A Gene Transfer System Establishment Coexpressingof eGFP and HIF-1α in Mesenchymal Stem Cells Based on Lenti Viral Vectors

Stem cell therapy has been introduced as an innovative and promising treatment in Ischemic diseases. Mesenchymal stem cells are considered for cell therapy to some extent due to their immunemodulatory، differentiation potential، feasibility of isolation and proliferation properties. Stem cells، after transplantation، often encounter harsh and hypoxic environment in ischemic tissues، which leads to cell death and decreased therapeutic efficiency. On the other hand، the fate of stem cell viability and differentiation is still an ambiguous issue in cell therapy regenerative medicine. To overcome this problem، Hypoxic/Ischemic preconditioning has been reported as a powerful tool with beneficial effects on cell survival. The reported master regulator in this process is a transcription factor known as HIF-1α. This study aimed to over-express HIF-1α in mesenchymal stem cells along with eGFP by using lenti viral vectors. Bisistronic expression of eGFP and HIF-1α provides the possibilities of tracking the transplanted cells and mimicking the hypoxic conditions for genetically modified stem cells for future animal model studies.