Protective Effect of Curcumin on Hormones of Spermatogenesis and DNA Breaks in Rats Exposed to Gamma-radiation

Radiation therapy is a stage of therapeutic in the management of tumors that causes normal cells damage. So compounds with name Radio protective drugs injected before of radiotherapy that reduce cell damages and death against ionizing radiation. Curcumin is an antioxidant compound. The aim of this study is to investigate the possible protective effect of curcumin on Hormones of spermatogenesis and DNA strand break against Gamma-Radiation in rats. 70 adult male Wister rats randomly divided in to 10 groups including: control, sham, Experimental 1(treatment with maximum of cur cumin in amount of 100 mg/ml), Experimental 2 (treatment with medium of cur cumin in amount of 50 mg/ml), Experimental3(treatment with minimum of curcumin in amount of 25 mg/ml), Experimental 4 (treatment with curcumin + radiation in amount of maximum), Experimental 5 (treatment with curcumin+radiation in amount of medium), Experimental 6 (treatment with curcumin+radiation in amount of minimum), Experimental 7 (treatment with solvent+ radiation), Experimental 8 (exposure to irradiation). All of animals received for 15 days respectively as Intraperitonealinjection. Then the animals were euthanized and blood collection for FSH, LH and tesostrone hormones and extraction of DNA from bone marrow slides for molecular biology studies. ELISA hormone assay for measuring of hormones levels and agarose gel electrophesis with Ethidium Bromide-stained for separating of DNA breaks was used. Data were analyzed by SPSS 15 software and statistical tests including two-way ANOVA and Duncan test. This study showed that Gamma-radiation with dose of 2 Gy caused significantly decreased of plasma testosterone level and significantly increased of plasma LH, FSH hormones levels (p< 0.05) and many breaks in DNA molecule in irradiation and solvent + radiation groups with control. So treatment with curcumin balances these adverse effects of Gamma-radiation on testis hormones of spermatogenesis and DNA molecule and leads toward normal position.These results suggest curcumin have an ameliorative effect on concentration of plasma hormones of spermatogenesis and DNA molecule breaks against Gamma-radiation