Isolation and identification of a riboflavin producer yeast from Nectarine

Many microorganisms like fungi, bacteria and yeasts, have a natural ability to produce vitamins included vitamin B2 or riboflavin. In this regard, the present study was performed to isolation and screening for riboflavin producing yeasts from various sources of soil, leaf and fruit s. Materials and method s: samples of leaf, soil and fruits were prepared for the presence of yeasts and by its ability to produce riboflavin. After purification and enrichment of samples, in order to assay riboflavin production, spectrometry, thin layer chromatography and high performance liquid chromatography were used. Finally, the best selected isolate was identified using conventional morphological, biochemical and molecular techniques. In this study, 26 yeast strains were isolated from environmental samples, that 6 isolates showed the ability to produce riboflavin. Identification results of the best selected isolate by biochemical and phenotypic characteristics revealed that this isolate is related to Clavispora lusitaniae and considering isolation of it from nectarine, has named it Clavispora lusitaniae strain N3 (Gene accession no: JQ586258). Discussion and Although only one of the six producing strains was studied and identified, observation of ability to produce among 23% of strains showed necessity for further investigation. And according to the result of absence of viewing report about production by investigated strain, it can be said that Iran has potentiality for isolation of yeasts and is capable of producing riboflavin.