Characterization and Lens Fiber like Cells Differentiation of Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue and Amniotic Fluid: A Comparative Study

In this study the effect of vitreous humor on the mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and amniotic fluid to lens like cells was investigated. Inthis experimental study collected stem cells from femurs bone marrow and inguinal fat pads of mature NMRI mice and amniotic fluid of 13 days embryos of NMRI mice were cultured. The mesenchymal character of these cells was proven by flow cytometry markers like CD90, CD31. This experiment took place for 14 days with different dosages 10, 15, 20, 30, 40, 50 % of bovine vitreous humor. Express of crystalline markers were detected by immunocytochemistry in experimental and control groups. The flow cytometeric analyses of surface markers were shown expression of CD90 by bone marrow and amniotic fluid stem cells and adipose tissue stem cells (29/29%, 0/57%, 82%, respectively). Moreover, mesenchymal stem cells from these 3 sources had expression of CD31 (3/44%, 1/53%, 0/1%, respectively). Immunocytochemistry results revealed that 40 % vitreous humor in culture media fluid more inducing effect on adipose and amniotic fluid mesenchymal stem cells and 15 % vitreous humor on bone marrow stem cells in order to differentiate. According to the findings of this study, it can be concluded that MSCs derived from all sources can differentiate into lens fiber like cells by inducing effect of vitreous humor.