The Effect of Humanin on Spatial Memory Disorder Induced by Intraventricular Injection of Streptozotocin in Rats

Backgrounds & Sporadic Alzheimer's is known as a new type of devastating disease that the impairment of the insulin signaling pathway may be one of factors causing it. The aim of study was to determine the impact of humanin on spatial memory impairment induced by intraventricular injection of streptozotocin in rats. In this experimental study 42 male rats weighing 250 to 300 g were selected and then cannule implanted bilaterally into their lateral ventricles. STZ or saline was injected in lateral ventricle every other day for the first and the third days. Humanin drug was injected at doses (0.01, 0.05, 0.1and 1 n/mol) from days four until fourteenth. From day 14th to 17th the animal spatial memory was studied using water maze method. Data were analyzed by repeated measure and one-way analysis of variance (ANOVA) followed by Tukey's test. Groups treated with humanin at doses 0.01, 0.05, 0.1 and 1 n/mol could not significant improved spatial memory deficits induced by STZ. Humanin with its known neuroprotective effects could not improve spatial memory deficits induced by intra-cerebroventricular STZ.