Antimicrobial activity of methyl cellulose based edible film enriched with pimpinella affinis oil on the Hypophthalmichthys molitrix fillet under refrigerator storage condition

Biodegradable polysacharyd-based film was developed by incorporating pimpinella affinis essential oil (PAO) into methyle Cellulose (MC) at level of 0.5%, 1% and 1.5% v/v. Then, its antimicrobial activity tested against 7 bacteria include Staphylococcus aureus (PTCC 1431), Pseudomonas aeruginosa (PTCC 1151), Pseudomonas putida (PTCC 1691), Esherichia coli (PTCC 3315), Listeria monocytogenes (PTCC 1163), Bacillus subtilis (PTCC 465) and Vibrio parahemoliticus Films containing PAO showed significant antibacterial activity both gram-positive and gram-negative strainsa. The Inhibitory zone in film disks were increased with concentration of pimpinella affinis oil in the polymer matrix (P<0.05). Based on results, methyl cellulose edible coating contain 1.5% pimpinella affinis essential oil was used for microbial assay hypophthalmic molitrix during cold storage (4°c) which could inhibit of total bacteria growth and pscychrophilic counts of fillet.