Comparison of Using the two Methods Preparing of Explant in Potato by Nodal and Sprout Segments in Tissue Culture

In this research, with sterilized conditions, sprouts and plantlets producing from meristem culture were divided segments 5-7 mm in length. Segments that were acquired from sprout and plantlets were transferred to erlene dishes containing MS and Nitch & Nitch medium. Each dish was considered as plot. Treatments were sat up as a factorial experiment based on randomized complete design, cultivars in two levels (Marfona, Agria) and two levels of media culture (MS and Nitch&Nitch). Treatments were applied in three replications. After four weeks plantlets that had some leaves and reached 4 to 5 cm in length were transferred to peat pot in the greenhouse. After 70 to 80 days, minitubers were harvested from these plantlets. Data of analysis variances determined that only cultivar had significant different at 5% ά level, but effect of culture medium and interaction effect (culture medium × cultivar) was not significantly different at 5% ά level. Both Agria and Marfona in two culture media had same situation in production of plantlets.Means comparison of minituber production in greenhouse showed that Marfona produced higher minitubers than Agria.