نشریه پلاسما و نشانگرهای زیستی
سال هفتم شماره 2 (پیاپی 26، بهار 1393)

Introduction & Biological scaffolds, composed of Extra Cellular Matrix (ECM), are capable of facilitating the restructuring of a large number of tissues. The objective of this study was to investigate the effect of ovine bladder scaffold of sheep, domesticated breedon blastema cells in vitro. At first, ovine bladder o the sheep was decellularized by putting in 1% wt/vol solution of sodium dodecyl sulfate (SDS) for 24 hours. Then to prepare the blastema tissue, the pinna of New Zealand rabbit was manually punched-hole with a special puncher and a circular blastema was separated. There after, the decellularized samples were put in the middle of blastema ring and were transfered to the culture medium. To continue the study, microscope with lights of hematoxylin & eosin and pick indigo and Toluidine blue staining was used. Some samples on the day 15 and 20 of culture were studied by transitional electron microscope. Collagen fibers were preserved after decellularized in matrix of bladder. Most migration of blastema cells occurred on days 15 and 20 of culture. On the day 10 and 15, some immature cells were differentiation in the pathway of adipocyte and fibroblast. On the day 15th and 20th, more blastema cells were migrated to the ovine scaffold. On the 10th and 15 day immature cell culture was seen to be differentiated from fibroblast and adipocyte cells. On the 15 th day blastema cell culture differentiated from covering cells and on the 20th day from fibroblast and adipocyte cells were seen. Bladder scaffold has ability to induce blastema cells and causes not only blastoma cells’ migration but also to be changed to different cells. This phenomenon is very important because of no growth factor and inducer was used in culture medium.

Inroduction & Prebioticdietary supplements that have the potential to reduce the harmful effects of infectious agents and have beneficial effects on host health. In this study, the prebiotic of yeast cell wall Saccharomyces cerevisiaat different levels to evaluate the blood indices and immune indices in rainbow trout juveniles were used.To conduct this study, 120 rainbow trout fry with a mean weight 3.61± 0.12 gr were randomly assigned into 12 fiberglass tub drainage volume of 100 ml distribution and breeding were given. An experiment to determine the optimum level of prebiotic effect of dietary yeast cell wall in the diet of rainbow trout fry in 1% prebiotic treatments, 1.5% prebiotic, 2% prebiotic prebiotic-free control, in a balanced design with three replications each were studied. At the end of the period rearing, blood indices and immune indices juveniles were studied.The results of some blood indices such as levels of White blood cells, Red blood cells, hemoglobin and hematocrit levels and neutrophil counts were not significantly different between the treatment and control groups were observed in 2% and other treatments (P0.05). The results showed that non-specific immune indices in blood, such as lysozyme and specific immune IgM and Ig statistically significant difference between treatment groups fed with 2 % prebiotic yeast cell wall with the other treatments and the control group (P Based onthese results it can be stated that the level of 2% prebiotic yeast cell wallused in thediet caused a significant increase in immune indices, andsomeblood parametersof studied, An important rolein immune function and improve blood indices of rainbow trout fry can play.

Introduction & Dexamethasone is a synthetic glucocorticoid that is used in medical sciences for treating many disorders suchovulation. Therefore, the present study investigates, the effect of prescribe of dexamethasone during pregnancy on function of pituitary-gonadal axis and number of ovary follicles of offspring of female rats. In this study, the researcher selected 40 pregnant rats that divided into control groups, sham group, and 3 experimental groups receiving 0.5, 1, and 2 mg/kg doses of dexamethasone, administered from the eighth day until the end of pregnancy QOD. After delivery, niether infants nor mothers received not no treatment. After puberty of female one, heart sampling was done and their ovary was separated and tissue section was taken, then plasma rate of strogen, progestron, FSH, LH and the number of ovary follicle were measured. Results were analysed with the SPSS software (V. 20) and ANOVA and Duncan test. (p≤0.05). Results of statistical analysisshowed that dose dependent dexamethson causes decreases estrogen, LH and increases progesterone, FSH and decreases the number of ovary follicle. Dexamethasone via inhibition of LH secretion and impairment of fetal ovarian development causes stable reproductive disorders in female rats.

Inroduction & This research was performed in cooperation with hatchery rainbow tout larvae,”research center of Persian drug shafagh”located in kharf village of Somesara town. Growth is one of the most complex phenomena that quantity, quality of food ingredients are effective. In order to reduce the risk of these diseases, you must increaselevel of fish resistance to infections by stimulation of immune such as vitamins.because abnormal levels of vitamin D are a random factor of many diseases,so food cycle,growth and immune system are cyclic chain that increase or decrease in above factors influenced on direct result of other factors.The Purpose of this study was the relationship between growth factors and immune system of fingerling rainbow trout during 8 weeks. This test was 120 fish with an average weight of 3 gr with 10 density in 12 tank fiberglass.This experiment did in 3 dietary treatments with 3 replicates and 1 control group did at concentration of 3000,4000,5000 international units of vitamin D3. This study showed that all growth factors at the end of experiment in the treatment were more than pther treatments and a significant difference was observed (P

Inroduction & Zander, Sander lucioperca, is an economical fish in the Caspian Sea. Due to the decline of stock in recent years, fry with different weights are released into the rivers by Iran Fisheries Organization. Studyonosmoregulatory function especially in developmental stages of larvae is essentialtorefinethe process offish into thefreshwaterorsaltwaterenvironments. Development of gill osmoregulatory Function, distribution and density of skin ionocytes were examined by histology with E & H staining and immunolocalization of Na, K-ATPase, in Caspian Sea zander (Sander lucioperca) larvae from day 1 to day 11 after hatching. Development of filaments was started from 4th days but immunopositive ionocytes were not observed in them. It was found weak immunofluorescence in filaments on the 8th days. The first buds of lamella were appeared with very poor immunofluorescence on the 11th days. Predominant distribution of the skin ionocytes was limited to the yolk sac membrane to 8 days after hatching and then tended to ventral region of posterior end of body. Skin ionocytes abundance and surface occupied by them increased from the first day to 11 days after hatching. In Caspian Sea zander larvae, development of osmoregulatory function of the gill had not been complete up to the 11th days and the skin ionocytes were responsible for osmoregulation until this age.

Inroduction & The different researches have been done to prevent population.Ricinus communishas different propertiesThis study analyzes the effects of preventing castor's generating. 21 male mice from NMRI species approximately weighing 20-30 gr classified into one control group and two experimental groups. Within 30 days, the experimental groups injected as 35 and 45mg/kg body weight by castor's hydro alcoholic extract and normal saline injected to the control group. 10 days after the mice were last injected, samples taken from their blood and generating organs.The serums transmitted into laboratory for the hormones assessments and some 5 micron transversal cuts taken from their testicles, next they were painted and spermcellular classification counted. The sperm maker's diameter pipes and the germinal layer measured by 10X lens and the cells counted by100X lens. All the data were done by ANOVA statistical method and Tukey test with the p≤ 0.05 meaningful level.A meaningful decrease observed in seminiferous tubules diameterpipes in the 45 mg/kg concentration,germinal diameter layer in experimental groups and the number of spermatogony, primary spermatocyte, sperm and leydig in experimental groups in comparison to control group(0.05>p). The percentage of the moving sperms, the percentage of the sperm's genetic substance and the sperm's morphology demonstrated a meaningful reduction in experimental groups and the hormones assesments.

Inroduction & Peptic ulcer, from histologicalpointisknownasmucosalnecrosisfactor. The purpose of this study was to investigate the effect of Alfalfa hydroalcoholicvegetivearial organs extract on gastric ulcer induced by acetic acid in Rat. In this experimental study, 60 wistar rats weighting approximately (200-250g) were randomly divided into 4 groups; control, sham(normal salin), experiment 1 (extract of dose 250 mg/kg body weight) and experiment 2 (extract of dose 500 mg/kg body weight). Rats were deprived of food and then underwent surgeryand gastric wasby injecting60% acetic acid after 4,7,10,14 days gavage, the rat's stomach out and then measuring the wound and fixed with 10% formalin, the passage of the tissue section was stained And the number of neutrophils, macrophages and fibroblasts were counted.Results showed that the hydroalcoholic extract of Alfalfacaused a significant increase in gastric ulcer healing in experimental groups 1 and 2 (PAlfalfahydroalcoholic vegetive arial organs extract is effective in the treatment of peptic ulcer induced by acetic acid.

Inroduction & Peptic ulcer, from histologicalpointisknownasmucosalnecrosisfactor. The purpose of this study was to investigate the effect of Alfalfa hydroalcoholicvegetivearial organs extract on gastric ulcer induced by acetic acid in Rat. In this experimental study, 60 wistar rats weighting approximately (200-250g) were randomly divided into 4 groups; control, sham(normal salin), experiment 1 (extract of dose 250 mg/kg body weight) and experiment 2 (extract of dose 500 mg/kg body weight). Rats were deprived of food and then underwent surgeryand gastric wasby injecting60% acetic acid after 4,7,10,14 days gavage, the rat's stomach out and then measuring the wound and fixed with 10% formalin, the passage of the tissue section was stained And the number of neutrophils, macrophages and fibroblasts were counted.Results showed that the hydroalcoholic extract of Alfalfacaused a significant increase in gastric ulcer healing in experimental groups 1 and 2 (PAlfalfahydroalcoholic vegetive arial organs extract is effective in the treatment of peptic ulcer induced by acetic acid.

Inroduction &

Vitamins are the most important nutrients and vitamins that stimulate the immune system of fish. vitaminC on Aquaculture is considered as an essential nutrient. The purpose of this study was to evaluate different levels of vitamin C from L - ascorbyl -2 - polyphosphate on blood factors and immune factors Siberian sturgeon.

Levels of the vitaminC of type L - ascorbyl 2 - polyphosphate include: lack of vitaminC (Treatment 1), 100 mg/kg vitaminC (Treatment 2), 200 mg/kg vitaminC (Treatment 3), 400 mg/kg vitamin C (Treatment 4), 800 mg/kg vitamin C(Treatment 5), 1600 mg/kg vitaminC (Treatment 6) in triplicate and grown for 8 weeks was considered the360numberof Siberian sturgeon. At the end of the experiment, blood and immune parameters were measured ResultsResearch results on farmed Siberian sturgeon of the blood and immune factors showed the highest number of red blood cells (RBC) In the treatment of 200 mg/kg vitaminC was observed with the other treatments showed significant differences. While the difference Ing the number of white blood cells (WBC), Hematocrit and hemoglobin were observed, but most of them treated 200 mg/kg vitaminC was common's other hematological indices MCH and MCHC Statistically significant difference in between treatments were found in the MCV There was no significant difference between the treatment and the highest 1,600 mg/kg vitaminC common and Siberian sturgeon studies on blood safety parameters showed no significant difference in the amount of lysozyme, IgM In between treatments, but there is no significant difference between treatments in the total immunoglobulin and lysozyme and immunoglobulin in the treatment of 800 mg/kg vitaminC was higher than other treatments.

The absence of vitaminC In the diets of factor reduces safety, the results showed that the factor safety in treatment the high vitaminC showed better effect.