Study of the polymorphism at the 5 flanking region of the ovine IGF-I Gene and its association with carcass traits in Zel (tailed) and Lori-Bakhtiari (fat-tailed) breed sheep using PCR-SSCP

The Insulin-Like Growth Factor I (IGF-I) Gene has a similar structure with Insulin hormone. That has an important functional in growth and development different tissue and transcribe. The IGF-I gene coded this hormone which located on Chromosome five which has six exons and five introns. The single nucleotide polymorphism was occurred in IGF-I gene and that associated with carcass traits in Zel (tailed) and Lori-Bakhtiari (fat-tailed) breed sheep with using PCR-SSCP marker. For this purpose, 4 mL of total blood was collected from the left jugular vein using vacuum tubes from Zel and Lori-Bakhtiari sheep breeds. Genomic DNA was purified from 1000-μl of blood samples that using the salting-out procedure and amplified region is located in the 5’ flanking region of the ovine IGF-I Gene. The IGF-I gene 5’flanking fragment was amplified which investigated with Single-Strand Conformational Polymorphism (SSCP) method for genotyping. Electrophoresis of PCR products were done on acrylamide gel that observed the polymorphic in this region. The frequency of patterns in Zel and Lori-Bakhtiari breeds were A (37.40%), B (41.98%), C (2.29%), D (18.32%) and A (58%), B (32%), C (4%), D (6%) respectively. Finally, for analysis of some traits was used SAS program. No significant associations of the SNPs in the 5’ flanking region of the ovine IGF-I Gene were observed for any trait.