Effect of probiotics on metabolic profile, hs-CRP and oxidative stress in type 2 diabetic adults

No studies thus far have addressed the effects of daily consumption of multispecies probiotic supplements on the metabolic profiles، hs-CRP and oxidative stress of diabetic patients. The present study was designed to determine the effects of multispecies probiotic supplements on metabolic profiles، hs-CRP and oxidative stress in diabetic patients. Fifty-four diabetic patients 35-37 years of age participated in this randomized double-blind controlled clinical trial. The subjects were randomly assigned to consume either a multispecies probiotic supplement (n = 27) or a placebo group (n = 27) for 8 wk. The supplement contained 7 viable and freeze-dried strains of lactobacillus acidophilus (2 × 109 CFU)، lactobacillus casei (7×109 CFU)، lactobacillus rhamnosus (1. 5 × 109 CFU)، lactobacillus bulgaricus (2 × 108 CFU)، Bifidobacterium breve (2 × 1010 CFU)، Bifidobacterium longum (7 × 109 CFU)، Streptococcus thermophilus (1. 5 × 109 CFU) and 100 mg fructo-oligosaccharide in lactose as a carrier substance. Fasting blood samples were taken at baseline and after 8 wk of intervention to measure metabolic profiles، hs-CRP and biomarkers of oxidative stress. The consumption of probiotic supplements prevented a rise in the FPG (changes from baseline: +1. 54 ± 30. 73 vs. +28. 73 ± 43. 44 mg/dL، p = 0. 01) and HOMA-IR (+0. 78 ± 1. 61 vs. +2. 38 ± 3. 34، p = 0. 03) values. No significant effect was found from the probiotic supplements for serum lipid profiles and HbA1C levels. A significant decrease in serum hs-CRP levels (-777. 57 vs. 878. 72 ng/ml، p = 0. 02) was found after probiotic supplementation compared to the placebo group. Supplementation also significantly increased plasma GSH levels (240. 63 vs. -33. 46 µmol/L، p = 0. 03) compared to the placebo. No significant effect for probiotics was observed for plasma TAC and serum uric acid levels between the two groups. Multispecies probiotic supplementation for 8 wk by diabetic patients increased glycemic control، decreased serum hs-CRP and increased plasma total GSH. It did not affect serum lipid profiles or total antioxidant capacity.