Influence of Phosphorus on the Reduction of Cadmium Phytotoxicity in Spinach Grown on a Calcareous Soil

Contamination of agricultural soils by heavy metals is a serious threat from both agricultural and environmental standpoints. Among heavy metals, cadmium (Cd) toxicity for humans and plants is of great concern due to its high mobility and phytoavailability in soil even at low concentrations. Opposite to Cd, Phosphorus (P) is an essential nutrient for plant growth. A greenhouse experiment was carried out in a completely randomized design to examine the influence of Cd, P and their interaction on the growth and chemical composition of spinach grown on a calcareous soil. Treatments consisted of four Cd levels (5, 10, 20 and 40 mg/kg soil as cadmium sulfate) and four P levels (0, 20, 40 and 80 mg/kg soil as mono-calcium phosphate) in three replicates. The results indicated that 40 mg Cd significantly decreased spinach dry weight by 47% but P application decreased detrimental effect of Cd on spinach dry weight. Also, phosphorus application significantly decreased Cd concentration in spinach aerial parts by 78%. Increasing Cd levels increased concentration of this element in spinach shoots. Addition of 40 mg Cd decreased P concentration by 21.5% in aerial parts. Zinc (Zn), manganese (Mn), calcium (Ca), and nitrogen (N) concentration significantly decreased with P application but increased concentration of sodium (Na). Cadmium application decreased Zn concentration but increased Ca, Na and N concentrations in spinach. Our tentative conclusion is that P application in P-deficient soils is probably effective in decreasing Cd concentration and the detrimental effect of Cd on spinach growth, indicating a negative interaction between these elements. Prior to any fertilizer recommendation, the results of this experiment should be verified under field conditions