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Archives of Advances in Biosciences - Volume:1 Issue: 1, Winter 2010

Archives of Advances in Biosciences
Volume:1 Issue: 1, Winter 2010

  • تاریخ انتشار: 1388/01/01
  • تعداد عناوین: 9
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  • Page 1
  • Shiva Kalantari, Seyedeh Zahra Moosavi, Nejad, Mostafa Rezaei, Tavirani, Seyed Hassan Moghaddamnia, Ashraf Shabani, Mehrnaz Mostafavi Page 2
    Adenosine Deaminase is an aminohydrolase (EC 3.5.4.4) which participates in the purine metabolism where it degrades either adenosine or 2''-deoxyadenosine producing inosine or 2''-deoxyinosine, respectively. The enzyme contains a parallel alpha/beta -barrel motif with eight central beta strands and eight peripheral alpha helices. ADA is located both in the cytosol and on the cell membrane. Since spermine, a natural metabolite, exists in all cells and tissues and its effect on the cell proliferation and enzyme regulation have been reported, thermal inactivation of the ADA and spermine regulatory effect on the ADA activity have been investigated in this study. Percentage of ADA activity in the presence and absence of spermine (1000 µM) in Tris buffer 50 mM, pH 7.5 at physiologic and pathologic temperatures have been reported in the present study. Thermal inactivation curves for ADA in the absence and presence of spermine (1000 µM) in different temperatures ranging from 55 oC to 70 oC have been drawn. Our data showed that spermine activates the enzyme in the low concentrations of adenosine at 37 oC. However, it inhibits ADA activity at 42 oC in the same concentrations of substrate. It is concluded that spermine regulatory effect depends on combined influence of temperature and adenosine concentration. Furthermore, thermal stability of the enzyme also depends on temperature in presence of spermine. Binding site of spermine on the enzyme has been identified by docking analysis.
  • Nasim Yarandi, Hakimeh Zali, Mohammad Ali Shokrgozar, Vahid Mansouri, Minoo Shahani, Amin Rostami, Saeid Heidari Page 9
    One of the prominent types of connective tissue cells is fibroblast that synthesizes and maintains the extracellular matrix of many animal tissues. Previous studies illustrated that calprotectin protein has different cytotoxicity effects on fibroblast cells. Calprotectin is abundant in the neutrophil cytosol; it has growth-inhibitory and apoptosis-inducing activities against various cell types such as tumor cells. The present study tries to introduce mechanism of growth inhibitory effect of calprotectin on human foreskin fibroblast cells (HFFF) and compare to etoposide (chemotherapy agent as control). Calprotectin was purified from human neutrophil by chromatography methods. HFFF cell lines were used, maintained in RPMI 1640 medium supplemented with 10% FCS in a humidified incubator (37 ºC & 5% CO2). The HFFF cells were exposed to the different concentrations of calprotectin and etoposide for 24, 48 and 72 hours. Cell proliferation was assessed by using dimethylthiazol diphenyl tetrazolium bromide assay. Flow cytometric analysis was performed to evaluate the cytotoxic mechanism of calprotectin on HFFF cells. Our results revealed that calprotectin and etoposide induce growth inhibition of HFFF in dose- and time-dependent manners. Sensitivity of HFFF cells to cytotoxic effect of human calprotectin was highly remarkable. In addition, growth inhibitory effect of this cytotoxic agent mostly was governed through induction of apoptosis in the HFFF cells. Taken together, calprotectin not only has more potent anticancer activity in comparison with the etoposide, but it also is an apoptosis inducer that acts on the proliferation of normal cells like fibroblasts.
  • Reza Rastmanesh, Akbar Faryar, Mostafa Eghbali, Youssef Shabani, Ehsan Mazinani, Ali Asghar Ebrahimi, Ahmad Reza Jamshidi Page 16
    Evaluation of functional disability by using proper instruments, like Stanford Health Assessment Questionnaire (HAQ) in patients with rheumatoid arthritis (RA), is necessary not only for quality of life assessment, but also as a useful marker for disease activity. Stanford HAQ has been translated into many languages. The present study seeks to measure reliability of the Persian version of the Stanford HAQ. Persian version of the Stanford HAQ (PE-HAQ) with culturally necessary modifications of the arising, eating, hygiene, reach and activities category questions was administered to 872 RA patients (87% female; mean age 57.5 years; mean onset age 51.5 years; mean disease duration 6.1 years). The arising, hygiene and activities scores were higher in the PE-HAQ than in the original Stanford HAQ, because futons and squat toilets are very common in Iranian culture and most of patients are bound to praying even in the standing position. Arising from a futon, squatting in a squat toilet and kneeling are generally more difficult for disabled individuals than are arising from a bed, getting on and off the toilet and doing chores, respectively. The overall disability index was higher in the PE-HAQ (0.89 ± 0.84) than in the original Stanford HAQ (0.82 ± 0.79), although the correlation coefficient was high (r=0.881). The test-retest reliability value studied at a 10-day interval, showed a strong correlation coefficient of 0.90 measured on the two occasions. PE-HAQ showed excellent internal consistency (Cronbach’s α= 0.892). The PE-HAQ is a valid and reliable instrument that can be self-administered to Iranian RA patients to evaluate their functional disability.
  • Sareh Zendehrouh, Fatemeh Bakouie, Shahriar Gharibzadeh, Amin Rostami Page 26
    Abnormal brain function in schizophrenia involves an extended network of brain structures. In schizophrenia, an abnormal dopamine activity in accordance with altered GABA and glutamate transmission appears to interfere with this process. In this study, we have examined the effect of dopamine hyperactivity on CA3 pyramidal cells using a mathematical model. Our simulation results show that while normal activity of dopamine system causes the membrane potential of pyramidal cell to display a periodic bursting behavior, hyperactivity of this system brings about irregular and aperiodic patterns of activity. In addition, it is suggested that hypo-glutamatergic conditions result in reduced activation of the striatal complex and may induce psychotic symptoms. Thus, we also investigated the role of glutamate level in postsynaptic cell activity. Simulation results indicate that hypo-glutamatergic condition has the same effect on the membrane potential of pyramidal cells, i.e. aperiodic and irregular firing patterns. Based on these results, we hypothesize that glutamate receptor activation may have good therapeutic results in schizophrenia.
  • Mostafa Rezaei, Tavirani, Hakimeh Zali, Ferdous Rastgar Jazii, Mohammad Hossain Heidari, Behrooz Hoseinzadeh, Salavati, Fatemeh Daneshi, Mehr, Kambiz Gilany Page 33
    Aldolase C as a glycolytic enzyme is associated with cellular structure at developmental stages of all cells, and this is particularly evident during the early stages of morphogenesis. It seems that expression of aldolase C can be regulated by the rate of differentiation that depends on the level of transcription or mRNA stability. There are several techniques to detect gene expression, here proteomics was used for determining expression of aldolase C (as a differentiation factor) in several cell types and basal cell carcinoma (BCC) tissue. The human astrocytes were differentiated from mesanchymal stem cells, fibroblast cells were cultured as primary cell culture and BCC tissue was taken from the patient. The fibroblast cells divided into two groups including sham and exposed groups. The exposed cells are them that were exposed to continue Extremely Low-Frequency Electromagnetic Fields (ELF-EMF). The analysis of 2DE gels, showed different expression of aldolase C in mentioned cells. The findings indicate that the amount of aldolase C expression decreases as differentiation process develops.
  • Ahad Khoshzaban, Saeed Heidari, Keshel, Sara Aghazadeh, Maryam Bashtar Page 40
    A functional treatment for skeletal damages in orthopedic and oral maxillofacial surgeries is required. Platelet growth factors such as Platelet Derived Growth Factor (PDGF), Bone Morphogenic Factor (BMP), Transferring Growth Factor-β (TGF-β) and Insulin-like Growth Factor-1 (IGF-1) proceed wound healing and bone regeneration. In the present study we focused on the effect of platelet rich plasma (PRP), platelet rich plasma gel (PRP-Gel) and auto bone chips on this process. 30 male, 22 weeks old, Sprague-Dawley rats weighing 525 g were used. They were divided in three groups consisting of PRP (treated by Platelet-Rich Plasma), PRP-Gel (treated by it), Bone chips and Control (two cavities created in each animal in this group). After 16 weeks they were histologically investigated while in the periods of 40, 60, 90and 120 days, the radiography had been done. The radiographic analysis showed complete treatment in all groups; however, by the histo-pathological investigations by auto bone chips complete and PRP-Gel partial healing has been observed. By histo-morphometric surveys (100±25) % in bone chips and (50±25) % in PRP-Gel groups bone bridging were observed, whereas in PRP it was not noticeable. The Present study suggests that neither PRP, nor PRP-Gel could be as beneficial as bone chips. Statistically, in PRP-Gel group, due to the existence of fibrin and thrombin, solid bone bridging at the treated site is indicated. According to the previous studies, in which the key role of both inhibitory and stimulatory signals in controlling the bone regeneration were proven, we suggest that auto bone chips could completely enhance healing due to signals among blood factors, environmental tissues and skeletal particles.
  • Parichehr Yaghmaei, Kazem Parivar, Davood Doranian, Mehrdad Hashemi, Fatemeh Torkaman Page 46
    Arterial diseases are the first causes of death in the developed countries. It seems necessary to investigate the role of enzymes, lipids, proteins and para-oxidation lipids in these diseases. The tribulation of fats is one of the most risk factors which results in atherosclerosis of coronary arteries. In this study, the short time effects of Extremely Low Frequency Electromagnetic Fields with the frequency of 50Hz, on the amount of some blood lipo-proteins, liver enzymes, P448 and P450 cytochrome enzyme system are investigated in the NMRI female mice. The results indicate that the blood serum''s levels of lipoproteins decrease significantly, in comparison with control and sham groups (P
  • Kambiz Gilany*, Luc Moens, Sylvia Dewilde Page 53

    Proteomics concerns itself with the characterization and function of all cellular proteins, the ultimate determinants of cellular function. Mass spectrometry has emerged as the preferred method for in-depth characterization of the protein components of biological systems. Using mass spectrometry, key insights into the composition, regulation and function of molecular complexes and pathways have been gained. Now days, mass spectrometry-based proteomics has become an indispensable tool in the cellular and molecular life sciences. This review discusses current mass spectrometry-based proteomics technologies.

    Keywords: Proteomics, Mass spectrometry