Evaluation of colonization ability of lactobacilli isolated from traditional cheese and adherence to epithelial cells of human GI tract, Caco-2 cell line

The aim of this study was to asses’colonization ability of 7 lactobacilli strain isolated from traditional cheese in GI[1] trace. So resistance to acidic pH, bile salt and ability to adhere Caco-2 cell line was assessed in vitro. 2 hour acidic treatments lead to 0-4 log reduction of tested strains. Isolate B1D2was fully resistance and no reduction in population observed after acid treatment. Bile salt cause growth delay between 25-180 min for all strains and BH1with D= 25 ± 4.80 min was the most resistance strain. Ability to adhere Caco-2 cell line was strain depended and GI2 and SL2 were less and most adherent strain respectively. In conclusion evaluation of these 3 factor in vitro indicated that SM3, BD2, SM1, SL2 isolated from Semnan and Babol cheese have colonization ability