Evaluating Helicobacter Pylori FlaA in Clinical Isolates from Alzahra Hospital, Isfahan, Iran

This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori. One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain reaction (PCR) was performed using the designed primer. PCR products showed that all extracted DNA were positive for flaA gene. We found flaA gene to be present in all clinical isolates. As it is highly similar to standard Helicobacter pylori strains, it can be used to detect the mentioned bacteria.