Effect of Cultivar, Medium and Plant Growth Regulators Composition on Strawberry (Fragaria × ananassa Duch.) Anther Culture

Production of haploid plants is an efficiant method in plant breeding programs, genetics and cytogenetics studies. Effects of cultivar, medium and plant growth regulators were investigated on anther culture of strawberry. This study was carried out using two commercial cultivars of satrawberry (Selva and Paros), three medium (MS, H1 and N6) and different concentrations of IAA and BAP as factorial arrangement in completely randomized design. Androgenic anthers (%), embryogenesis (%) and callogenesis (%) were determinded and recorded after 30 days. Results showed significant differences among treatments for studied traits. In the Selva cultivar, the interaction effects of the H1 medium and hormone treatments including T3 (2 mg l-1 IAA + 1 mg l-1 BAP), T2 (1 mg l-1 IAA+ 2 mg l-1 BAP) and T1 (1 mg l-1 IAA+ 1 mg l-1 BAP) showed the highest androgenic anthers (67.8%, 59.6% and 54.3%, respectively). In the Paros cultivar, the interaction effect of H1 medium × hormone treatments including T5 (3 mg l-1 IAA+ 2 mg l-1 BAP) and T3 (2 mg l-1 IAA + 1 mg l-1 BAP) showed the highest androgenic anthers (70.5% and 67.7%, respectively). Interaction of T3 × H1 medium produced the highest embryogenesis in both cultivars (Selva: 37.8% and Paros: 42.8%). This treatment was determined as suitable treatment for anther culture in both cultivars.