Effect of different levels of dietary vitamin C on the immune system and hematological parameters of Barbuas grypus

This study was conducted in order to characterize the effect of different levels of dietary vitamin C on the immune system and hematological parameters of Barbuas grypus. For this purpose 300 Barbuas grypus were divided into 15 groups (5 treatments each with 3 replicate). Five levels of vitamin C (0, 200, 400, 800, 1600 mg kg-1 diet) and their combination were used to prepare five experimental diets. The fish were fed 3% of their wet body weight /day for a 60 days period. Blood samples were obtained from six fish of each tank at the end of experiment and following parameters were measured: Red Blood Count (RBC), Packed Cell Volume (PCV), Hb, Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV). According to the results, no significant difference was observed between treatments in following parameters: Red Blood Cell Count (RBC), hemoglobin (Hb)، Mean Corpuscular Volume (MCV) and mean corpuscular hemoglobin (MCH) (P≥0. 05). Based on the results, a significant increased was observed in the Mean Corpuscular Hemoglobin Concentration (MCHC) between treatments compared to the control group (P ≤0. 05) and in the Packed Cell Volume (PCV) significant difference was observed between treatments compared to control group and the highest value was recorded in the control group (P≤0. 05)