The study of Saccharomyces boulardii effects on liver enzyme level contaminated by Salmonella typhimurium in rat

Salmonellosis is an infectious disease for all animal species which caused by different species of Salmonellae and manifested clinically by preacute septicemia or enteritis. Most recent investigation suggested that some probiotics such as Saccharomayces boulardii can inhibit multiplicity of enteropathogenic bacteria such as Salmonella in vitro and it is possible to prevent pathological changes caused by these bacteria in vivo. This prospective study was conducted to investigate the protective effects of Saccharomyces boulardii on liver pathologic lesions of Salmonella typhimurium in rat. Thirty Conventional male rats were divided in to three groups (A, B, C). ALT and AST values were determined from blood samples before administration of Saccharomyces boulardii and bacterium. Rats in group B and C received 1 ml of saline contained 107 ¬and 108 CFU/ml of Saccharomyces boulardii orally for 5 days respectively. Control group (A) received only 1 ml saline as experiment groups; On day five, all animals were exposed orally with 107 Salmonella typhimurium. On day 10, all rats inoculated with bacterium then blood sample were obtained and all animals were euthanized. In biochemical examination serum level of ALT and AST in the control group (A) significantly increased (p<0/001) but not significant changes in the experiment group (B and C) were seen. In addition ALT and AST increased level between group B and C were not significant. In other hand 108 and 107cfu/ml Saccharomyces boulardii doesn’t have any significant difrentiation for protection of Salmonellosis. This study indicates that Sacharomyces boulardii has protective effect on Salmonella typhimurium infection in rat and some serum biochemical examination confirm this result.