فصلنامه فیزیولوژی و بیوتکنولوژی آبزیان
سال دوم شماره 1 (بهار 1393)

To regulate of body fluids in the marine elasmobranches¡ blood urea level is usually high. However¡ this condensation is toxic to other vertebrates. In this investigation¡ 36 individuals of brown banded bamboo sharks (Chiloscyllium punctatum) were sampled from Darvish Creek in the north of Persian Gulf. Blood samples were taken from fish and then weighing¡ biometry and the numbering of sharks were done. The results showed that the mean of urea in blood of shark Brown banded Bamboo sharks is 196/7 ± 30/2 mM. Additionally¡ significant relationships were found between the electrolytes with urea (P

Today, the reproductive systems of fish as a valuable model to study the effects of the significant components of the vile endocrine disruption have been considered. This study was inspected the testis and ovary histopathological change in Carassius auratus (Goldfish) as a suitable alive model, exposed to two common pollutants Naphthalene, Butachlor. The fishes received Naphthalene, Butachlor (with doses of 200 μg/l, 0.28 μg/l respectively) during 15 days including control treatment (without the chemicals). At the end of experiment gonad tissue was examined after fixed by fixative, processed, and embedded in paraffin as sectioned at 6μ thickness. Further stained by Hematoxylin- Eosin general method. Histopathological lesions observed in the testes included degenerated germ cells, proliferation and hypertrophy of Sertoli cells, development of connective tissue, fibrosis and inhibited development of lobules, filled seminiferous tubules with fertile sperm (at Naphtalene treatment), seminiferous tubules with no fertile sperm in it (at Butachlor treatments). These markers weren’t observed in control group and indicate that environmental contaminants can disrupt reproductive system of the aquatic animals. Histopathological lesions observed in the ovary included degenerated ooytes, cytoplasmic retraction, adhesion of the oocytes, karyoplasmic clumping and its retraction within the oocytes and anucleated oocytes. In the ovary of control treatment, most of oocytes were at vitellogenesis stage, but in Naphthalene treatment the majority of the oocytes observed at previtellogenesis. In general it can be concluded that endocrine disruption has ability to change various stages of gonadal development and gamete development stages of males and females fish.

Light is one of the important and effective environmental factors in fish life that can affect body activity and metabolism. Present study was done to study the effects of different light colors on growth indices (Growth Rate, Specific Growth Rate and Condition Factor), Feed Conversion Ratio and Survival Rate in tiger barb (Barbus tetazone) juvenile for 6 weeks. To examine, 120 fish juvenile with average weight of 0.073±0.002 g were respectively divided in to four groups treatments including white, natural, green and blue colors (with three replicates for each treatment). Fish were fed by Biomar commercial food twice a day. Results showed that growth indices (GR, SGR) and FCR had significant difference in different light colors (P˂0.05). White and green lights, respectively, led to the highest and lowest GR and SGR in fish, however, there were similar effects of white and blue lights on growth and also green and natural lights on growth. The best feed conversion ratio was observed in natural light and there were no significant difference between other treatments. In addition, the highest condition factor was related to white and blue colors and the lowest condition factor was associated to green and natural colors. Survival rate showed no significant difference in different treatments (P>0.05). Results of present study showed environment light color can significantly affect juvenile growth of tiger barb. Therefore by using suitable light color an optimum growth could be obtained culture period.

This study was designed to investigate the effects of nano-zinc oxide on blood factors of roach. In this paper the LC50 range of concentrations of zinc oxide nanoparticles for roach was determined and mortality at 24, 48, 72 and 96 hours was computed. A separate experiment was designed based on the LC50 levels and induction of the lethal concentration for hematology parameters. Blood samples of the juveniles that exposed to sublethal concentrations (50% concentration LC50) of nano zinc oxide for 7 days and the controls (without exposure to nano-materials) were collected. The results showed that zinc nanoparticles cause changes in the blood parameters of the fish that the changes in blood indices by reducing the level of red blood cells (RBC), hemoglobin (Hemoglobin) and hematocit (Hematocrit) that of the impact the nanoparticles were located along the (P

The present study investigated the effects of short-term starvation and Re-feeding on some physiological indices of Mesopotamichthys sharpeyi (Günther, 1874) fingerlings. Three experimental groups (each in 3 replicate) including 4, 8 and 16-days of starvation were used in this study. End of starvation period and the beginning of re-feeding in all treatments were concurrently occurred. Blood samples were collected from 15 fish in each treatment (5 fish from each replicate) at the initial and the end of starvation and also end of re-feeding periods, from the caudal vasculature vein using heparinized syringes after anesthetization with an aqueous solution of 2-phenoxy-ethanol (%2). Results showed that the number of red blood cells (RBCs), Hemoglobin concentration (Hb) and Hematocrit (Ht) was significantly lower in 16-days starvation group compared to basal values (P0.05), while their number increased significantly after re-feeding period (P0.05). These findings showed that fasting up to 16 days and re-feeding could not affect physiological responses in M. sharpeyi.

Nanotechnology as a modern science is rapidly expanding which can influx many biological aspects due to large extent. For this reason, concerns about the risks and consequences of this technology are rising in environment and especially in water resources. The purpose of this study is to investigate the effect of waterborn Cuo Nanoparticles on Oncorhynchus mykiss gill tissue, to detect a suitable biomarker for noticing Copper in Water Resources. Samples weighted 18±3 gr and exposure to Cuo Nanoparticels for a week with 25, 30, 50 and 100 ml/g doses. Physicochemical properties of water were 14±2 Cº temperature, 8-8.5 pH and 270 mg/l Caco3. At the end of 7 days, 9 fish were catch randomly from each treatment and second gill from left side of fish were removed for histological review. Hyperplasia, Oedema, Epithelial lifting in secondary lamella, lamellar aneurysm and Leukocyte infiltration was observed in gill tissues. In addition, the damage was determined at various concentrations of copper nanoparticles. Result showed that Cuo Nanoparticles, as copper ions, have significant adverse effects on gill tissues of O. mykiss and gills can be a suitable biomarker for copper in water resources. However, comparison of the extent and severity of injury stated that copper nanoparticles cause much less injury than copper ions.