Determination of embryo culture supernatants biomarker level after ICSI and correlation with embryo morphology

Embryo implantation is a complex and significant problem in assisted reproductive techniques. The process of implantation depends on maternal immune system to avoid rejection of conceptus. Studies have shown that s HLA-G produced by trophoblast cells and present at the feto-maternal interface، plays an important role in the embryo implantation process and in the phenomenon of fetal allograft tolerance. The establishment of a TH-1: TH-2 cytokine balance both in the secretory endometrium and the deciduas appears to play a pivotal role in determining successful embryo implantation as well as subsequent healthy placentation. This study was carried out to compare relation between the produced biomarker of embryo culture media form ICSI method and morphological parameters in embryos. The samples were 83 media culture surrounding individual embryos 46 h and 72 h after ICSI. Soluble- HLA-G levels were evaluated by an enzyme linked immunosorbent assay [ELISA] employing monoclonal antibody MEM-G9. We correlated the presence of sHLA-G with embryo morphology. correlation was found between high quality embryos and sHLA-G levels. Our results do not show any statistical correlation between low quality embryos and sHLA-G levels detected in embryo culture supernatants. According to the results given that cmorphological parameters of embryos and sHLA-G protein in culture media، it could be used as a non-invasive marker in embryo selection