Detection of Enterotoxigenic Staphylococcus aureus in Schizothorax zarudnyi Using PCR Method

Enterotoxins of Staphylococcus aureus are the main factors which cause food poisoning. Thirty five samples of Schizothorax zarudnyi were cultured by bacteriological methods and S. aureuses were identified. The isolated bacteria were evaluated by PCR for diagnosis of the gene encoding of SEA and SEB. Data were analyzed using Stata software. PCR is a rapid, sensitive, specific and inexpensive method for detecting Staphylococcal enterotoxins in food. 37.2% of fishes were contaminated by S. aureus. The PCR results showed that 14.3% of S. aureus isolates possessed the SEA gene, 8.5% had the SEB gene and 5.7% possessed both genes.