The Effects of Glucose and Xylose on Growth and Single Cell Oil Production by Selected Oleaginous Yeast Strains

Using the sugars from lignocelluloses hydrolysate is a suitable strategy for biodiesel production by yeasts. In this work، Sudan black dye and Nile red florescent dye were used for staining and selecting the oleaginous yeast strains. Growth and single cell oil production in 11 selected native yeasts and 2 yeasts from microbial collection of DSMZ were investigated by using glucose، xylose، and a mixture of both as carbon sources. The results showed that native yeasts had a good sufficient potential for lipid production in the industrial field. According to the type of strain and carbon source، lipid production، dry cell weight، lipid content، and lipid production efficiency were achieved to be 0. 21-6. 95 g/l، 0. 73-10. 9 g/l، 26. 4-81. 4%، and 6. 41-21. 95% respectively. Among the investigated yeast strains، the highest lipid content (81. 4%) and lipid production efficiency (21. 95%) were related to strain UIMC65. The highest lipid production (6. 95 g/l) was acquired in strain UIMC35 (a mixture of glucose and xylose as the carbon source) and strain UIMC74 (glucose as the carbon source). TLC and FT-IR spectroscopy techniques were used for the quality analysis of produced single cell oil by yeast strains.