Isolation, identification and considering the antagonistic properties of bacteria from hot water acidic spring in Boushehr province

Aim and The aim of this study was identification and isolation of thermophilic bacteria from Dalaky hot spring located in Boushehr province by using of biochemical and molecular methods and considering of enzymatic properties, anti-bacterial and anti-fungal of isolated strains. Sampling from surface water, sludge and depth of one meter was carried out in sterile conditions.Samples were incubated for a week, then grown Bacteria were purified. Identification was done by using of biochemical and molecular methods. Antibacterial properties against Eshershia coli bacteria and Staphilococcus aureus by turbidometery method and antifungal properties against Aspergillus niger based on the power of inhibitory were studied. 12 strains (A1-A12) were isolated and purified. A1, A8, A9, A11 strains, can tolerate 20% sodium chloride salt. A1 and A11 strains are capable of growth at pH 3-12 and at temperatures of 20-56 ° C.The results of antibacterial effect against S. aureus strains showed that with increasing concentration of strain extract from % 5 to 20% in A5, A6, A7, A10, A11, A9, A12 strains, the antibacterial properties increased significantly. while just A12 strain prevented the growth of E-coli in concentration of 20%.The most effective strains against Aspergillus were A2 and A6. As A11 strains can grow in a wide range of temperature, pH, as well as the superior ability of antagonistic 16SrDNA coding region was sequenced. According to this A11 strain with 98% similarity was identified as Bacillus subtilis subsp. spizizenii strain NRRL. Because of anti-fungal and anti-bacterial effect, thermophilic bacteria is an excellent option in the treatment of bacterial diseases,in future studies.