High frequency plant regeneration from leaf, internode and microtuber explants of potato (Solanum tuberosum L.)

Potato is one of the most important crops in Iran. Optimization of tissue culture in order to achieve high frequency regeneration is the first step for genetic transformation. Here, regeneration of two important potato cultivars that are widely cultivated in Iran (Agria and Marfona) was investigated. Internodes, leaves, and microtubers explants of potato were treated with different hormone components. It was found that in the medium A, regeneration efficiency of internodes and leaves explants at 1mg/l TDZ is higher than the other TDZ concentrations. In the medium C, regeneration percentage of internodes (95.8% in Agria, 100 % in Marfona), leaves (83.3 % in Agria, 91.6 % in Marfona) and microtubers (82.6 % in Agria, 86.3 % in Marfona) has shown more efficient than medium A. On the other hand, regeneration time in medium C was 35 days less than that in medium A. We observed different stages of somatic embryogenesis for all explants at C medium. Finally, we were recommended the medium C as an efficient medium for high regeneration of these cultivars of potato.