فهرست مطالب

Blood and Cancer - Volume:10 Issue: 2, Jun 2018

Iranian Journal of Blood and Cancer
Volume:10 Issue: 2, Jun 2018

  • تاریخ انتشار: 1397/03/30
  • تعداد عناوین: 8
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  • Shafagh Rostami, Mojgan Pourmokhtar * Pages 35-38
    Background
    Despite the availability of a wide range of antibiotics, bacterial infections are among important challenges for the healthcare system. Therefore, the introduction of new antimicrobial preparations such as platelet-rich plasma (PRP) could be suggested theoretically as a preventive measure for bacterial infections. We aimed to evaluate the in vitro antibacterial activity of PRP against three common oral bacteria.
    Methods
    In vitro antibacterial activity of PRP against Streptococcus Mitis, Streptococcus Mutans, and Neisseria Lactamica as three common oral/dental bacteria was determined by disc diffusion method. Baseline antibacterial activity was assessed by measuring the diameter zone of inhibition after 24 hours of incubation at 37 °C.
    Results
    PRP had strong in vitro antibacterial activity against Streptococcus Mitis, Streptococcus Mutans and Neisseria Lactamica with the mean zone of inhibition diameters of 6.73±0.52, 5.8±0.43 and 6.67±0.43 mm, respectively.
    Conclusion
    PRP is an effective antibacterial agent along with conventional antibiotic treatments against oral and periodontal infections.
    Keywords: Platelet, rich plasma, Antibacterial activity, Oral, periodontal infections, Antibiotic treatment
  • Saleh Nasiri *, Keimer Teke, Kamran Mousavi Hosseini Pages 39-42
    Background
    The short life time of human platelet units has led to a chronic shortage of fresh platelets in blood transfusion centers. Many approaches have been investigated experimentally to produce new hemostatically active platelet products that are capable of long term storage. Infusible platelet membrane (IPM) prepared from fresh or outdated human platelets have been developed as an alternative to standard platelet concentrates with the additional advantage of long shelf life. We aimed to measure the presence of CD41/CD61, CD42b platelet receptors and platelet factor 3 (PF3) activity during IPM preparation.
    Methods
    After pooling of fresh platelet concentrates, freeze-thawing, washing, formulation and lyophilization steps were performed.
    Results
    Flow cytometric analysis of CD41, CD61 and CD42b after lyophilization of IPM found to be 45.9%, 51.3% and 44.4% with PF3 activity of 39% in contrast to 81.5%, 87.5% and 81.1%, respectively on fresh pooled platelet concentrates with 100% PF3 activity.
    Conclusion
    The results showed that lyophilized IPM product can preserve major adhesion (CD42b) and aggregation (CD41/CD61) platelet receptors and may retain some PF3 activity at acceptable level which demonstrates hemostatic property of the lyophilized IPM.
    Keywords: Infusible platelet membrane, CD41, CD61, CD42b receptors, Platelet Factor 3, Platelet substitute, Platelet adhesion, Platelet aggregation
  • Ziba Asadpoor Dezaki, Maryam Kheirandish * Pages 43-49
    Background
    In recent decade, human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) provide enormous potential for appropriate cell therapy, but they have limited growth potential and cease to proliferate due to cellular senescence, so providing a strategy for increasing the stem cell survival is necessary.
    Methods
    In this investigation, MSCs characterized by flow cytometry were isolated from umbilical cord blood. Hypoxia preconditioning (HPC) was induced in a water-saturated gas mixture of 2.5% O2 and 5% CO2 for 15 min and then reoxygenation at 21% O2 for 30 min at 37 °C. Subsequently, hypoxia preconditioned hUCB-MSCs were exposed to 2.5% O2 and 5% CO2 for 24, 48 and 72 hr (HPC hypoxia groups). We examined the proliferation capacity of hUCB-MSCs after HPC in comparison with normoxia status, and we determined the best duration time of being under hypoxia (24, 48 or 72 hr of hypoxia). In order to assess the role of HPC on the expression of surface markers, cells were analyzed by flow cytometry. Proliferation of cells was evaluated using MTT assay, and doubling time and colony-forming unit-fibroblast (CFU-F) was calculated in each group.
    Results
    The MTT results showed that cell viability of HPC-UCB-MSCs significantly increased in comparison with UCB-MSCs under normoxia condition. Our study revealed that HPC reduces the doubling time of UCB-MSCs remarkably after passaging 48 hours of hypoxia. Our results proved that HPC can significantly increase the CFU-F colony numbers of hUCB-MSCs without any alteration on cell surface marker expression.
    Conclusion
    Our results suggested that HPC of umbilical cord blood derived mesenchymal stem cells along with induction of hypoxia can provide a suitable culture condition for rapid proliferation of mesenchymal stem cells with no effect on their immunophenotype features and it could be a potential therapeutic option for increasing the capability of MSCs.
    Keywords: Cell proliferation, Cell survival, Hypoxia preconditioning, Mesenchymal stromal cell, Umbilical cord blood, Surface marker expression, Tissue differentiating capability
  • Maryam Masaeli, Abdulrahman Bahrami, Masoud Shahabian* Pages 50-55
    Background
    Benzene has been classified as group 1 human carcinogens in the environment by the International Agency for Research on Cancer (IARC) mainly because of its ability to cause acute myeloid leukemia (AML). This study was conducted to detect any probable association between urban benzene pollution concentration and occurrence of AML in different regions of Tehran.
    Methods
    In this descriptive study, demographic data of AML cases recorded in cancer registry centers of Tehran city since March 2006-2011 who met the inclusion criteria were extracted. Eligible criteria included residing in Tehran city for at least 5 years prior to diagnosis of AML. Collected data were correlated with concentration of benzene in ambient air available since March 2001 to March 2011.
    Results
    The majority of AML subjects were male in the age range of 20-30 years. Maximum concentration of benzene was reported from southern (11.35-106.57 ppb) and central (5.5-60.18ppb) regions of Tehran. The association between benzene concentration with occurrence of AML was observed significant for the period between March 2010 to March 2011 for male patients (Pearson correlation=0.906, P=0.05) in the age range of 30-40 years (Pearson correlation=0.893, P=0.041).
    Conclusion
    This study suggested probable evidence for the association between the incidence of AML and concentration of benzene in southern and central areas of Tehran city.
    Keywords: Ambient air, Acute myeloid leukemia, Benzene concentration, Cancer, Environmental exposure
  • Morteza Bagheri, Isa Abdi Rad *, Davood Maleki, Ali Eishi, Nasim Valizadeh Pages 56-60
    Background
    The Philadelphia chromosome (Ph) characterized by t (9; 22) (q34; q11.2) is a reciprocal translocation giving rise to a chimeric BCR-ABL fusion gene. Incidence of Ph chromosome is over 98% in Patients with Chronic Myeloid Leukemia (CML) and around 20% in acute lymphoblastic leukemia (ALL). The finding of this fusion gene is essential for diagnosis of CML by detection of various fusion transcripts such as b2a2 and b3a2 transcripts and Ph positive ALL by detection of e1a2 (p190) transcripts. We conducted this study to determine the frequency of various BCR-ABL fusion transcripts in the west Azerbaijani patients with CML.
    Methods
    RNA was isolated from peripheral blood samples by standard protocols. BCR-ABL fusion gene detection was carried out with one-step multiplex RT-PCR in 41 west Azerbaijani patients with CML.
    Results
    Among patients with CML, the frequencies of b2a2 and b3a2 transcripts were 52.5% and 12.5%, respectively. Co-expression of b3a2 and b2a2 transcripts was found in 12.5% of the patients.
    Conclusion
    The findings of this study showed that multiplex RT-PCR is a suitable technique to identify the typical BCR-ABL fusion transcripts in the west Azerbaijani patients with CML. Atypical transcripts possibly run away while using multiplex PCR.
    Keywords: BCR, ABL fusion gene, CML, a2b2 transcript, a2b3 transcripts, RT, PCR test
  • Saylan Mohammadi, Mostafa Moghaddam, Serveh Babahajian, Mohammad Saied Karimian, Shirin Ferdowsi * Pages 61-63
    Detection of ABO blood groups through agglutination is the basis of pre-transfusion testing. However, weak agglutination reactions may be obtained with reagent antibodies and are a result of weak expression of A and B antigens on red blood cell surface which may cause a discrepancy in blood group typing. Here, we report a donor which showed discrepancy between red blood cells (forward) and serum (reverse) typing. A detailed analysis revealed the blood type as a variant of blood group B. Subgroup of B is a very rare phenotype of blood. This is the first case ever detected in Kurdistan blood transfusion Center.
    Keywords: Discrepancy, Blood grouping, B subgroup, Forward typing, Reverse typing
  • Afshin Karami, Mehrdad Payandeh, Noorodin Karami *, Amir Yami Pages 64-65
  • Parastou Molai Tavana, Samin Alavi * Pages 66-67