enhanced green fluorescent protein (EGFP) gene cassette to eliminate some consume
time in livestock industry to assay high quality embryos and morphological plasticity.
Materials and Methods: We modified pQE-Tri systemic vector with EGFP and IRES
sequence to trace out coming planning of molecular farming transgene using coinjection
method.
Results: The combination of these sequences successfully showed the faint and normal
expression of transgene in mouse pre-implantation stage embryos. The low rate of
surviving green positive embryos as compare as only medium and physical treatments
could be partly from the physical damage caused by microinjection and gene integration.
Furthermore، application of the enhanced GFP marker facilitated subtle detection of
asymmetrical division appeared in some of transgenic embryos.
Conclusion: The results of current mouse simulation model imply that an efficient
production and propagation of transgenic livestock can be done by co-injection of every
economical gene with this novel transgene and also it can be suitable gene cassette for
numerous experiments and study of protein behaviors in living cells.
کليدواژگان: Enhanced green fluorescent protein (EGFP), Transgenic animals, Embryo
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| In vitro maturation media, cysteamine concentration and glutathione level affect blstocysts development in mouse
Mohammadi Roushandeh A, Pasbakhsh P, Alizadeh Z, Habibi Roudkenar M Page 159 (انگليسي)
Abstract مشاهده متن [PDF 115KB] | | Background: Preparation of oocytes is one of the critical factors that determine the
developmental competence of embryos produced by in vitro fertilization (IVF).
Objective: In this study، the effect of cysteamine، type of media and glutathione (GSH)
level on blastocysts development after in vitro maturation of mouse oocytes were
investigated.
Materials and Methods: Premature female mice were primed with pregnant mare
stimulating gonadotrophin (PMSG)، and germinal vesicle (GV) stage oocytes were
obtained 45 hr later. GV oocytes were cultured in presence of 0، 50، 100، 200 and 500
μm cysteamine in TCM199 and MEME media. After IVM، MII oocytes were in vitro
fertilized (IVF) and in vitro cultured (IVC) in order to observe embryo development. A
group of In Vivo Ovulated (IVO) oocytes after priming with PMSG and HCG also were
included in this study. 5،5-Dithio-bis (2nitrobenzoic acid) DTNB-recycling protocol
was used for GSH assay.
Results: Rate of IVM and IVF were improved in all oocytes treated with cysteamine in
the two medium except 500 μm (81% MII rate in TCM and 64% MII in MEME). Rate
of blastocyst in 100 μm cysteamine in TCM1199 and 200 μm in MEME was higher
compared to control groups (In TCM 45% and in MEME 35%). In vivo MII and GV
oocytes represented the highest and lowest GSH level respectively.
Conclusion: Our results revealed that the media and concentration of cysteamine can
affects on IVM، IVF and rate of blastocysts development on dose dependant manner.
کليدواژگان: Cysteamine, IVM, Blastocysts, GSH, Oocyte, Mouse.
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| Comparing the viability and in vitro maturation of cumulus-germinal vesicle break down (GVBD) oocyte complexes using two vitrification techniques in mice
Khosravi Farsani S, Mahmoudi R, Abdolvahhabi MA, Abbasi M , Sobhani AGh Page 165 (انگليسي)
Abstract مشاهده متن [PDF 150KB] | | Background: Vitrification is assumed to be a promising method to cryopreserve human
oocytes but still needs optimizing.
Objective: The aim of this study was to improve the single step and step-wise
vitrification effects on maturing mouse GVBD oocytes by ethylene glycol (EG) in
conventional straws.
Materials and Methods: Oocytes with compact cumulus cells were cultured for 3hr in
TCM199 supplemented with 10% fetal bovine serum (FBS) in 5% CO2 in air. GVBD
oocytes were randomly allocated into three groups. (1) Control (non-vitrified group)، (2)
exposed to single-step vitrification (contained of EG 20%+0.5M sucrose)، (3) exposed
to step-wise vitrification (2%، 5%، 10%، 20%EG +0.5M sucrose). In vitrification
groups،oocytes were thawed and underwent additional 21 hr maturation. Viability of
oocytes and maturation to MII stage were analyzed using inverted microscope and
additionally by staining of propidium iodide and Hoechst 33342.
Results: All non-vitrified oocytes were viable after 24 hr; however، viability of vitrified
samples in single-step group was significantly lower than that of the step-wise and
control Groups. Also، the maturation rate in the step-wise group was significantly higher
(p < 0.05) compared to single-step.
Conclusion: These results suggest that step-wise vitrification of GVBD oocytes as
compared to single step vitrification was better in the rate of survival and in vitro
maturation of oocytes.
کليدواژگان: Vitrification, Viability, In vitro maturation, GVBD, Oocyte, Mouse.
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| Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat
Talebi AR, Khalili MA, Nahangi H, Abbasi A, Anvari M Page 171 (انگليسي)
Abstract مشاهده متن [PDF 71KB] | | Background: Spinal cord injury (SCI) occurs most often to young men at the peak of
their reproductive health. Only 10% of SCI men can father children without medical
assistance due to potential impairments in ejaculation and sperm quality.
Objective: The main objective of this experimental study was to evaluate the
epididymal necrospermia- sperm death، after chronic SCI in rat.
Materials and Methods: Forty-five adult Wistar rats were divided into 3 groups of
SCI، sham، and control. Following laminectomy، SCI was induced onto exposed dura
matter (T10) of anesthetized rats. Sham group underwent laminectomy of T10 only;
while، control rats were not exposed to any type of injury or medication. The
spermatozoa from cauda epididymis were aspirated after 50 days for analysis of
necrospermia with three assays of Eosin-Y staining، Hypo-osmotic swelling (HOS)، and
Hoechst 33258 fluorescent dye.
Results: The rate of necrospermia in SCI rats was significantly increased when
compared with other groups (p<0.05). Also، the rates of necrspermia in SCI samples
were similar with application of 3 assays (Eosin-Y: 46.11±9.41; HOS: 45.88±8.89;
Hoechst: 46.76±9.31). Total necrospermia was not observed in any of the epididymal
samples.
Conclusion: The results showed that chronic SCI is associated with high rate of
epididymal necrospermia in mammals such as rats. It is، therefore، recommended that an
effective laboratory technique، such as Hoechst 33258 should be used for separation of
live and motile sperms from necrospermic ones for assisted reproduction program.
کليدواژگان: Spinal cord injury, Spermatozoa, Necrospermia, Rat.
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| Effects of Melatonin on histopathological changes after experimental ovarian torsion -detorsion in cat
Mostajeran F, Naderi M, Adibi SH Page 177 (انگليسي)
Abstract مشاهده متن [PDF 188KB] | | Background: During the detorsion of a torsioned ovary، oxidant agents are released and
melatonin as an antioxidant can reduce ischemia. We studied the histopathological
changes after using melatonin on experimental torsioned ovary in cat.
Objective: The aim of this experimental study was to investigate the effects of
Melatonin on histopathological changes in torsion – detorsion injury in cat ovary.
Materials and Methods: An adnexal torsion – detorsion model was created by using 20
adult cats randomly divided equally in to 2 groups of Saline and Melatonin. Ischemia was
induced by iathrogenic 360° clockwise torsion of the cat adnex for 3 hr. Reperfusion
was achieved for 3 hr. Melatonin or saline were injected intra peritoneally (10mg/kg) 30
min before ovarian detorsion in both groups. After 3 hr of ovarian detorsion، ovarian
tissue was removed and fixed in 10% formalin solution، embedded in paraffin and
evaluated for ischemic indices.
Results: Histological examination showed a significant improvement in ovarian
morphology in the melatonin treated cats. Edema and vasoconstriction in saline group
were more severe than Melatonin group (p-value = 0.009). Hemorrhage and leukocyte
infiltration were also more obvious in saline group (p-value 0.0018)
Conclusion: Our results demonstrated that Melatonin administration reduced ovarian
histopathological damage due to oxidative injury associated with torsion.
کليدواژگان: Melatonin, Saline, Ischemia, Reperfusion, Cat.
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| Association of inherited thrombophilia and antiphospholipid syndrome with severe preeclampsia
Hosseini MS, Gharehkhani P, Sadeghi M Page 183 (انگليسي)
Abstract مشاهده متن [PDF 100KB] | | Background: Severe preeclampsia is a quite well-known entity with high incidence of
both maternal and fetal morbidity and mortality. Although little is known about its
etiology، inherited disorders of hemostasis and antiphospholipid syndrome have been
postulated as common causes. The present study was conducted to evaluate the
association of these two entities with preeclampsia in a group of Iranian patients.
Materials and Methods: A case-control study was performed on 26 parturients with
severe preeclampsia and 26 healthy pregnant women who were matched according to
the age، parity، gestational age and previous history of abortion. A 10cc blood sample
was obtained and the following factors were measured: factor V Leiden، protein S،
protein C، antithrombin III، anticardiolipin antibodies (IgM and IgG) and the presence
of the lupus anticoagulant antibody.
Results: We have not found any significant difference in the values of factor V Leiden،
antithrombin III، protein C، protein S، and anticardiolipin-IgG between preeclamptic
(case) and non-preeclamptic (control) parturients. Meanwhile، lupus anticoagulant
antibody was detected in one case and one control. However، anticardiolipin IgM was
shown to be significantly higher in the preeclamptic patients. Severe preeclamptic
parturients were 4.4 times more likely to develop elevated levels of IgM (OR=4.4، 95%
CI=1.9-10، p<0.05).
Conclusion: Our results failed to reveal any significant association between
preeclampsia and indices of inherited disorders of hemostasis، except for anticardiolipin
IgM. Thus، routine screening of these indices are not recommended due to high
expenses and shortness of reliability.
کليدواژگان: Severe preeclampsia, Factor V leiden, Protein C, Protein S, Antithrombin III, Lupus anticoagulant
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| Comparing the levels of ßHCG, progesterone and estradiol between ectopic pregnancy and normal intrauterine pregnancy
Gharabaghi P, Abdollahifard S, Gharabaghi M, Nori M Page 187 (انگليسي)
Abstract مشاهده متن [PDF 42KB] | | Background: The value of serial measurement of serum ß subunit of human chorionic
gonadotropin (ßHCG) and ultrasonography in the early diagnosis of ectopic pregnancy
has well established.
Objective: The objective of this study was to explore the diagnostic value of raising
level of serum ßHCG، single measurement of progesterone (P) and estradiol (E2) in
early diagnosis of ectopic pregnancy.
Materials and Methods: Serum levels of ßHCG and estradiol were measured by Radio
Immuno Sorbent Assay (RIA) and progesterone level was measured by Enzyme Linked
Immuno Sorbent Assay (ELISA) techniques in 43 symptomatic women with ectopic
pregnancy and 42 women with normal intrauterine pregnancy in Alzahra Hospital،
Tabriz، Iran. These values were compared by T-test. By determining cut-off levels of
these parameters the efficiency and sensitivity of them in prediction of ectopic
pregnancy was estimated.
Results: The mean serum levels of ßHCG، estradiol and progesterone in patients with
ectopic pregnancies (940 ± 552 mlu/ml، 593 ± 237 pg/ml، 5.83 ± 3.41 ng/ml،
respectively) were significantly lower than these levels in normal intrauterine
pregnancies (4620 ± 2030 mlu/ml، 1627 ± 435 pg/ml، 24.8 ± 6.08 ng/ml، respectively).
The average rate of ßHCG rising was 8.2% for 24 hours in patients with ectopic
pregnancy (EP) and 32.8% in normal intrauterine pregnancies (NIUP).
Conclusions In this study single measurement of serum progesterone level has the
greatest sensitivity (100%) and specificity (98%) in the diagnosis of ectopic pregnancy.
کليدواژگان: Beta subunit of Human Chorionic Gonadotropin (ßHCG), Estradiol, Progesterone, Ectopic pregnancy,
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| The association of preterm labor with vaginal colonization of group B streptococci
Aali BSh, Abdollahi H, Nakhaee N, Davazdahemami Z, Mehdizadeh A Page 191 (انگليسي)
Abstract مشاهده متن [PDF 94KB] | | Background: Group B streptococcus is regarded as a potential factor for adverse
outcomes of pregnancy such as preterm birth.
Objective: To study the association of maternal vaginal colonization with group B
streptococcus (GBS) and preterm labor.
Materials and Methods: From April 2005 to May 2006، vaginal culture for GBS were
conducted in 101 laboring women with a gestational age of 24-37 weeks and 105
women admitted for term delivery at maternity center of Afzalipour Hospital in Kerman،
Iran. Student`s t test and Chi square test were used to compare continuous and
categorical data between the groups. Using multivariate logistic regression the
association between GBS colonization and preterm labor was analyzed. P-values<0.05
were considered as significant.
Results: Colonization was detected in 9.2% of all mothers. Although GBS colonization
was found more frequently in preterm than term patients (12 v/s 7 cases)، the difference
was not statistically significant. However، GBS positivity was roughly associated with
preterm labor. Age was also a risk factor for GBS colonization. No case of perinatal
sepsis occurred during the study period.
Conclusion: Maternal colonization for GBS is relatively low in our center. Increasing
age enhances the risk of colonization. Vaginal colonization of GBS is relatively
associated with preterm labor.
کليدواژگان: Group B streptococcus, Preterm labor, Vaginal colonization.
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| The relationship between passive smoking and ovarian response outcome in ART cycles
Owj M, Amirchaghmaghi E , Sadeghi M , Eshrati B , Shabani F Page 195 (انگليسي)
Abstract مشاهده متن [PDF 95KB] | | Background: Smoking has negative effects on reproductive process. Exposing to
cigarette smoking (passive smoking) may exert some effects as the direct smoking.
Objective: The aim of this study was to evaluate the correlation between ovarian
response and passive smoking in women who underwent ART cycles.
Materials and Methods: One hundred-sixty patients who underwent ICSI between
2000 and 2001 were studied in a prospective cohort study. The case group included
women whose husbands smoked at least 5 cigarettes daily for 1 year or more. The
control group included women with nonsmoking husbands. Women with high FSH
level (>12 IU/ml) were excluded. Long standard protocol with GnRH agonist and HMG
were used in all patients. In vitro fertilization and embryo transfer was carried out in a
standard fashion.
Results: Eighty one women were in case group and 82 in control group. Ovarian
response variables were not significantly different between two groups but there was a
significant relation between passive smoking and fertilization (RR= 1.18، 95% CI: 1.07-
1.31). However pregnancy rate was not significantly different between two groups.
Moreover there were no significant differences between heavy and light smokers in
ovarian response outcomes.
Conclusion: This study showed no correlation between ovarian response parameters
and passive smoking in women underwent ART cycles، whereas fertilization rate is
significantly lower in this group compared to control group. It may be related to sperm
quality than oocytes. Assessment of nicotin in follicular fluid and cytogenetic evaluation
of embryo before transfer are recommended for more information and confirmation.
کليدواژگان: Passive smoking, Ovarian response, In vitro Fertilization outcome.
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تاريخ انتشار: 10/11/86
تلفن: 8247085،8248348 (0351)
تاريخ درج در سايت: 20/11/86
شمار بازديدکنندگان اين شماره: 1005
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