فهرست مطالب

Biological and Biomedical Journal - Volume:2 Issue: 2, Spring 2016

International Biological and Biomedical Journal
Volume:2 Issue: 2, Spring 2016

  • تاریخ انتشار: 1395/07/18
  • تعداد عناوین: 6
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  • Ali Mirzapour, Fatemeh Shahnavazi, Ahmad Karkhah, Seyed Reza Hosseini*, Hajighorban Noreddini, Ali Bijani Pages 53-59
    Investigations regarding the relationship between bone mineral density (BMD) and thyroid function tests even in the normal range are limited. Therefore, the present study was conducted to investigate the relationship between BMD with thyroid function tests in elderly people. This study is a part of a prospective study about health status of the elderly people in Amirkola city (AHAP) which is ongoing from 2011. A total of 1178 people (655 men (55.6%) and 523 women (44.4%)) were enrolled in this study and the TSH, T4 and T3 levels were measured by ELISA method. BMD was assessed by Dual X-ray Energy Absorptiometry (DEXA). Our results demonstrated a significant positive correlation between total T3 and TSH levels with femur density in women (r= 0.068, P= 0.019 and r= 0.122, P= 0.005, respectively). There was also a negative correlation between T4 levels and femur density in men (P=0.034, r= -0.083). Obtained results showed a significant direct correlation between normal levels of TSH and femoral density in elderly females but not in normal elderly males. In addition, there was no significant correlation between normal TSH levels and density of lumbar region in both males and females.
    Keywords: Thyroid function, osteoporosis, TSH, elderly people, bone mineral density
  • Nafise Taheri, Ali Akbar Samadani *, Lale Vahedi, Seyedeh Elham Norollahi, Parviz Yazdani, Maryam Ghasemi, Ramin Ramazanpour Pages 60-66
    Skin cancer is one of the most important type of cancers in the world. In this way, molecular investigation in order to detect some novel mechanisms and polymorphisms involved in cancer development can be impressive and vital. In this way, the aim of this study was the histopathological investigation of skin cancer and its relationship with polymorphism of CDKN2A gene. This case-control study was conducted in north of Iran and 36 samples of squamous cell carcinoma (SCC), 64 cases of basal cells carcinoma (BCC) and 50 healthy control samples were investigated. Histopathological and tissue structures were evaluated after hematoxylin and eosin staining, under light microscope. In addition, investigating the CDKN2A gene polymorphism was carried out by using PCR_sequencing. 58% of patients were male. The mean age of patients was 63.69 years and the average tumor size was 3.57 mm. No CDKN2A gene polymorphism was observed in intron 1. The investigation of other DNA repair genes can help to better understanding of genetic factors predisposing to skin cancer development.
    Keywords: Histopathology, polymorphism, CDKN2A gene, basal cell carcinoma, squamous cell carcinoma
  • Olarinde Olaniran*, Ayodele.J. Osevwe, Abiodun.I. Balogun, Olusegun.T. Afolabi, Rachel.E. Hassan-Olajokun, Olufemi.O. Oyetoke, Ayobami.A. Awoyeni Pages 67-72
    The Identification and evaluation of Trichomonas vaginalis among women was performed over a period of three months in Ilesha. The aim of this study was to determine the prevalence of Trichomonas vaginalis in women in relation to different samples collected and level of education. 310 samples from high vaginal, endocervical and urethral regions of female patients were analyzed using Gram staining and direct microscopy. 25 samples (8.1%) were positive for Trichomonas vaginalis. Out of the positive samples, 52%, 48% and 0% were collected from high vaginal, endocervical, and urethral regions, respectively. Age group 16-25 years had the highest prevalence: 15 (60%), followed by 26-35 years: 8 (32%), and the least represented age group correspond to 6-15 years: 2 (8%). Less educated women had the highest prevalence of Trichomonas vaginalis infection with 15 (60%) cases while 10 (40%) cases were present in educated women. The prevalence of Trichomoniasis in the present study was high, but there was no significant difference in overall distribution of the parasite in high vaginal or endocervical swabs. Therefore, either of the samples can be used for investigating Trichomonas vaginalis infection in patients presenting the symptoms.
    Keywords: Prevalence, Trichomonas vaginalis, high vagina swab, endocervical swab, urethral swab, Nigeria
  • Mohamed Abdelsalam Ismail, Waleed Sayed Koko, El-Badri Elamin Osman, Mahmoud Mohamed Dahab, Mohammed Ismail Garbi, Alsadeg Mohamed Alsadeg, Ahmed Saeed Kabbashi* Pages 73-79
    The Acacia seyal Dell family Mimosaceaeis is known locally as Talha tree. Many medicinal uses such as treatment of dysentery, leprosy, colds, diarrhea, hemorrhage, ophthalmic, intestinal ailments, rheumatic pains, colds and fevers, have been attributed to this tree. Schistosomiasis, a water-borne parasitic disease caused by Schistosome(s) a genus of digenetic trematode worms, infects man and animals in tropical and sub tropical regions. It is the second most prevalent parasitic disease in the world ranked only after malaria regarding the number of people infected and those at risk. More than 90% of the cases are occurring in Africa. The purpose of this study was to investigate the molluscicidal activiat ty of Acacia seyal bark methanolic extract against the snail Biomphlaria pfeiffer. Different concentrations (1000, 500, 250, 125, 62.5, 31.25, 15.625, 7.8125 and 3.91 ppm) of the Acacia seyal bark methanolic extract, were investigated in vitro against snail Biomphlaria Pfeiffer. A. seyal bark methanolic extract exhibited 100% mortality within 24 h, only at a concentration of 1000 ppm; and exhibited 100% mortality within 48 h, at 1000, 500, 250, 125 and 62.5 ppm. The IC50 of bark of A. seyal at 24 h and 48 h was 80.79 and 34.33 ppm, respectively. These findings prove the potent in vitro activity of A. seyal (bark) methanolic extract against Biomphlaria pfeiffer.
    Keywords: Molluscicidal activity, Acacia seyal (bark), Biomphlaria snails, in vitro, Sudan
  • Reza Tabaripoor*, Haleh Akhavan Niaki, Mohammad Reza Esmaeili Dooki, Tahereh Dadkhah, Ali Mohammad Shirafkan, Elham Ghadami Pages 80-86
    Cystic fibrosis (CF) is the most common severe autosomal recessive disorder caused by a wide spectrum of mutations in the gene encoding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The frequencies, types and distributions of mutations vary widely between different populations and ethnic groups. The aim of this study was to perform a comprehensive analysis of the CFTR gene in an Iranian heterogeneous population. 20 CF patients diagnosed according to clinical evaluation and elevated sweat chloride value and presenting no common CFTR mutation, were analyzed for mutations and polymorphisms in exons 7, 20 and 21 and parts of introns 6, 7, 19, 20 and 21 of CFTR gene using PCR-sequencing. Sequencing of exon 7 revealed the presence of two variations c.864G>A (rs766189605) and c.910C>T (rs121909011) with the frequencies of 10% and 2.5%, respectively. c.864G>A is a synonymous variant that happens in amino acid valine 318 (GTG/GTA) and c.910C>T is a pathogenic missense variant that occurs at amino acid 334 (R334W) of CFTR protein leading to the change of arginine to tryptophan. Two variations c.3780A>G (rs1800130) and c.3783흞>G (rs10155917) were also found in exon 20 and intron 20 with the frequencies of 7.5% and 5%, respectively. No mutation or polymorphism was found in exon 21. Ascertainment of CFTR mutation carrier frequencies and CF incidence among heterogeneous Iranian populations seems to be a necessity.
    Keywords: Cystic fibrosis, polymorphism, non-Caucasian, R334W
  • Jimmy Etukudo Okon* Pages 87-90
    Irish potatoes is rich in carbohydrate, with varied glucose levels. It is also a very good source of apyrase, an enzyme used for platelet storage. The aim of this study was to explore Irish potatoes as an easily available source for apyrase extraction and purification. Apyrase was extracted from 5 Kg of Irish potatoes. Platelets were derived from 5-10 ml of human whole blood and G6PD activity was assessed in platelets stored for 48 h either in presence or absence of apyrase (commercial or extracted from Irish potatoes) using fluorescent spot test and electrophoresis. Platelets stored in apyrase showed G6PD activity under both fluorescent spot test and electrophoresis method, whereas those stored without apyrase enzyme for such a time did not show G6PD activity. Therefore, potatoes could be used as a valuable source for preparing apyrase and allowing platelet storage for long duration for in vitro studies or transfusion purposes, especially in bleeding disorders which will be complicated without adequate conservative medium, due particularly to the short half life of this blood cell.
    Keywords: Potatoes, platelets, apyrase, G6PD