The effects of photoperiod on embryogenesis of microspore culture in three spring rapeseed cultivars

Application of doubled haploid method can be used for breeding objectives such ac reduction time of breeding periods. In this research، the effects of photoperiod on embryo production from microspore culture of three rapeseed cultivars; Global، Option and PF7045/91 and plantlet regeneration in embryo-derived from microspores were studied in two separate experiments. In the first experiment، embryo production were analyzed from microspores cultured in NLN-13 medium influenced by photoperiod treatments were; darkness، 16/8 (light/dark) and alternative daily darkness and 16/8 (light/dark) in a factorial experiment based on completely randomized design with two factors in 5 replications. The results showed that there were significant differences between two factors and interaction between them on embryogenesis. The numbers of embryos were increased significantly in Global and PF7045/91 cultivars under darkness condition and embryogenesis were laid in group A for this two cultivar. However، the effect of photoperiod was not significant on embryogenesis in Option cultivar. In the second experiment، the plant regeneration in embryos-derived from microspores was evaluated in a factorial experiment based on completely randomized design with two factors in 10 replications. Cultivar contain Global، Option and PF7045/91 and embryo-derived from microspores under darkness and 16/8 (light/dark) were first and second factor، respectively. The results indicated that only the cultivars had significant differences. The results also indicated PF7045/91 cultivar had the highest plantlet regeneration and was set in group A.