نشریه پلاسما و نشانگرهای زیستی
سال هشتم شماره 4 (پیاپی 32، پاییز 1394)

Inroduction & Cancer is dynamic process which causes molecular changes and interference in cell proliferation system by numerous independent variables, breast cancer involves such changes so that it has the highest rate of cancer among women.The purpose of this study is studings the anticancer effect of Nigella seeds extract in breast cancer,so that the effect of nutrition with Nigella seeds oil on ativity of antioxdant enzymes in splees of mice effected by breast cancer is studied.Four group of Balb/c mice are divided to one control group and 3 traetment groups,in control group, Gavage was done by distilled water and in three other groups, gavage was done by nigella seeds oil with dosages of 1,2,4 mg/Kg body weight for 4 months,then the mice received tumor pieces through operation intradermally,Tumor volume was studied by V= LW2/2 and it's dimensions were measured by digital caliper. The results of this study indicated that the average volume of tumors of mice treated with Nigella seeds oil with dosage of 4mg/kg body weight indicated a significant difference compared to control group in data analysis,as well as the rate of vessels congestion in mice treated with Nigella seeds oil with dosage of 4 mg/kg body weight indicated a meaningful reduction. Probaby,Nigella seeds oil has protective effect against cancer cells with decrease cancer cell and vessels congestion and increase appotes.

Inroduction & Probiotics are live microorganisms that with the improvement of intestine microbial balance, enforce useful impacts on the host (human or animal) and are used as food supplements or pharmaceuticals. Today production of Probiotic products are worldwide.Functional charectristics of these microorganisms include: adjustment of immune system, decrease of cholesterol serum, digestive infections, cancer, dermal and food allergies in kids, vesica and ear infections and decrease of chronic and travel diarrhea. Researches have shown that by means of vagus nerves, food can impact on the brain and nervous hormones, so it is probable that with regulation of the action of brain’s different parts, probiotics can impact on the improvement of its function. In this research 40 male vistar rats with the average weight of 150-170 g and approximate age of 4 weeks were chosen for the investigation of the usage of these probiotic’s effects, and were placed accidentally in 4 groups and were exposed to stress for 4 weeks and treatment with bacterium T4 Daily. The usage of probiotic bacterium T4 after 4 weeks has an effect on the decrease of stress in mice in a sensible manner(p<0/05).

Inroduction & The protein source of high quality protein supplements commonly used in community sport. The aimof this study was to investigate the effect12 sessions of circuit resistance training with whey protein on apolipoprotein M- HDL cholesterol levels and lipid profile in non-athletic men. This type of application and research of quasi-experimental design with a control group and the group receiving whey protein (50 gr) that immediately after each practice was given orally to the subjects, respectively. The training program for 12 sessions follow: three sessions in each week with one hour circuit resistance training in each session. Data analyzed using SPSS-18 and independent and dependent T student tests and the used a significance level of 0.05. Significant decrease of apolipoprotein M- HDL total cholesterol level and HDL3 cholesterol were observed in comparison with prior phase (P=0.01). Significant increase of HDL2 cholesterol was observed circuit resistance training group comparison with prier phase (P=0.03). This study showed that circuit resistance training affecting but positive effects have on the lipid profile changes in untrained young men.

Inroduction & Colorectal cancer is the second most common cancer in the world Domain genetic changes in the development of colorectal cancer showed a large. Infection is a particular type of E. coli bacteria in patients with inflammatory bowel disease, particularly ulcerative colitis, and colorectal cancer can begin. Certain types of bacteria Escherichia coli siderophore production of toxins disrupt the cell cycle and begin the development of colorectal cancer, the aim of this study is the relationship between strains of E. coli siderophore and colorectal cancer patients. Biopsy of the large intestine The clinic includes 35samples from colorectal cancer patients and 35samples from healthy individuals and colorectal cancer were intestinal diseases. Smears of E. coli isolated from healthy tissue and cancer tissue, and DNA extraction by Duplex PCR technique for two genes, feoB and fyuA, as a marker genes siderophore was done. The study of 70patients with colorectal cancer and individual health clinic of intestinal biopsy was obtained and E. coli were isolated from cancerous tissue %59.3 gene feoB and %20 of healthy tissue and gene fyuA %14.8 in patients and %8 were observed in healthy individuals. The results of the genetic study published in Iran similar to the results in many European countries show less.

Inroduction & Prebiotics, as a non-digestible food ingredient, beneficiallyaffects the host by selectively stimulating the growth, activating a limited number of bacteria, andreducing the number of harmful bacteria in the gastrointestinal tract.This study investigated the effects of xylooligosaccharide on digestive enzymes activity, body composition and intestine bacterial flora of sobaity (Sparidentex hasta.For this purpose, 405 individuals of sobaity fingerlings (45 in each replication) were prepared with an average weight of 7.64 ± 0.3 g from the Mariculture Research station of South Iranian Aquaculture Research Center. This study was carried out in a completely randomized design with three treatments and replications in fiberglass tanks with 300 liters volume. Fish were fed with feed containing 0, 0.5 and 1 percents of xylooligosaccharide for a period of 42 days. At the end of the experiment, body composition and intestine samples were collected for estimating digestive enzymes activity intestine bacterial flora and body composition. The obtained results indicated that dietary xylooligosaccharide did not change sobaity digestive enzymes activity, including alkaline protease, amylse and lipase (P > 0.05). In this study, weak, positive and no significant correlation were observed between dietary prebiotic and alkaline protease activity, amylase activity and lipase activity (P > 0.05). The results of this study indicated that different levels of prebiotic did not affect body composition including protein, fat, ash and moisture and intestine bacterial flora (P > 0.05). Overall, this study showed that digestive enzymes activity was not affected by dietary prebiotic. Moreover, the diet supplemented with 0.5 and 1 percents of xylooligosaccharide had no significant effects on body composition and intestine bacterial flora of sobaity

Inroduction & Present study was carried out in collaboration with culture and propagation section of international research institute of Sturgeon fishes during fall 2014. Growth is one of the most complicated Phenomenons that food quality and quantity as well as ingredient of food are effective in it. Fish resistance level to infections should be improved using immunestimulator such as vitamins to reduce disease risks in fish. Since abnormal levels of vitamin D and Calcium (Ca) is a accidental factor in most of the diseases; so food chain, growth and immune system are a chain cycle that increasing or decreasing of one of them affect to others directly. The aim of present study was to show that there is direct connection between growth parameters and immune system in Acipenserpersicus during 8 weeks.60 fingerlings with mean weight of 18.56 g were divided in to fifteen 50 L tanks each contain 4 fish. 4 experimental diets and 1 control group were designed with three replications. Fish in treatment 1, 2, 3 & 4 feed by 6g/Kg Ca, 3000 IU D, 12 g/Kg Ca and 6g/Kg Ca + 3000 IU D respectively. Fish in control group feed by basal diet without Ca and D. fish weight and length were measured once 2 weeks and growth parameters were calculated. Blood parameters were measured at the end of experiment. Results showed that all growth parameters in treatments1 and 2 were significantly more than other groups (P 0/05).

Inroduction & Colorectal cancer (CRC) is the third most common cancer in men and the second most common cancer in women in the world. Axin gene includes axin1 and axin2. The C-myc and other genes of this family encode transcription factors that play a vital role in cell proliferation, growth, differentiation and apoptosis. The aim of this study was to evaluate semi-quantitative genes expression of AXIN1,CMYC in mRNA tissue of Iranian patients with CRC and checking the communication ON or OFF of this genes in each tumor stages and pathologic factors of patients.Therefore, we have done RT-PCR with specific primers for Axin1,CMYC and β-actin genes on the 54 tumor and 41 normal tissues as fresh samples which obtained from 54 unrelated patients referred to Imam Khomeini Hospital. According the results, 55% of the samples were ON and 45% of the samples were OFF for AXIN1 gene expression and 87.5% of the samples were ON and 12.5% of the samples were OFF for CMYC gene expression respectively. Statistical analysis with SPSS, T-test and X2 showed that there is a significant differences of the Axin1 and CMYC expression between two groups of samples; normal, tumor(0/05P). According the results, 55% of the samples were ON and 45% of the samples were OFF for AXIN1 gene expression and 87.5% of the samples were ON and 12.5% of the samples were OFF for CMYC gene expression respectively. Statistical analysis with SPSS, T-test and X2 showed that there is a significant differences of the Axin1 and CMYC expression between two groups of samples; normal, tumor(0/05P)

Inroduction & Breast cancer is the most common malignancy in women worldwide including Iran. Several studies have demonstrated anti-cancer and apoptotic properties of phycobili proteins from different strains of cyanobacteria phycocyanin. For the first time the aim of this study was to investigate anti-cancer effects of Anabaena sp. ISC55 phycocyanin and to compare with paclitaxel، on breast cancer 4T1cell line. After culturing cyanobacteria، phycocyanin was specifically extracted. 4T1 breast cancer cells were cultured. Colony forming assaywas performed and cytotoxicity of different concentrations (250- 200- 175-150- 125-100- 75-50- 25-20- 15-10) mg/ml of phycocyanin and paclitaxel was tested by MTT assay and adhesion test after 24 hours incubation with cultured cells. The effects of these concentrations on the morphological features of cells was evaluated under inverted microscope. Data were analyzed using one-way ANOVA followed by Tukey''s multiple comparison test as post hoc. The results indicate an increase in anti-cancer properties phycocyanin in high concentrations by increasing apoptosis with a significance level of P<0/001. According to the results، Anabaena sp. ISC55 phycocyanin has dose dependent anti-cancer properties.