فهرست مطالب

Gene, Cell and Tissue - Volume:3 Issue: 4, Oct 2016

Gene, Cell and Tissue
Volume:3 Issue: 4, Oct 2016

  • تاریخ انتشار: 1395/08/13
  • تعداد عناوین: 7
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  • Ahmad Shabanizadeh, Mohammad Mohsen Taghavi, Mahdi Shariati Kohbanani, Zahra Taghipour, Hamidreza Jafarinaveh, Akram Mollahosseni, Mohammad Reza Salahshoor*, Cyrus Jalili Page 2
    Background
    MTHFR gene is one of the main and effective factors in genes methylation. This gene has a common polymorphism in cordon 677, which can affect its activity. Changing activity rate of this gene can affect methylation rate of tumor suppressor genes and cell membrane proteins and other genes in addition to their expression rate. Therefore, it can be considered as one of the effective factors on producing cancer.
    Methods
    In this descriptive-cross sectional study, 34 cancer and non-cancer tissue samples were studied. Initially, DNA was extracted from samples and then E-cadherin and MTHFR c677t polymorphism were amplified by the polymerase chain reaction (PCR). Methylation status of E-cadherin was evaluated by adding methylation restriction enzyme HpaII and polymorphism of MTHFR c677t was assessment by the restriction fragment length polymorphism (RFLP) method.
    Results
    Methylation status of E-cadherin gene numbers of methylated cases in the cancer group were equal to one and unmethylated cases equal to 33 and numbers of unmethylated cases in the non-cancer group was equal to 34 while there were no methylated cases. In assessment of methylation status of E-cadherin and different genotypes of MTHFR in codon number 677 in cancer samples, number of CC, CT and TT genotypes were equal to 0, 1 and 0 in the methylated group and equal to 6, 27 and 1 in the unmethylated group, respectively.
    Conclusions
    According to this observation, another factor such as oncogenes activity may cause cancer in samples but CT genotype of MTHFR can be considered as an effective factor in creating cancer.
    Keywords: Gastric Carcinoma, Methylation, E, Cadherin, MTHFR
  • Zahra Farzaneh Taban, Shiva Khatibi, Raheleh Halabian, Amaneh Mohammadi Roushandeh* Page 3
    Background
    Mesenchymal stem cells (MSCs) survival decreased following in vivo injection and its application has been limited in stem cell therapy. Preconditioning was established as a strategy to increase the cells’ efficiency, but more studies are necessary to determine its safety and mechanisms.
    Objectives
    The aim of this study was to evaluate the effect of preconditioning mesenchymal stem cells with low serum and H2O2 on in vitro cell survival.
    Methods
    Mesenchymal stem cells were cultured and preconditioned with low serum and H2O2 for 6, 12, 24 and 48 hours in six groups. Control, group I with 5 μM H2O2, group II with 10 μM H2O2, group III with 0.5% fetal bovine serum (FBS), group IV: 5 μM H2O2 0.5% FBS and V: 10 μM H2O2 0.5% FBS. Cell proliferation was evaluated with the MTT assay.
    Results
    Cell proliferation in groups IV and V increased significantly compared to the other groups after 6, 12 and 24 hours of treatment (P
    Conclusions
    Our data suggest that preconditioning with 5 μM H2O2 0.5% FBS and 10 μM H2O2 5% FBS improved cell proliferation and viability. However, the mechanisms related to protective properties of preconditioning and using this strategy in stem cell therapy requires more research.
    Keywords: Mesenchymal Stem Cell, Proliferation, Cell Survival, Preconditioning
  • Mohammad Zandi*, Majid Masoumian, Asghar Shariatinia, Mohammad Rez Sanjabi Page 4
    Background
    Many studies examine the antibacterial effects of medicinal plants; however, little research is done to evaluate their effects on different cell types, especially dermal fibroblasts.
    Objectives
    The current study aimed to study the effect of different concentrations of Aloe Vera, henna, chamomile, myrtle, mint, licorice, cinnamon, ginger and cedar extracts and their synergistic effects on the viability of dermal fibroblasts.
    Methods
    To evaluate the performance of herbal extracts on dermal fibroblasts, in the first experiment different concentrations (6.25, 12.5, 25, 50, 100, 250, 500 and 1000 µg/mL) of the extracts were evaluated by the MTT cell proliferation assay. In the second experiment, the minimum effective concentrations of the plant extracts in triple combination were evaluated in the cells under study.
    Results
    The minimum effective concentrations of henna, chamomile, myrtle, mint, cinnamon, ginger and cedar were 12.5, 6.25, 6.25, 6.25, 6.25, 12.5 and 12.5µg/mL, respectively. Results showed that, by comparing the minimum effective concentration of herbal extracts, the viability of dermal fibroblasts significantly increased by cedar extract (P
    Conclusions
    Based on the results of the current study, it was concluded that Aloe vera, licorice and mint extracts had synergistic effects on the viability of dermal fibroblasts; in addition, the combination of Aloe vera and licorice with either henna or myrtle, and Aloe vera and mint with either cedar or ginger resulted in synergistic effects on viability of dermal fibroblasts. The third category of triple combinations of herbal extracts with synergistic effects on the cells under study was the combination of Aloe Vera and mint with either chamomile or cinnamon and also Aloe vera and licorice with either myrtle or cedar.
    Keywords: Herbal Extracts, Dermal Fibroblasts, Viability
  • Hamidreza Mahmoudzadeh Sagheb, Zahra Heidari, Mehdi Jahantigh, Mahdieh Narouei* Page 5
    Background
    Helicobacter pylori (H. pylori) infection is considered as a carcinogen for gastric mucosa. The mechanism by which H. pylori are involved in gastric carcinogenesis is still unclear.
    Objectives
    The current study aimed to investigate the effects of H. pylori infection on immunohistochemical expression of p53 and Ki-67 genes in gastric specimens of patients with intestinal metaplasia (IM), dysplasia (DYS) and gastric cancer (GC).
    Methods
    In the study, 42 cases with IM, 38 with DYS and 42 with GC were selected from pathology archive of Ali-ebne-Abitaleb hospital, Zahedan, Iran; p53 and Ki-67 immunohistochemical study was done, accordingly. Kruskal-Wallis and Mann-Whitney U tests were used to compare the groups. Level of significance was set at P
    Results
    In IM specimens, expression of p53 and Ki-67 was significantly higher in the cases with H. pylori infection than those not infected with H. pylori. In DYS specimens, in the group with H. pylori infection, the expression of p53 was significantly higher while expression of Ki-67 was significantly lower than the group without H. pylori infection. In GC specimens, the expression of p53 was significantly higher in the group with H. pylori infection compared to the group without H. pylori infection. Expression of Ki-67 in the specimens with H. pylori infection was not significantly different from those without H. pylori infection.
    Conclusions
    The study results showed that p53 expression increased in IM, DYS and GC cases with H. pylori infection compared to those without H. pylori infection. The results also suggested that, Ki-67 expression was different in precancerous stages of gastric carcinogenesis, based on the infection with H. pylori.
    Keywords: Immunohistochemistry, Gastric Cancer, p53, Ki, 67, Helicobacter pylori
  • Saeed Khosropour, Maryam Shojaee*, Peyman Lotfi Page 7