Isolation of infected Mycoplasma. synoviae from the ostrich's lungs in Kerman province

Mycoplasma infections are contagious infections in the ostrich. At the beginning, ostrich Mycoplasma causes disease in the respiratory which have symptoms such as inflammation of the nose, trachea; also lung damage can be related to this disease. In this research, the isolation of Mycoplasma synoviae infection of the lungs and comparison of two methods (culture and multiple chain reactions (PCR)) were examined to confirm Mycoplasma contamination of ostrich lung. A multiple random samples of different parts of lung were collected from a Kerman ostrich slaughter-house; these samples were immediately collected after the slaughter in the first six months of 1391. Samples in parallel conditions were used for two methods (culture and PCR methods). The PCR product of the 16S rRNA gene was observed by UV. The egg shape of colonies and positive culture of Mycoplasma were studied on PPLO Agar culture. In addition, the molecular results of PCR product were observed on agarose gel. Although, the past research has shown that contamination in poultry flocks in industrialized regions in the country is much more than the rate of ostrich farms in Kerman but ostrich farms could serve as a reservoir for bacterial infection to spread among industrial zones of country’s poultries, despite all preventive measures.