Evaluation of the Effect of Enamel Surface Treatment Using Er.Cr:YSSG and Applying Two Bondings with Current Clinical Method on A Fissure Sealant Microleakage

Abstract: Introduction & The clinical success of sealant therapy depends on its ability to pro-vide a marginal integrity resulting in micro leakage absence. The aim of this study was to evaluate the effect of enamel surface treatment using Er. Cr: YSSG and applying bandings with current clinical method on a fissure sealant microleakage. In this experimental study 60 human premolar teeth were assigned to 3 groups according to the conditioning as follows: (1) 35% phosphoric acid gel etching; (2) Er،Cr: YSSG laser + applying a self-etch adhesive; and (3) Er،Cr: YSSG laser + applying margin bond. All fissures were sealed by Helioseal F according to the manufacturer''s instruc-tions. The samples were thermocycled for 500 cycles (5 degrees -55 degrees C)، and im-mersed in a 0. 5% basic fuchsin solution for 24 h. After sectioning، microleakage was evalu-ated by stereomicroscope at 6x magnification. Statistical analysis was performed with Kruskal-Wallis and Mann-Whitney U tests at a significance level of 0. 05. No dye penetration was perceived in 20%، 15%، and 0% of cases in groups 1، 2، and 3 respectively; all specimens were suffering from microleakage، in group 3. There was no statistically significant difference of microleakage scores between groups 1 and 2 (P=0. 976). in group 1 were significantly lower than those of group 3 (P=0. 016)، and those of group 2 were lower than group 3 (P=0. 009). The findings of this study showed that enamel conditioning prior to the place-ment of sealant by Er،Cr: YSSG laser combined with an self-etch adhesive prevent micro-leakage as effective as conventional acid etching.