The effects of cold pre-treatment and induction medium on anther culture efficiency of rice

The effects of cold pre-treatment duration (8، 10، 12 & 14 days at 8oC) and different induction media (N6 and Chu) were studied on callus induction and plant regeneration of the first Iranian hybrid rice “Bahar1” anther culture. The experiment was performed in randomized complete design with three replications. Percentage of callus induction in N6 medium was higher than Chu medium، but the percentage of green plant regeneration in Chu media was significantly higher than N6 medium. In the other words embryogenic callus in chu medium was more than N6 medium. The results showed that a cold pre-treatment was essential for the induction of callus from anthers. Increasing the duration of cold pre-treatment resulted in more callus production but green plant regeneration decreased. The best duration of cold pre-treatment for percentage of green plant regeneration was 8 to 10 days at 8oC. Thus the best conditions to anther culture of hybrid rice was using of chu medium with 8 to 10 days cold pre-treatment.