Investigation of CGRP gene expression in cow liver tissue and its variety among different species of animal

Calcitonin gene-related peptide (CGRP) is a 37 amino acids neuropeptide in mammals. The pathological, CGRP is involved in regulating the release of inflammatory factors such as TNFα, IL-1β and norepinephrine - epinephrine. Since CGRP is involved in releasing of inflammatory factors and during illness, the concentration of that would change in the body, then it can be used as a diagnostic factor of disease. To investigate the expression of CGRP in the liver, at first liver sample was taken from the cows (2 cow up to 60 days and 8 slaughtered cows including bulls and cows in dry period), then the samples was added to nitrogen and immediately transferred to lab. In there RNA was extracted, and the quality of the extracted RNA was determined, then cDNA was synthesized and RT-PCR was performed. The results of this study showed that CGRP gene was expressed in the liver tissue of dairy cows up to 60 days of lactation. However, it seems this gene could not be capable to express in the liver tissues of bulls and cows in dry period. Also, in this study CGRP amino acid variety were performed by DNAMAN, MEGA and Multialine software according to its amino acids sequence in NCBI. The CGRP variety of amino acids in cattle had the most homology with sheep, mice, rats and dogs. The homology of CGRP in cattle and human is high (94 %), thus it probably can be used the human or rat kit to determine CGRP gene expression or concentration of CGRP in blood.