Comparative Studies on the Interaction of Proteinase-K with Nano-CuO and Copper Ions

The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K stability and activity. Copper oxide nanoparticles had more inhibitory and destabilization effect than copper ions on proteinase K. We used thermodynamic parameter such as Tm and ΔG0 and kinetic studies to emphases the effect of nanoparticles CuO and copper ions on stability and activity of proteinase-K.