Genetic differentiation among three populations of Sarabi, Sangsari and Afshari guardian dogs using DNA sequencing of sexual Y chromosome

Several breeds of dogs can be found in Iran. Some of them are completely different in their phenotype while others are similar. These animals are kept in rural families as guard animals for their herds. In order to study the genetic diversity of a part of Y sex chromosome between and within the population of Sarabi, Sangsari and Afshari dogs, total number of 21 non inbred male dogs were sampled. Blood samples collected from these animals were transferred to laboratory by EDTA containers. DNA extraction was applied using extraction kits and forward and reverse primers were designed using Oligo5 software. After sequencing, results were analyzed by Finch and Mega4 softwares. Analyzing the replicated sequences showed that all of the sequences were conserved and having repeated sequences caused the existence of slip pages. In this study, both neighbor joint approach and unweighted pair-group method of the arithmetic average (UPGMA) were utilized to draw phylogenetic tree. Results showed that these three populations of dogs, based on their genetic, are completely different