Investigation the effect of Lovastatin on experession of Ran protein gene in dermatophyte pathogen Trichophyton rubrum

Trichophyton rubrum is the most common cause agents of dermatophytosis in human skin and nail tissue. Some properties of T. rubrum have been investigated in molecular level; however no information is available regarding the Ran protein in this dermatophyte. The effects of lovastatin were investigated by using various concentrations of this medicine in T. rubrum cultures. Nucleic acids (RNA) were isolated from obtained mycelial mass of this fungus by standard methods. Pairs of primers were designed and synthesis from highly conserved regions of the Ran protein genes in other eukaryotic cells. Then primer and cDNA which synthesis of RNA extracted of T. rubrum utilized in PCR. The growth of fungi was decreases in this medium. Lovastatin depend on concentration reduced growth of fungi and gene expression of Ran protein gene, and with increase of drug concentration growth and gene expression were decreased.