Effects of iron excess on the antioxidant activity and patterns of protein electrophoresis in Oryza sativa var. Shafagh

Iron as an essential element has important roles in chloroplast development, photosynthesis, Fe-S clusters structure, electron transfer and control of redox systems. Excess iron could impair biomacromolecules due to induction of oxidative stress. Iron toxicity is not common and only possible in specific regions such as the Rice fields. The purpose of this investigations were the evaluation of the effects of iron toxicity, consequent oxidative stress and damage routes and tolerate pathways under excess iron in rice (Oryza sativa. L) Shafagh Cultivar. The experiments were carried out in completely randomized design and iron treatment was included 10 (control), 150 and 250 mg L-1 accompanied by Yoshida nutrient solution. Iron excess (250 mg L-1) caused significant reduction in plant growth specially roots. Also, antioxidant enzymes activity malondialdehyde (MDA) concentrations as a lipid peroxidation index were increased. In addition to, phenolica and hydrogen peroxide concentration increased under excess iron. In spite of decrease in total soluble protein content, some protein bands, identified in electrophoresis, enhanced under high levels of iron treatments. The electrophoresis pattern of peroxidase enzyme indicated higher activity of this enzyme under excess iron treatments. The results indicated that excess iron increased ROS production and lipid peroxidation and decreased protein content and plants growth. On the contrary, increase of peroxidase activity, phenols content reduced ROS and increased iron toxicity resistance.